Date published: 2025-9-6
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ephrin-A5 Antibody (RR-7) - Western Blotting - Image 368201
ephrin-A5 抗体 (RR-7): sc-81945. 小鼠眼球 (A) 和大鼠眼球 (B) 组织提取物中 ephrin-A5 表达的 Western 印迹分析.
ephrin-A5 Antibody (RR-7) - Western Blotting - Image 368201
ephrin-A5 抗体 (RR-7): sc-81945. 小鼠眼球 (A) 和大鼠眼球 (B) 组织提取物中 ephrin-A5 表达的 Western 印迹分析.
ephrin-A5 Antibody (RR-7) - Western Blotting - Image 33596
ephrin-A5 抗体 (RR-7): sc-81945. Western 印迹分析 NIH/3T3 全细胞裂解液中 ephrin-A5 的表达.
ephrin-A5 Antibody (RR-7) - Western Blotting - Image 396363
ephrin-A5 抗体 (RR-7): sc-81945. Western 印迹分析 NIH/3T3 全细胞裂解液中 ephrin-A5 的表达.
ephrin-A5 抗体 (RR-7): sc-81945. 小鼠眼球 (A) 和大鼠眼球 (B) 组织提取物中 ephrin-A5 表达的 Western 印迹分析.

ephrin-A5 抗体 (RR-7): sc-81945

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寫評論提問

说明书
  • ephrin-A5 抗体 RR-7 是小鼠单克隆 IgG2a κ，ephrin-A5抗体, 在3篇文献中引用，规格为100 µg/ml
  • 针对部分重组蛋白，其氨基酸序列在ephrin-A5的human起源的114-203范围内
  • 推荐用于 mouse, rat 和 human 来源的ephrin-A5 WB, IP 和 ELISA检测
  • 关于如何获取ephrin-A5 (RR-7): sc-81945的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成ephrin-A5 抗体 (RR-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
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研究信息

関連項目

此外 Ephrin 抗体包括 ephrin-A1, ephrin-A3, ephrin-A5, ephrin-B1, ephrin-B2 和 ephrin-B3

ephrin-A5抗体（RR-7）是一种IgG2a κ小鼠单克隆ephrin-A5抗体（也称为Ephrin A5抗体或EFNA5抗体），可通过WB、IP和ELISA检测小鼠、大鼠和人源的ephrin-A5蛋白。ephrin-A5抗体（RR-7）可作为非偶联抗ephrin-A5抗体提供。Eph亚家族代表了迄今为止发现的最大的受体蛋白激酶组。越来越多的证据表明，它们参与中枢神经系统功能和发育。Eph受体的配体包括ephrin-A1（LERK-1/B61），被鉴定为EphA2（Eck）受体的配体；ephrin-A2（ELF-1），被鉴定为EphA3和EphA4（Sek）受体的配体；ephrin-A3（LERK-3），被鉴定为EphA5（Ehk1）和EphA3（Hek）受体的配体；ephrin-A4（LERK-4），被鉴定为EphA3受体的配体；ephrin-A5（AL-1），被鉴定为EphA5（REK7）的配体；ephrin-B1（LERK-2），被鉴定为EphB1（Elk）和EphB2（Cek5）受体的配体；ephrin-B2（LERK-5），被鉴定为EphB1、EphB3（Cek10）和EphB2受体的配体；以及最近鉴定的配体ephrin-B3（LERK-8），被鉴定为EphB1的配体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ephrin-A5 抗体 (RR-7) 参考文献:

  1. ephrin-A2和ephrin-A5引导对侧靶向，但不引导腹侧耳蜗核轴突的地形图绘制。  |  Abdul-Latif, ML., et al. 2015. Neural Dev. 10: 27. PMID: 26666565
  2. ephrin-A5的异常表达影响先天性甲状腺功能减退症大鼠的大脑发育  |  Suo, G., et al. 2018. Neuroreport. 29: 877-882. PMID: 29762250
  3. 减少 ephrin-A5 会加剧肌萎缩性脊髓侧索硬化症的病情发展。  |  Rué, L., et al. 2019. Acta Neuropathol Commun. 7: 114. PMID: 31300041
  4. Ephrin-A5通过提高Neogenin的可用性来加强netrin-1轴突导向。  |  Croteau, LP., et al. 2019. Sci Rep. 9: 12009. PMID: 31427645
  5. ADAM10裂解的ephrin-A5有助于前列腺癌转移。  |  Cai, C., et al. 2022. Cell Death Dis. 13: 453. PMID: 35551177
  6. ELF-1的鉴定和克隆，ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
  7. ELF-2是Eph配体家族的一个新成员，在小鼠胚胎后脑和新形成的体节区域节段性表达。  |  Bergemann, AD., et al. 1995. Mol Cell Biol. 15: 4921-9. PMID: 7651410
  8. 克隆参与轴突束形成的 Eph 相关酪氨酸激酶受体配体 AL-1。  |  Winslow, JW., et al. 1995. Neuron. 14: 973-81. PMID: 7748564
  9. 受体酪氨酸激酶 hek 和 elk 的配体：分离编码蛋白质家族的 cDNA。  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
  10. eph相关酪氨酸激酶受体配体家族的分子特征。  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404
  11. B61 是 ECK 受体蛋白酪氨酸激酶的配体。  |  Bartley, TD., et al. 1994. Nature. 368: 558-60. PMID: 8139691
  12. Elk-L3是Eph家族受体酪氨酸激酶的一种新型跨膜配体，在胚胎底板, 顶板和后脑节段中表达。  |  Gale, NW., et al. 1996. Oncogene. 13: 1343-52. PMID: 8808709

订购信息

产品名称产品编号规格价格数量收藏夹

ephrin-A5 抗体 (RR-7)

sc-81945
100 µg/ml
$333.00

Do you the epitope against which this Mab is directed?

Asked by: Patricia Maness
Thank you for your question. This antibody was made against a recombinant protein mapping within amino acids 114-203 of human ephrin-A5. The exact epitope has not been mapped.
Answered by: Technical Support
Date published: 2019-01-26

For Western Blot, is it recommended to use denatured or non-denatured conditions with ephrin-A5 (RR-7): sc-81945 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotThis antibody works well in mouse and rat eye tissue extracts. -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from not specific for Ephrin-A5This antibody gave a strong band around 30 Kd. However, it is not specific for Ephrin-A5 as EphrinA5 null mouse tissue (in which a big portion of Ephrin-A5 protein has bee deleted) showed the same band.
Date published: 2018-07-24
Rated 5 out of 5 by from Good antibodyIF gives specific staining. -- tested on cryosections from 4% PFA fixed mouse embryonic brain. Use 1:200 with 5 min antigen retrieval (citric buffer, PH 6.0).
Date published: 2017-10-11
Rated 4 out of 5 by from Band at expected molecular weight in westernBand at expected molecular weight in western blot analysis of ephrin-A5 expression in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2013-10-09
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ephrin-A5 抗体 (RR-7) is rated 3.8 out of 5 by 4.
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