Date published: 2025-9-7

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FBXO11 抗体 (E-9): sc-393229

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说明书
  • FBXO11 抗体 E-9 是小鼠单克隆 IgG2b κ,FBXO11抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的FBXO11的N-terminus附近的氨基酸90-109之间
  • FBXO11 抗体 (E-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ,检测 mouse, rat, human 和 来源的FBXO11; 也和以下物种反应,包括: 和 equine, canine, bovine, porcine and avian
  • 抗FBXO11抗体(E-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FBXO11 (E-9): sc-393229免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 FBXO11 抗体 (E-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FBXO11 抗体 (E-9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

FBXO11抗体(E-9)是一种IgG2b κ小鼠单克隆FBXO11抗体(也称为FBX11抗体或VIT-1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的FBXO11蛋白。FBXO11抗体(E-9)既可以非偶联的抗FBXO11抗体形式提供,也可以多种偶联形式的抗FBXO11抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。F-box蛋白是SCF(Skp1-CUL-1-F-box蛋白)型E3泛素连接酶复合物的关键组分,参与底物识别和泛素化的招募。它们是更大蛋白质家族的成员,通过靶向蛋白质(如细胞周期蛋白、细胞周期蛋白依赖性激酶抑制剂、IκB-α和β-连环蛋白)进行蛋白酶体降解,从而参与调控多种细胞过程(包括细胞周期、免疫反应、信号级联和发育事件)。FBXO11(仅含F-box的蛋白11),也称为VIT1(白癜风相关蛋白1),是一种由927个氨基酸组成的核蛋白,含有一个UBR型锌指、一个F-box结构域和19个PbH1重复序列。FBXO11参与蛋白质泛素化,作为SCF复合物的底物识别组分,被认为可以与p53结合并抑制其转录活性。FBXO11的表达减少与白癜风有关,白癜风是一种以皮肤进行性脱色为特征的疾病。由于可选择性剪接事件,FBXO11存在多种亚型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FBXO11 抗体 (E-9) 参考文献:

  1. 人类 F-box 蛋白家族的鉴定。  |  Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
  2. 哺乳动物 F-box 蛋白家族。  |  Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
  3. VIT1',一个与白癜风相关的新基因。  |  Le Poole, IC., et al. 2001. Pigment Cell Res. 14: 475-84. PMID: 11775060
  4. 哺乳动物 F-box 蛋白的系统分析和命名。  |  Jin, J., et al. 2004. Genes Dev. 18: 2573-80. PMID: 15520277
  5. FBXO11/PRMT9是一种新的蛋白质精氨酸甲基转移酶,可均二甲基化精氨酸残基。  |  Cook, JR., et al. 2006. Biochem Biophys Res Commun. 342: 472-81. PMID: 16487488
  6. FBXO11 基因与慢性中耳炎伴渗出和复发性中耳炎的缔合作用:明尼苏达 COME/ROM 家族研究。  |  Segade, F., et al. 2006. Arch Otolaryngol Head Neck Surg. 132: 729-33. PMID: 16847180
  7. FBXO11 可促进 p53 的 Neddylation 并抑制其转录活性。  |  Abida, WM., et al. 2007. J Biol Chem. 282: 1797-804. PMID: 17098746
  8. 针对识别 N 端规则途径 E3 成分的合成异价抑制剂。  |  Lee, MJ., et al. 2008. Proc Natl Acad Sci U S A. 105: 100-5. PMID: 18162545

订购信息

产品名称产品编号规格价格数量收藏夹

FBXO11 抗体 (E-9)

sc-393229
200 µg/ml
$316.00

FBXO11 (E-9): m-IgG Fc BP-HRP 套装

sc-526229
200 µg Ab; 10 µg BP
$354.00

FBXO11 (E-9): m-IgG2b BP-HRP 套装

sc-549697
200 µg Ab; 10 µg BP
$354.00

FBXO11 抗体 (E-9) AC

sc-393229 AC
500 µg/ml, 25% agarose
$416.00

FBXO11 抗体 (E-9) HRP

sc-393229 HRP
200 µg/ml
$316.00

FBXO11 抗体 (E-9) FITC

sc-393229 FITC
200 µg/ml
$330.00

FBXO11 抗体 (E-9) PE

sc-393229 PE
200 µg/ml
$343.00

FBXO11 抗体 (E-9) Alexa Fluor® 488

sc-393229 AF488
200 µg/ml
$357.00

FBXO11 抗体 (E-9) Alexa Fluor® 546

sc-393229 AF546
200 µg/ml
$357.00

FBXO11 抗体 (E-9) Alexa Fluor® 594

sc-393229 AF594
200 µg/ml
$357.00

FBXO11 抗体 (E-9) Alexa Fluor® 647

sc-393229 AF647
200 µg/ml
$357.00

FBXO11 抗体 (E-9) Alexa Fluor® 680

sc-393229 AF680
200 µg/ml
$357.00

FBXO11 抗体 (E-9) Alexa Fluor® 790

sc-393229 AF790
200 µg/ml
$357.00

FBXO11 (E-9) 中和勝肽

sc-393229 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-393229 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Features of FBXO11(sc-393229)FBXO11 I bought the antibody two months ago, and the results of my IHC experiment using FBXO11 (sc-393229) was relatively better. Here is the detail. 1.Antibody concentrations : 1/250 2.tissue : invasive breast cancer;normal breast tissue 3.Results: 1)high expression of FBXO11 in breast tumor tissue, not expressed in normal tissues ; 2)reduced expression of FBXO11 in carcinoma after Neoadjuvant Chemotherapy. Experimental Procedure 1. Dry frozen tissue sections of 8 µm thickness at room temperature for 2 hours. 2. Fix sections with 4% paraformaldehyde for 15 minutes at room temperature. 3. Wash slides 2X, 4 minutes each, with TBS. 4. Counterstain for 1 minute with hematoxylin. 5. Dehydrate slides with sequential ethanol washes of 1 minute each starting with 75%, followed by 80% 、85%、90%、95%finishing with a 100% ethanol wash. 6. Block endogenous peroxidase by incubating 30 minutes in TBS. 7. Wash slides 3X, 3 minutes each, with TBS. 8. Block non-specific binding sites with 1/250diluted Human anti-mouse antibody for 20 minutes. 9. 1 hour at room temperature, with the appropriate antibody. 10. Wash slides 4 times in TBS, incubate the slides with biotinylated secondary antibody for 30 minutes. 11. Add avidin-biotin-peroxidase reagents, and reveal the resulting peroxidase activity by incubating the slides with a 0.5 mg HRP substrate solution . 12. Wash slides 4X in TBS. 13. Counterstain for 1 minute with hematoxylin. 14. Dehydrate slides with sequential ethanol washes of 1 minute each starting with 75%, followed by 80% 、85%、90%、95%finishing with a 100% ethanol wash. 15. Seal slides. 16. Analyze by optical microscopy.
Date published: 2018-05-02
Rated 5 out of 5 by from Produced positive Western blot data of FBXO11Produced positive Western blot data of FBXO11 expression in NIH/3T3, C32 and SK-MEL-28 nuclear extracts and HeLa whole cell lysate . -SCBT QC
Date published: 2014-02-07
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FBXO11 抗体 (E-9) is rated 4.5 out of 5 by 2.
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