Date published: 2025-9-7
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EphA4 Antibody (D-4) - Western Blotting - Image 378908
EphA4 抗体 (D-4): sc-365503. MOLT-4 (A), Ramos (B), Hep G2 (C), SK-BR-3 (D), HL-60 (E) 和 K-562 (F) 全细胞裂解液中 EphA4 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
EphA4 抗体 (D-4): sc-365503. MOLT-4 (A), Ramos (B), Hep G2 (C), SK-BR-3 (D), HL-60 (E) 和 K-562 (F) 全细胞裂解液中 EphA4 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

EphA4 抗体 (D-4): sc-365503

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寫評論提問

说明书
  • EphA4 抗体 D-4 是小鼠单克隆 IgG2b κ，EphA4抗体, 在14篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的EphA4的C-terminus附近的氨基酸889-921之间
  • EphA4 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 EphA4; 也和以下物种反应，包括： 和 canine and porcine
  • 抗EphA4抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EphA4 (D-4): sc-365503的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是EphA4 Antibody (D-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与EphA4 Antibody (D-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Eph抗体，包括EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphB1, EphB2, EphB3, EphB4和EphB6

EphA4抗体（D-4）是一种IgG2b κ小鼠单克隆EphA4抗体（也被指定为Ephrin A型受体4抗体、EPHA4抗体、SEK抗体或TYRO1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的EphA4蛋白。EphA4抗体（D-4）既可作为非共轭抗EphA4抗体形式提供，也可作为多种共轭形式的抗EphA4抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Eph亚家族是目前已鉴定的最大的受体蛋白酪氨酸激酶组。虽然这些受体的生物活性尚未确定，但越来越多的证据表明，它们参与中枢神经系统功能和发育。人类来源的Eph亚家族受体（及其鼠类/鸟类同源物）包括EphA1（Eph）、EphA2（Eck）、EphA3（Hek4）、EphA4（Hek8）、EphA5（Hek7）、EphA6（Hek12）、EphA7（Hek11/MDK1）、EphA8（Hek3）、EphB1（Hek6）、EphB2（Hek5）、EphB3（Cek10、Hek2）、EphB4（Htk）、EphB5（Hek9）和EphB6（Mep）。Eph受体的配体包括与EphA3和EphB1结合的ephrin-A4（LERK-4）。此外，ephrin-A2（ELF-1）被描述为EphA4的配体，ephrin-A3（Ehk1-L）作为EphA5的配体，ephrin-B2（Htk-L）作为EphB4（Htk）的配体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EphA4 抗体 (D-4) 参考文献:

  1. EphA4信号在辐射诱导结直肠癌细胞EMT样表型中的作用  |  de Marcondes, PG. and Morgado-Díaz, JA. 2017. J Cell Biochem. 118: 442-445. PMID: 27632701
  2. EphA4 通过促进星形胶质细胞过度活化阻碍脊髓神经元再生  |  Chen, X., et al. 2022. Cell Mol Neurobiol. 42: 1557-1568. PMID: 33595805
  3. EphA4在介导MND运动神经元死亡中的作用  |  Zhao, J., et al. 2021. Int J Mol Sci. 22: PMID: 34502339
  4. 在实验性青光眼模型中，EphA4/ephrinA3反向信号介导了Müller细胞中谷氨酸转运体GLAST的下调。  |  Zhou, ZX., et al. 2023. Glia. 71: 720-741. PMID: 36416239
  5. MicroRNA-204-5p 通过 EphA4/PI3K/AKT 信号通路改善缺血性脑卒中的神经损伤  |  Shi, CS., et al. 2023. ACS Chem Neurosci. 14: 2060-2073. PMID: 37196241
  6. ELF-1的鉴定和克隆，ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
  7. EPH相关受体蛋白酪氨酸激酶Htk配体的分子克隆。  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
  8. 鉴定 Eph 受体酪氨酸激酶家族新成员 Ehk-3 的全长和截短形式。  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
  9. 鉴定编码 MDK1（一种在小鼠神经系统中表达的新型受体酪氨酸激酶）变体的替代剪接 mRNA。  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
  10. 受体酪氨酸激酶 hek 和 elk 的配体：分离编码蛋白质家族的 cDNA。  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
  11. 五种人类 EPH 样受体蛋白-酪氨酸激酶的 cDNA 克隆和组织分布。  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
  12. eph相关酪氨酸激酶受体配体家族的分子特征。  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404

订购信息

产品名称产品编号规格价格数量收藏夹

EphA4 抗体 (D-4)

sc-365503
200 µg/ml
$316.00

EphA4 (D-4): m-IgGκ BP-HRP 套装

sc-522260
200 µg Ab, 40 µg BP
$354.00

EphA4 (D-4): m-IgG2b BP-HRP 套装

sc-549017
200 µg Ab; 10 µg BP
$354.00

EphA4 抗体 (D-4) AC

sc-365503 AC
500 µg/ml, 25% agarose
$416.00

EphA4 抗体 (D-4) HRP

sc-365503 HRP
200 µg/ml
$316.00

EphA4 抗体 (D-4) FITC

sc-365503 FITC
200 µg/ml
$330.00

EphA4 抗体 (D-4) PE

sc-365503 PE
200 µg/ml
$343.00

EphA4 抗体 (D-4) Alexa Fluor® 488

sc-365503 AF488
200 µg/ml
$357.00

EphA4 抗体 (D-4) Alexa Fluor® 546

sc-365503 AF546
200 µg/ml
$357.00

EphA4 抗体 (D-4) Alexa Fluor® 594

sc-365503 AF594
200 µg/ml
$357.00

EphA4 抗体 (D-4) Alexa Fluor® 647

sc-365503 AF647
200 µg/ml
$357.00

EphA4 抗体 (D-4) Alexa Fluor® 680

sc-365503 AF680
200 µg/ml
$357.00

EphA4 抗体 (D-4) Alexa Fluor® 790

sc-365503 AF790
200 µg/ml
$357.00

EphA4 (D-4) 中和勝肽

sc-365503 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365503 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody!This antibody works extremely well for WB on mouse cells.
Date published: 2019-08-19
Rated 1 out of 5 by from non-specificTest IF with this antibody on both embryonic and early postnatal mouse brain tissue. No specific signal could be detected.
Date published: 2019-03-15
Rated 1 out of 5 by from Not specific.Test IF with this antibody on both embryonic and early postnatal mouse brain tissue. No specific signal could be detected.
Date published: 2019-03-15
Rated 4 out of 5 by from Good immunoblots in human oral epithelial cellsI tested this EphA4 antibody (free sample) in immuno blots. Our samples were human oral epithelial cells. The membrane was incubated with 1:1000 Ab in TBST-5% BSA at 4C for O/N. One band was detected at ~125 kDa. This antibody worked fine for us.
Date published: 2017-07-25
Rated 1 out of 5 by from This antibody does NOT work in our Western blotWe tested this antibody (free sample from SC) in Western Blot (WB) multiple times. Unfortunately, it does NOT work. Our samples were from mouse neuronal stem cells, which have been confirmed containing EphA4 in both IHC and WB by using other Abs. In our experiment, each lane was loaded by 100 ug total protein. The membrane was incubated with 1:1000 Ab in TBST-5% BSA at 4C for O/N. According to our results, there were multiple bands > 150 kDa , but no band at 110-120 kDa which is the expected Mw of EphA4.
Date published: 2016-12-20
Rated 4 out of 5 by from Potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~120 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three major cross-reactive proteins of ~32, 48 and 62 kDa were also detected.
Date published: 2016-11-10
Rated 5 out of 5 by from Good publishable data in IF in chicken retinaGood publishable data in IF in chicken retina tissue. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from Produced excellent immunofluorescence membraneProduced excellent immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-05
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EphA4 抗体 (D-4) is rated 3.4 out of 5 by 9.
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