fish抗体 (G-7) 推荐用于 mouse, rat 和 human 来源的fish WB, IP, IF 和 ELISA检测

fish 抗体 (G-7): sc-376211

fish抗体 (G-7) is rated 3.5 out of 5 by 6.
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说明书

    • 抗-fish 抗体 G-7 是小鼠单克隆 IgG1 κ,fish抗体, 在5篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列825-1124位于mouse物种的fish的C-terminus
    • 抗-fish 抗体 (G-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 fish
    • 抗-fish 抗体 (G-7) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取fish (G-7): sc-376211免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for fish Antibody (G-7) for WB applications. This reagent is now offered in a bundle with fish Antibody (G-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-376211: fish (G-7) mouse monoclonal antibody will work?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from Did not work for IF with MDA-MB-231 cells Tried incubating at 1:50 dilution overnight, and still no signal. Tried both in control and knock-down. Seems like it may only work for western blots. Wish I had known before I bought it!
Date published: 2019-05-28
Rated 1 out of 5 by from Did not work for WB Used a 1:300 dilution for WB with SUM159, Caco-2 and 293-T cell lysates. Did not see any band.
Date published: 2018-01-30
Rated 4 out of 5 by from Worked well for IB Picks up 2 bands approx 120-150kda in 25ug PANC-1 cell lysate after incubation 4C overnight at 1:1000.
Date published: 2017-01-25
Rated 5 out of 5 by from Sensitive and reliable Nice strong bands on Western blot using a 1:200 dilution, smart choice!
Date published: 2017-01-18
Rated 5 out of 5 by from Produced positive Western Blot data of fish Produced positive Western Blot data of fish expression in K-562 whole cell lysate. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Good Western Blot data of fish expression Good Western Blot data of fish expression in Sol8 whole cell lysate. -SCBT QC
Date published: 2013-10-08
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