Date published: 2025-9-6

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FGF-1 抗体 (B-3): sc-55520

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说明书
  • FGF-1 抗体 B-3 是小鼠单克隆 IgG2b κ,FGF-1抗体, 在17篇文献中引用,规格为200 µg/ml
  • 免疫human物种的FGF-1的氨基酸16-140
  • FGF-1 抗体 (B-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 FGF-1
  • 抗FGF-1抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FGF-1 (B-3): sc-55520免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 FGF-1 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FGF-1 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

FGF-1 抗体 (B-3) 是一种小鼠单克隆 IgG2b kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和酶联免疫吸附试验 (ELISA) 检测人源 FGF-1 蛋白。FGF-1 (B-3) 抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。成纤维细胞生长因子-1(FGF-1)又称酸性 FGF,在细胞增殖、分化和存活等各种生物过程中发挥着至关重要的作用,尤其是在间质细胞、上皮细胞和神经外胚层细胞中。FGF-1 可促进血管生成和组织修复,因此是伤口愈合和发育过程中的重要因素。FGF-1 与肝素和成纤维细胞生长因子受体(FGFRs)等特定蛋白质相互作用,这些蛋白质对介导生物效应至关重要。FGFR 相互作用会触发影响细胞反应的下游信号通路,从而突显出 FGF-1 在正常生理和病理情况下的相关性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FGF-1 抗体 (B-3) 参考文献:

  1. 小鼠乳腺癌细胞依赖雄激素生长所必需的雄激素诱导生长因子的克隆和特征描述。  |  Tanaka, A., et al. 1992. Proc Natl Acad Sci U S A. 89: 8928-32. PMID: 1409588
  2. 克隆和表达与酸性和碱性成纤维细胞生长因子交叉反应的两种不同的高亲和力受体。  |  Dionne, CA., et al. 1990. EMBO J. 9: 2685-92. PMID: 1697263
  3. 一种新型成纤维细胞生长因子-1(FGF1)突变体,可作为 FGF 的颉颃物。  |  Yamaji, S., et al. 2010. PLoS One. 5: e10273. PMID: 20422052
  4. 碱性成纤维细胞生长因子细胞生物学的最新发展。  |  Rifkin, DB. and Moscatelli, D. 1989. J Cell Biol. 109: 1-6. PMID: 2545723
  5. 成纤维细胞生长因子家族新成员--与 HST 相关的 FGF.6 基因的特征。  |  Marics, I., et al. 1989. Oncogene. 4: 335-40. PMID: 2649847
  6. 通过转染卡波西肉瘤 DNA 分离出的癌基因编码一种生长因子,属于 FGF 家族。  |  Delli Bovi, P., et al. 1987. Cell. 50: 729-37. PMID: 2957062
  7. 小鼠 int-2 的序列, 拓扑结构和蛋白质编码潜力:一种由小鼠乳腺肿瘤病毒激活的假定癌基因。  |  Moore, R., et al. 1986. EMBO J. 5: 919-24. PMID: 3013624
  8. 人类 FGF-5 癌基因编码一种与成纤维细胞生长因子有关的新型蛋白质。  |  Zhan, X., et al. 1988. Mol Cell Biol. 8: 3487-95. PMID: 3211147
  9. FGF-1 调节胰腺β细胞的功能/代谢:体外研究  |  Kolodziejski, PA., et al. 2020. Gen Comp Endocrinol. 294: 113498. PMID: 32360543
  10. 通过霍诺克醇或 FGF 配体陷阱靶向 FGF1/FGFR1 轴,克服癌细胞的耐药性。  |  Szymczyk, J., et al. 2024. Front Pharmacol. 15: 1459820. PMID: 39329123
  11. 编码成纤维细胞生长因子家族第九个成员的新型细胞因子 cDNA 的分子克隆,该细胞因子具有独特的分泌特性。  |  Miyamoto, M., et al. 1993. Mol Cell Biol. 13: 4251-9. PMID: 8321227
  12. 小鼠成纤维细胞生长因子 10:cDNA 克隆, 蛋白质鉴定和 mRNA 表达调控。  |  Beer, HD., et al. 1997. Oncogene. 15: 2211-8. PMID: 9393979

订购信息

产品名称产品编号规格价格数量收藏夹

FGF-1 抗体 (B-3)

sc-55520
200 µg/ml
$316.00

FGF-1 (B-3): m-IgG Fc BP-HRP 套装

sc-525631
200 µg Ab; 10 µg BP
$354.00

FGF-1 (B-3): m-IgG2b BP-HRP 套装

sc-549598
200 µg Ab; 10 µg BP
$354.00

FGF-1 抗体 (B-3) AC

sc-55520 AC
500 µg/ml, 25% agarose
$416.00

FGF-1 抗体 (B-3) HRP

sc-55520 HRP
200 µg/ml
$316.00

FGF-1 抗体 (B-3) FITC

sc-55520 FITC
200 µg/ml
$330.00

FGF-1 抗体 (B-3) PE

sc-55520 PE
200 µg/ml
$343.00

FGF-1 抗体 (B-3) Alexa Fluor® 488

sc-55520 AF488
200 µg/ml
$357.00

FGF-1 抗体 (B-3) Alexa Fluor® 546

sc-55520 AF546
200 µg/ml
$357.00

FGF-1 抗体 (B-3) Alexa Fluor® 594

sc-55520 AF594
200 µg/ml
$357.00

FGF-1 抗体 (B-3) Alexa Fluor® 647

sc-55520 AF647
200 µg/ml
$357.00

FGF-1 抗体 (B-3) Alexa Fluor® 680

sc-55520 AF680
200 µg/ml
$357.00

FGF-1 抗体 (B-3) Alexa Fluor® 790

sc-55520 AF790
200 µg/ml
$357.00

Is the FGF-1 (B-3): sc-55520 monoclonal antibody applicable for FCM?

Asked by: jiangquan
thank you for your question. Anti-FGF-1 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2021-03-22

I am using FGF-1 (B-3): sc-55520 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Très peu sensibleAnticorps testé sur des extraits de cellules surexprimant le FGF1. La surexpression avait déjà été validée en utilisant un anticorps d'un autre fournisseur. Avec cet anticorps, une bande de très faible intensité est détectée à partir de 25 µg d'extraits après 20 minutes d'exposition.
Date published: 2017-10-11
Rated 5 out of 5 by from strong bandused for WB studies in mouse liver tissue. It displays a unique strong band when it is used
Date published: 2017-07-28
Rated 3 out of 5 by from can't get strong bands on human VSMCsI have loaded 20 ug protein, but only got faint bands.
Date published: 2017-07-19
Rated 5 out of 5 by from Great specificityBeautifully specific staining seen of cells in glomeruli when working with paraffin-embedded kidney tissue sections in IHC.
Date published: 2017-02-03
Rated 5 out of 5 by from Good staining in IHC in human endometrial samplesGood staining in IHC in human endometrial samples. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Strong band in WB in human recombinant FGFStrong band in WB in human recombinant FGF-1. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and membraneSuperb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Worked well in IHC in mouse 4T1 cellsWorked well in IHC in mouse 4T1 cells. -SCBT Publication Review
Date published: 2015-02-18
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FGF-1 抗体 (B-3) is rated 4.3 out of 5 by 9.
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