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EpoR Antibody (D-5) - Western Blotting - Image 389345 — EpoR 抗体 (D-5) HRP: sc-365662 HRP. K-562 + GMCSF (A), HeLa (B), A-431 (C) 和 HL-60 (D) 全细胞裂解液中 EpoR 表达的直接 Western 印迹分析.

EpoR 抗体 (D-5) HRP: sc-365662 HRP. K-562 + GMCSF (A), HeLa (B), A-431 (C) 和 HL-60 (D) 全细胞裂解液中 EpoR 表达的直接 Western 印迹分析.

EpoR 抗体 (D-5): sc-365662

Name: EpoR抗体 (D-5)
Brand: SCBT
SKU: sc-365662
Price: 316.0 USD
Availability: InStock

4.1(8)

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说明书

EpoR 抗体 D-5 是小鼠单克隆 IgG₃ κ，EpoR抗体, 在8篇文献中引用，规格为200 µg/ml
特定于human起源的EpoR的C-terminal细胞质域内的491-513之间的表位图谱
推荐用于 human 来源的EpoR WB, IP, IF 和 ELISA检测
另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor^® 488 或Alexa Fluor^® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
关于如何获取EpoR (D-5): sc-365662的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG₃ BP-HRP是EpoR Antibody (D-5) 适用于 WB 应用。的首选辅助检测试剂，该试剂现与EpoR Antibody (D-5) 搭配使用（请参阅下面的订购信息）。

查看产品引用文献(7)

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EpoR 抗体 (D-5) 参考文献:

抗 Epo 受体抗体不能预测 Epo 受体的表达。 | Elliott, S., et al. 2006. Blood. 107: 1892-5. PMID: 16249375
白细胞介素 6 信号转导子 gp130 的关键细胞质区域在细胞因子受体家族中是保守的。 | Murakami, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 11349-53. PMID: 1662392
促红细胞生成素受体的细胞质区域包含非重叠的正性和负性生长调节结构域。 | D'Andrea, AD., et al. 1991. Mol Cell Biol. 11: 1980-7. PMID: 1848667
细胞因子受体超家族的结构设计和分子进化。 | Bazan, JF. 1990. Proc Natl Acad Sci U S A. 87: 6934-8. PMID: 2169613
EpoR-tdTomato-Cre小鼠有助于识别组织巨噬细胞和造血细胞亚群中的EpoR表达。 | Zhang, H., et al. 2021. Blood. 138: 1986-1997. PMID: 34098576
EpoR刺激小鼠和人类红细胞生成过程中的快速循环和红细胞增大 | Hidalgo, D., et al. 2021. Nat Commun. 12: 7334. PMID: 34921133
原发性家族性红细胞增多症中两个新型EPOR基因变异的鉴定：病例报告和文献综述。 | Lo Riso, L., et al. 2022. Genes (Basel). 13: PMID: 36292571
促红细胞生成素通过EPO-EpoR-RUNX1轴调节能量代谢。 | Yin, W., et al. 2024. Nat Commun. 15: 8114. PMID: 39284834
各种人类 Epo 反应细胞系和转染人类 Epo 受体 cDNA 的细胞中三种促红细胞生成素（Epo）结合蛋白的特征。 | Takahashi, T., et al. 1995. Blood. 85: 106-14. PMID: 7803787
红细胞生成素受体功能的激活和抑制：受体二聚化的作用。 | Watowich, SS., et al. 1994. Mol Cell Biol. 14: 3535-49. PMID: 8196600
在与其他细胞因子受体具有同源性的区域发生点突变，使促红细胞生成素受体功能失活。 | Miura, O., et al. 1993. Mol Cell Biol. 13: 1788-95. PMID: 8382775
红细胞生成素受体的结构, 功能和激活。 | Youssoufian, H., et al. 1993. Blood. 81: 2223-36. PMID: 8481505

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
EpoR 抗体 (D-5)	sc-365662	200 µg/ml	$316.00
EpoR (D-5): m-IgG₃ BP-HRP 套装	sc-550571	200 µg Ab; 40 µg BP	$354.00
EpoR 抗体 (D-5) AC	sc-365662 AC	500 µg/ml, 25% agarose	$416.00
EpoR 抗体 (D-5) Alexa Fluor^® 488	sc-365662 AF488	200 µg/ml	$357.00
EpoR 抗体 (D-5) Alexa Fluor^® 647	sc-365662 AF647	200 µg/ml	$357.00
EpoR 抗体 (D-5) FITC	sc-365662 FITC	200 µg/ml	$330.00
EpoR 抗体 (D-5) HRP	sc-365662 HRP	200 µg/ml	$316.00
EpoR (D-5) 中和勝肽	sc-365662 P	100 µg/0.5 ml	$68.00
EpoR 抗体 (D-5) PE	sc-365662 PE	200 µg/ml	$343.00

I used to use M-20 and C-20 to detect total EPOR in my IP samples, however, they are all discontinued, i tried this D-5 many times, but it doesn´t work at all. Do you have other recommendations? I use human cell line ASE2.

Asked by: ck5458

Thank you for your question. We are sorry to hear that this antibody is not performing well in your experiments. We currently do not offer any other antibodies specific against this epitope. If you contact our technical service, we may be able to help you optimize your protocol and make the antibody work. You can also find our recommended protocol for IP by following this link: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots

Answered by: Technical Support Europe

Date published: 2019-02-19

All of the other antibodies for rat EPO-R of your company are now discontinued. Does this antibody sc-365662 cross-react with rat EPO-R in western blot ?

Asked by: Asaine

Thank you for your question. We have not yet tested this antibody, EpoR (D-5): sc-365662, for reactivity with EpoR of mouse or rat origin. Therefore, it is not a recommended species at this time. Unfortunately, we do not offer any alternative antibodies for rat EpoR at this time.

Answered by: Technical Support

Date published: 2017-10-04

Is this antibody good for flow cytometry?

Asked by: Yenling

Thank you for your question. Unfortunately this antibody has not been tested for Flow Cytometry. However, we would be glad to send you a sample of it to try, because it is definitely possible that it works great for Flow cytometry, it just has not been tested. If you would like to try a sample, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com

Answered by: Tech Service

Date published: 2017-06-20

What application is the blocking peptide sc-365662 P appropriate for?

Asked by: jerojero

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-24

Rated 3 out of 5 by Abpc from Free sample worked okay!Thanks Santacruz! The free sample of your antibody worked okay for IHC (1:100)

Date published: 2018-02-22

Rated 4 out of 5 by Vickie from Nice worked on tissue homogenateI used it on WB of kidney tissue homogenate. It worked well with few unspecific binding. However, when I apply it to tubular cell line lysis in WB, I did not see specific band.

Date published: 2017-07-14

Rated 5 out of 5 by Wentian from play good WBWe used this antibody 1:1000 for WB and got a specific band between 55kd and 70kd.

Date published: 2017-04-29

Rated 5 out of 5 by Qingwen Meng from IHCI used this antibody in IHC (dilution 1:100) and got good staining in human stomach tissue.

Date published: 2017-02-25

Rated 5 out of 5 by zm65711 from A clean band can be seen.Antibody use many times, bought two antibodies but different batches this time, the quality is stable.

Date published: 2017-02-13

Rated 5 out of 5 by Trav11 from Good for WBThe EpoR Antibody (D-5): sc-365662 provides good data for Western blotting applications, showing strong band with minimal background.

Date published: 2017-01-27

Rated 1 out of 5 by elliott10 from Misleading informationThe positive control cell types on the gel express little EpoR and the band is the wrong size indicating the antibody is cross-reacting with a non-EpoR protein. This indicates there will be problems with false-positive results. SCBT should add a real positive control on the website to justify the conclusions.

Date published: 2016-09-10

Rated 5 out of 5 by JM from Produced nice Western blot data of EpoR expressionProduced nice Western blot data of EpoR expression in GM-CSF treated K-562 and Jurkat whole cell lysates. -SCBT QC

Date published: 2013-05-21

EpoR 抗体 (D-5) is rated 4.1 out of 5 by 8.

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EpoR 抗体 (D-5): sc-365662

EpoR 抗体 (D-5) 参考文献:

EpoR 抗体 (D-5) 规格

替代产品名称: EpoR

产品克隆名称: D-5

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₃ kappa light chain

氨基酸: 491-513

抗原表位靶标: EpoR

抗原表位种属: human

产品反应性/检测: EpoR

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 8篇各种科学出版物的引文

EpoR 抗体 (D-5)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

EpoR 抗体 (D-5)

EpoR (D-5): m-IgG₃ BP-HRP 套装

EpoR 抗体 (D-5) AC

EpoR 抗体 (D-5) Alexa Fluor^® 488

EpoR 抗体 (D-5) Alexa Fluor^® 647

EpoR 抗体 (D-5) FITC

EpoR 抗体 (D-5) HRP

EpoR (D-5) 中和勝肽

EpoR 抗体 (D-5) PE

I used to use M-20 and C-20 to detect total EPOR in my IP samples, however, they are all discontinued, i tried this D-5 many times, but it doesn´t work at all. Do you have other recommendations? I use human cell line ASE2.

All of the other antibodies for rat EPO-R of your company are now discontinued. Does this antibody sc-365662 cross-react with rat EPO-R in western blot ?

Is this antibody good for flow cytometry?

What application is the blocking peptide sc-365662 P appropriate for?

EpoR 抗体 (D-5): sc-365662

EpoR 抗体 (D-5) 参考文献:

EpoR 抗体 (D-5) 规格

替代产品名称: EpoR

产品克隆名称: D-5

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG3 kappa light chain

氨基酸: 491-513

抗原表位靶标: EpoR

抗原表位种属: human

产品反应性/检测: EpoR

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG3 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 8篇各种科学出版物的引文

EpoR 抗体 (D-5)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF647: Alexa Fluor® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

EpoR 抗体 (D-5)

EpoR (D-5): m-IgG3 BP-HRP 套装

EpoR 抗体 (D-5) AC

EpoR 抗体 (D-5) Alexa Fluor® 488

EpoR 抗体 (D-5) Alexa Fluor® 647

EpoR 抗体 (D-5) FITC

EpoR 抗体 (D-5) HRP

EpoR (D-5) 中和勝肽

EpoR 抗体 (D-5) PE

I used to use M-20 and C-20 to detect total EPOR in my IP samples, however, they are all discontinued, i tried this D-5 many times, but it doesn&acute;t work at all. Do you have other recommendations? I use human cell line ASE2.

All of the other antibodies for rat EPO-R of your company are now discontinued. Does this antibody sc-365662 cross-react with rat EPO-R in western blot ?

Is this antibody good for flow cytometry?

What application is the blocking peptide sc-365662 P appropriate for?

抗体同型: IgG₃ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

EpoR 抗体 (D-5)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

EpoR (D-5): m-IgG₃ BP-HRP 套装

EpoR 抗体 (D-5) Alexa Fluor^® 488

EpoR 抗体 (D-5) Alexa Fluor^® 647

I used to use M-20 and C-20 to detect total EPOR in my IP samples, however, they are all discontinued, i tried this D-5 many times, but it doesn´t work at all. Do you have other recommendations? I use human cell line ASE2.