Date published: 2025-9-6

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Fibronectin 抗体 (C6F10): sc-73611

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说明书
  • Fibronectin抗体(C6F10)是小鼠单克隆IgG1 κ, 在13篇文献中引用,规格为200 µg/ml
  • 针对human来源的血浆Fibronectin
  • 抗-Fibronectin 抗体 (C6F10) 推荐用于 WB, IP, IF 和 IHC(P),检测human, monkey, porcine, bovine, equine, avian 和amphibian 来源的 Fibronectin III-10; 不会与 other serum proteins产生交联反应
  • 抗Fibronectin抗体(C6F10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fibronectin (C6F10): sc-73611免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Fibronectin 抗体 (C6F10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Fibronectin 抗体 (C6F10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

纤连蛋白抗体(C6F10)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测人类纤连蛋白。抗纤连蛋白抗体(C6F10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®选项,可满足不同实验需求。纤维粘连蛋白是一种高分子量细胞外基质糖蛋白,对于细胞粘附、运动和伤口愈合至关重要,存在于大多数细胞表面、细胞外液和血浆中。作为异二聚体,纤维粘连蛋白会经历选择性剪接和糖基化,产生具有组织特异性分布和与某些恶性肿瘤相关联的各种同工型。糖基化的纤维粘连蛋白和特定的剪接变体与杜普伊特伦氏病等病症中的肌成纤维细胞表型形成有关,这凸显了纤维粘连蛋白在正常生理和病理条件下的重要性。纤维粘连蛋白(C6F10)单克隆抗体与猴子、猪、牛、马、鸟类和两栖动物组织有交叉反应,这使得该抗体在各种生物学研究应用中具有价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Fibronectin 抗体 (C6F10)

sc-73611
200 µg/ml
$316.00

Fibronectin (C6F10): m-IgG Fc BP-HRP 套装

sc-525700
200 µg Ab; 10 µg BP
$354.00

Fibronectin 抗体 (C6F10) AC

sc-73611 AC
500 µg/ml, 25% agarose
$416.00

Fibronectin 抗体 (C6F10) HRP

sc-73611 HRP
200 µg/ml
$316.00

Fibronectin 抗体 (C6F10) FITC

sc-73611 FITC
200 µg/ml
$330.00

Fibronectin 抗体 (C6F10) PE

sc-73611 PE
200 µg/ml
$343.00

Fibronectin 抗体 (C6F10) Alexa Fluor® 488

sc-73611 AF488
200 µg/ml
$357.00

Fibronectin 抗体 (C6F10) Alexa Fluor® 546

sc-73611 AF546
200 µg/ml
$357.00

Fibronectin 抗体 (C6F10) Alexa Fluor® 594

sc-73611 AF594
200 µg/ml
$357.00

Fibronectin 抗体 (C6F10) Alexa Fluor® 647

sc-73611 AF647
200 µg/ml
$357.00

Fibronectin 抗体 (C6F10) Alexa Fluor® 680

sc-73611 AF680
200 µg/ml
$357.00

Fibronectin 抗体 (C6F10) Alexa Fluor® 790

sc-73611 AF790
200 µg/ml
$357.00

Hi, I tried this antibody for porcine colon tissue lysate and it did not work. The WB results always show the roughly 50 kDa band, not 230kDa as in the datasheet. Are you sure this antibody is also reactive with porcine fibronectin?

Asked by: danK
Thank you for your question. Please contact technical service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2021-02-03

Does this antibody react with fibronectin of mouse origin, say fibronectin from 3T3? Best regards, Zheng

Asked by: Beer
Thank you for your question. This antibody is not recommended for detection of Fibronectin of mouse origin. We have many alternative Fibronectin antibodies that are recommended for mouse. Please contact our Technical Service Department for help in selecting the best antibody for your research applications.
Answered by: Technical Support
Date published: 2019-02-23

How can Fibronectin (C6F10): sc-73611 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
Thank you for your question. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Please contact our special orders department for pricing and availability of sc-73611 conjugates or consider using Fibronectin (EP5): sc-8422 , which is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please visit this link for more information: https://www.scbt.com/scbt/product/fibronectin-antibody-ep5
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 4 out of 5 by from 良好な結果ウエスタンブロッティングで使用したが、目的の分子量と概ね一致したバンドが検出された。今度は蛍光免疫染色で使用してみたい。
Date published: 2020-07-07
Rated 5 out of 5 by from GreatI used this for flow cytometry both unfixed and fixed. Worked great in both ways.
Date published: 2019-06-19
Rated 2 out of 5 by from temperamentalWorks only sometimes...we have not had much luck with this antibody. We've tried all tricks though.
Date published: 2018-12-22
Rated 4 out of 5 by from Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 4 out of 5 by from Good antibodyIt works well to detect purified fibronectin in Western blot at 1:200 dilution
Date published: 2018-03-12
Rated 5 out of 5 by from Highly recommended for IHC(P)Fantastic immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue!
Date published: 2017-01-14
Rated 5 out of 5 by from Good bands with UGood bands with U-87 MG and HT-1080 cell lysates in WB -SCBT QC
Date published: 2014-03-10
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Fibronectin 抗体 (C6F10) is rated 4.1 out of 5 by 7.
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