FOXQ1 CRISPR/Cas9 KO Plasmid (h): sc-403650
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXQ1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FOXQ1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXQ1 Antibody (C-9): sc-166265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXQ1 CRISPR/Cas9 KO Plasmid (h) | sc-403650 | 20 µg | $397.00 |
Overview
FOXQ1 (forkhead box Q1) encodes a forkhead family transcription factor that regulates epithelial lineage programs, cell differentiation, and context-dependent transcriptional networks involved in motility and tissue remodeling. FOXQ1 activity has been linked to modulation of epithelial–mesenchymal transition-associated gene expression and cross-talk with Wnt/β-catenin and TGF-β signaling outputs, influencing adhesion and cytoskeletal dynamics. Dysregulated FOXQ1 expression is reported across multiple tumor types and is frequently associated with altered invasion-related transcriptional signatures and metastatic potential. As a DNA-binding transcriptional regulator, FOXQ1 is also used to interrogate promoter/enhancer logic and chromatin-associated control of epithelial gene expression.
FOXQ1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOXQ1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOXQ1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOXQ1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FOXQ1 protein expression.
This CRISPR knockout system enables efficient generation of FOXQ1-deficient cell models for investigation of FOXQ1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOXQ1 exon(s) critical for FOXQ1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOXQ1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FOXQ1 CRISPR/Cas9 KO Plasmid (h) and FOXQ1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOXQ1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FOXQ1 HDR Plasmid (h) and FOXQ1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOXQ1 homology arms to support homology-directed repair at defined FOXQ1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.