Date published: 2025-9-6

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ephrin-B1 抗体 (C-6): sc-515264

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说明书
  • ephrin-B1 抗体 C-6 是小鼠单克隆 IgG1 κ,ephrin-B1抗体, 在4篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列171-240位于human物种的ephrin-B1的C-terminus附近
  • ephrin-B1 抗体 (C-6) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 ephrin-B1
  • 抗ephrin-B1抗体(C-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ephrin-B1 (C-6): sc-515264免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是ephrin-B1 Antibody (C-6) for WB applications. 的首选辅助检测试剂,这些试剂现与ephrin-B1 Antibody (C-6) 打包提供(请参阅下面的订购信息)。

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関連項目

ephrin-B1抗体(C-6)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人类来源的ephrin-B1。ephrin- B1 单克隆抗体(C-6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。作为 Eph 受体配体的表皮生长因子(ephrin)是重要的细胞表面蛋白,根据其结构和功能特征分为 ephrin-A 和 ephrin-B。ephrin-B1 是一种跨膜蛋白,具有保守的细胞质酪氨酸残基,在与 EphB 受体结合时发生磷酸化,从而引发一系列细胞内信号传导事件。这种磷酸化作用促进了ephrin-B1和EphB受体之间的双向信号传导,从而影响细胞附着和迁移等多种细胞过程。ephrin-B1在发育中的视网膜中尤为重要,因为ephrin-B1在沿背侧-腹侧轴的视网膜轴突映射中起着至关重要的作用,而轴突映射对于视觉系统的正常发育至关重要。了解ephrin-B1的相互作用和信号传导通路有助于深入了解发育生物学,以及这些通路在疾病中断裂时的潜在作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ephrin-B1 抗体 (C-6) 参考文献:

  1. Eph 受体和ephrins 限制了细胞间的交融和交流。  |  Mellitzer, G., et al. 1999. Nature. 400: 77-81. PMID: 10403252
  2. Eph 受体和ephrins  |  Jensen, PL. 2000. Stem Cells. 18: 63-4. PMID: 10661574
  3. 神经组织中活化的 ephrin-B1 和 ephB2 的体内酪氨酸磷酸化位点。  |  Kalo, MS., et al. 2001. J Biol Chem. 276: 38940-8. PMID: 11466320
  4. Ephrin-B1 可传递信号,激活整合素介导的迁移, 附着和血管生成。  |  Huynh-Do, U., et al. 2002. J Cell Sci. 115: 3073-81. PMID: 12118063
  5. 人主动脉内皮细胞由ephrin-B1诱导的膜皱褶和病灶复合体组装需要适配蛋白Crk。  |  Nagashima, K., et al. 2002. Mol Biol Cell. 13: 4231-42. PMID: 12475948
  6. Ephrin-B1反向信号通过一种独立于酪氨酸磷酸化的新机制激活JNK。  |  Xu, Z., et al. 2003. J Biol Chem. 278: 24767-75. PMID: 12709432
  7. Tiam1介导ephrin-B1和EphA2诱导的神经元外生。  |  Tanaka, M., et al. 2004. EMBO J. 23: 1075-88. PMID: 14988728
  8. ephrin-B1(EFNB1)是组织边界形成的标志物,它的突变会导致颅前鼻综合征。  |  Twigg, SR., et al. 2004. Proc Natl Acad Sci U S A. 101: 8652-7. PMID: 15166289
  9. RNF186调节结肠上皮细胞中EFNB1(表皮生长因子B1)-EPHB2诱导的自噬,以维持肠道稳态。  |  Zhang, H., et al. 2021. Autophagy. 17: 3030-3047. PMID: 33280498
  10. Ephrin B1 调节视网膜 Müller 细胞的炎症通路  |  Liu, L., et al. 2024. J Diabetes Clin Res. 6: 1-7. PMID: 38501146
  11. ZIP4抑制Ephrin-B1泛素化,激活Wnt5A/JNK/ZEB1,促进肝癌转移。  |  Guo, H., et al. 2024. Genes Dis. 11: 101312. PMID: 39040931
  12. EphB受体酪氨酸激酶配体在发育中视网膜构造系统中的分级和层状特异性分布。  |  Braisted, JE., et al. 1997. Dev Biol. 191: 14-28. PMID: 9356168

订购信息

产品名称产品编号规格价格数量收藏夹

ephrin-B1 抗体 (C-6)

sc-515264
200 µg/ml
$316.00

ephrin-B1 (C-6): m-IgG Fc BP-HRP 套装

sc-528011
200 µg Ab; 10 µg BP
$354.00

ephrin-B1 (C-6): m-IgG1 BP-HRP 套装

sc-533384
200 µg Ab; 20 µg BP
$354.00

ephrin-B1 抗体 (C-6) AC

sc-515264 AC
500 µg/ml, 25% agarose
$416.00

ephrin-B1 抗体 (C-6) HRP

sc-515264 HRP
200 µg/ml
$316.00

ephrin-B1 抗体 (C-6) FITC

sc-515264 FITC
200 µg/ml
$330.00

ephrin-B1 抗体 (C-6) PE

sc-515264 PE
200 µg/ml
$343.00

ephrin-B1 抗体 (C-6) Alexa Fluor® 488

sc-515264 AF488
200 µg/ml
$357.00

ephrin-B1 抗体 (C-6) Alexa Fluor® 546

sc-515264 AF546
200 µg/ml
$357.00

ephrin-B1 抗体 (C-6) Alexa Fluor® 594

sc-515264 AF594
200 µg/ml
$357.00

ephrin-B1 抗体 (C-6) Alexa Fluor® 647

sc-515264 AF647
200 µg/ml
$357.00

ephrin-B1 抗体 (C-6) Alexa Fluor® 680

sc-515264 AF680
200 µg/ml
$357.00

ephrin-B1 抗体 (C-6) Alexa Fluor® 790

sc-515264 AF790
200 µg/ml
$357.00

Has ephrin-B1 Antibody (C-6): sc-515264 , been tested flowcytometry applications?

Asked by: hansas
Thank you for your question. We have not yet tested this mouse monoclonal antibody, ephrin-B1 (C-6): sc-515264, for flow cytometry, so this is not a recommended application at this time. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to receive a free sample to test in this application. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-07-28

What is the recommended starting dilution for Western blot for sc-515264: ephrin-B1 (C-6) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with HCT116 cell lysate in published research. -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from Does not work in FCM on HFFFsThis antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:10
Date published: 2019-03-14
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ephrin-B1 抗体 (C-6) is rated 3.0 out of 5 by 2.
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