EPAS-1抗体 (190b) 推荐用于 mouse, rat 和 human 来源的EPAS-1 WB, IP, IF, IHC(P) 和 ELISA检测

EPAS-1 抗体 (190b): sc-13596

EPAS-1抗体 (190b) is rated 4.0 out of 5 by 9.
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说明书

    • 抗-EPAS-1 抗体 190b 是小鼠单克隆 IgG1 (kappa轻链),EPAS-1抗体,规格为200 µg/ml
    • 免疫human物种的重组EPAS-1
    • 抗-EPAS-1 抗体 (190b) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 EPAS-1
    • 抗-EPAS-1 抗体 (190b) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13596 X, 200 µg/0.1 ml)
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取EPAS-1 (190b): sc-13596免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What is recommended incubation time for western blot of cell extracts with sc-13596? Thank You.

Asked by: KateDeck
Thank you for your question. We recommend incubating the primary antibody for 1 hour at room temperature (or overnight at 4C) in 5% nonfat milk in TBST.
Answered by: Technical Support
Date published: 2019-08-17

When should the TransCruz reagent sc-13596 X be used instead of the standard form sc-13596? What is the difference between the two forms?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration. The TransCruz reagent is provided as 10x more concentrated than the regular form: sc-13596 X, 200 µg/0.1 ml.
Answered by: Technical Support 9
Date published: 2017-01-12

Can this antibody be used for ChIP?

Asked by: TinTin
Thank you for your question! Yes, this antibody is recommeded for IP, which includes ChIP and CoIP. For more information, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Technical Support 9
Date published: 2017-01-12
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Rated 4 out of 5 by from 良いです。 内在性タンパク質を検出できますが、そのためには1次抗体をCanget signalで希釈する必要があります。
Date published: 2020-10-20
Rated 1 out of 5 by from 在小鼠肾脏组织中没有杂到目标条带。 这抗体在肾脏组织中没有杂到目标条带,在70KD以上都没有任何条带,但在50~30KD之间有许多杂带,。
Date published: 2017-11-27
Rated 5 out of 5 by from Great I am happy to get chance to try it. Will let you know how it is in rat sample.
Date published: 2017-07-05
Rated 4 out of 5 by from good band. Band had seen in hypoxic condition at 1:200 dilution in western blot.
Date published: 2017-06-16
Rated 4 out of 5 by from Buon funzionamento Sebbene scettica ho provato che questo anticorpo funziona
Date published: 2017-03-22
Rated 5 out of 5 by from Strong western data Very clear western blot data; strong signal with low background
Date published: 2017-01-31
Rated 5 out of 5 by from Good choice for WB Whether your samples are mouse, rat or human, sc-13596 will provide you with strong and clear bands. This antibody has been published nearly 20 times.
Date published: 2017-01-30
Rated 4 out of 5 by from Strong band seen in WB using CoCl2 Strong band seen in WB using CoCl2-induced HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-10
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