Date published: 2025-9-7
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FBXO42 Antibody (FL-6) - Western Blotting - Image 37103
FBXO42 抗体 (FL-6): sc-100737. 未转染 293T: sc-117752 (A), 转染人 FBXO42 的 293T: sc-117274 (B) 和 HeLa (C) 全细胞裂解液中 FBXO42 表达的 Western 印迹分析.
FBXO42 Antibody (FL-6) - Western Blotting - Image 37103
FBXO42 抗体 (FL-6): sc-100737. 未转染 293T: sc-117752 (A), 转染人 FBXO42 的 293T: sc-117274 (B) 和 HeLa (C) 全细胞裂解液中 FBXO42 表达的 Western 印迹分析.
FBXO42 Antibody (FL-6) - Western Blotting - Image 34111
FBXO42 抗体 (FL-6): sc-100737. 对 HeLa 全细胞裂解液中 FBXO42 的表达进行 Western 印迹分析.
FBXO42 Antibody (FL-6) - Western Blotting - Image 53344
FBXO42 抗体 (FL-6): sc-100737. 非转染: sc-117752 (A) 和人 FBXO42 转染: sc-117274 (B) 293T 全细胞裂解液中 FBXO42 表达的 Western 印迹分析.
FBXO42 抗体 (FL-6): sc-100737. 未转染 293T: sc-117752 (A), 转染人 FBXO42 的 293T: sc-117274 (B) 和 HeLa (C) 全细胞裂解液中 FBXO42 表达的 Western 印迹分析.

FBXO42 抗体 (FL-6): sc-100737

4.4(5)
寫評論提問

说明书
  • FBXO42 抗体 FL-6 是小鼠单克隆 IgG2a (kappa轻链)，FBXO42抗体，规格为100 µg/ml
  • 免疫human物种的重组FBXO42
  • 推荐用于 mouse, rat 和 human 来源的FBXO42 WB, IP 和 ELISA检测
  • 关于如何获取FBXO42 (FL-6): sc-100737的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成FBXO42 抗体 (FL-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    描述
    基因信息
    说明书与实验方案
    研究信息

    関連項目

    此外 FBXO 抗体 包括 FBXO11, FBXO18, FBXO2, FBXO22, FBXO25, FBXO28, FBXO29, FBXO3, FBXO30和FBXO4

    FBXO42抗体（FL-6）是一种IgG2a κ小鼠单克隆FBXO42抗体，可通过WB、IP和ELISA检测小鼠、大鼠和人源的FBXO42蛋白。FBXO42抗体（FL-6）可以作为非偶联的抗FBXO42抗体使用。FBXO42（仅含F-box的蛋白42），也称为FBX42，是一种含有四个Kelch重复序列和一个N端F-box结构域的717个氨基酸的蛋白，属于F-box蛋白家族。F-box蛋白是SCF（Skp1-CUL-1-F-box蛋白）型E3泛素连接酶复合物的关键组成部分，参与底物识别和泛素化的招募。它们是一个更大蛋白质家族的成员，该家族通过靶向蛋白质（如细胞周期蛋白、细胞周期蛋白依赖性激酶抑制剂、IkB-α和β-连环蛋白）参与调节多种细胞过程（包括细胞周期、免疫反应、信号级联和发育过程），以便在泛素化后被蛋白酶体降解。在肝脏、卵巢、肾脏、睾丸和成人大脑中表达水平中等，在心脏和胎儿大脑中表达水平较低，FBXO42直接与Skp1 p19和CUL-1相互作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    FBXO42 抗体 (FL-6) 参考文献:

    1. 人类 F-box 蛋白家族的鉴定。  |  Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
    2. 哺乳动物 F-box 蛋白家族。  |  Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
    3. 预测人类不明基因的编码序列。XVI.150 个新的脑部 cDNA 克隆的完整序列，它们在体外编码大型蛋白质。  |  Nagase, T., et al. 2000. DNA Res. 7: 65-73. PMID: 10718198
    4. 哺乳动物 F-box 蛋白的系统分析和命名。  |  Jin, J., et al. 2004. Genes Dev. 18: 2573-80. PMID: 15520277

    订购信息

    产品名称产品编号规格价格数量收藏夹

    FBXO42 抗体 (FL-6)

    sc-100737
    100 µg/ml
    		$333.00

    Did anybody use this antibody for immunoprecipitation? What was the concencentraion used?

    Asked by: mujib
    Thank you for your question. This mouse monoclonal antibody, sc-100737, FBXO42 (FL-6), is recommended and warrantied for IP applications. Unfortunately we do not have any citations in IP at this time. You can find the recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
    Answered by: Tech Service
    Date published: 2019-08-07

    For Western Blot, is it recommended to use denatured or non-denatured conditions with FBXO42 (FL-6): sc-100737 antibody?

    Asked by: DefinitelyNotMatt
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 5 out of 5 by from Specific for FBXO42 in Western BlotAntibody detects over-expressed human FBXO42 in transfected lysate sc-117274. -SCBT QC
    Date published: 2023-09-14
    Rated 4 out of 5 by from Works well for WB in PANC1 line.Distinct band observed, though also mixed with a ghost non-specific band at ~75 KDa. Tested FBXO42 shRNA knockdown, CRISPR-knockout (homozygous frameshift deletion), and FBXO42-overexpressed (via Lentivirus) clonal stable PANC1 lines. Used this Ab (Lot #: B1220) for WB (1:800 in 5% milk-TBST0.1%, 0.125 ug/mL final, overnight ~10 hours) to test PANC1 cell lysates. 20 ug total proteins of cell lysates were each resolved on a Tris-Glycin gel (4-12% gradient) and transferred to a PVDF membrane. Antibody was freshly diluted in milk without freeze-n-thawing.
    Date published: 2020-04-21
    Rated 5 out of 5 by from wonderful antibody1:200 diluted for wb of rat protein,the band is quite strong and special!
    Date published: 2017-12-30
    Rated 4 out of 5 by from Positive signal in WB in human transfectedPositive signal in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
    Date published: 2015-04-13
    Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
    Date published: 2013-02-05
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    FBXO42 抗体 (FL-6) is rated 4.4 out of 5 by 5.
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