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Fluorescein di(β-D-galactopyranoside) (CAS 17817-20-8)

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Alternate Names:
FDG
Application:
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase
CAS Number:
17817-20-8
Molecular Weight:
656.59
Molecular Formula:
C32H32O15
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Fluorescein di(beta-D-galactopyranoside) is a fluorogenic substrate for beta-Galactosidase with potential use for ELISA, fluorescence microscopy or flow cytometry. It is hydrolyzed to release the fluorescent moiety, which can be used to quantify the enzymatic activity. This characteristic makes it particularly valuable in various applications within biological research and diagnostic assays. One of the primary uses of Fluorescein di(beta-D-galactopyranoside) is in the study of gene expression, where it acts as a reporter molecule. In genetic constructs where the beta-galactosidase gene is placed under the control of a specific promoter, the expression of beta-galactosidase can be induced by certain stimuli or conditions. The presence of beta-galactosidase activity can then be visualized and quantified based on the fluorescence produced from Fluorescein di(beta-D-galactopyranoside) cleavage, providing insights into the regulation and dynamics of gene expression.


Fluorescein di(β-D-galactopyranoside) (CAS 17817-20-8) References

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  2. Integration of on-column immobilized enzyme reactor in microchip electrophoresis.  |  Park, SS., et al. 2003. Electrophoresis. 24: 200-6. PMID: 12652592
  3. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells.  |  Yang, NC. and Hu, ML. 2004. Anal Biochem. 325: 337-43. PMID: 14751269
  4. Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets.  |  He, M., et al. 2005. Anal Chem. 77: 1539-44. PMID: 15762555
  5. Developing a biosensor for estrogens in water samples: study of the real-time response of live cells of the estrogen-sensitive yeast strain RMY/ER-ERE using fluorescence microscopy.  |  Wozei, E., et al. 2006. Biosens Bioelectron. 21: 1654-8. PMID: 16143503
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  7. Timing controllable electrofusion device for aqueous droplet-based microreactors.  |  Tan, WH. and Takeuchi, S. 2006. Lab Chip. 6: 757-63. PMID: 16738727
  8. Reliable transient promoter assay using fluorescein-di-beta-D-galactopyranoside substrate.  |  Ikenaka, K., et al. 1990. DNA Cell Biol. 9: 279-86. PMID: 1693513
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  13. Fluorogenic Substrates For Β-D-Galactosidases And Phosphatases Derived From Fluorescein (3, 6-Dihydroxyfluoran) And Its Monomethyl Ether  |  Boris Rotman, et al. 1963. National Academy Of Sciences. 50: 1-6.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Fluorescein di(β-D-galactopyranoside), 1 mg

sc-221617
1 mg
$89.00

Fluorescein di(β-D-galactopyranoside), 2 mg

sc-221617B
2 mg
$114.00

Fluorescein di(β-D-galactopyranoside), 5 mg

sc-221617A
5 mg
$212.00

Fluorescein di(β-D-galactopyranoside), 10 mg

sc-221617C
10 mg
$385.00

Fluorescein di(β-D-galactopyranoside), 25 mg

sc-221617D
25 mg
$877.00

Fluorescein di(β-D-galactopyranoside), 50 mg

sc-221617E
50 mg
$1574.00