Date published: 2026-10-24
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Enolase Antibody (D-8) - Western Blotting - Image 287576
烯醇化酶抗体 (D-8): sc-390163. Hep G2 (A) 和 HeLa (B) 全细胞裂解液中 Enolase 表达的 Western 印迹分析.
烯醇化酶抗体 (D-8): sc-390163. Hep G2 (A) 和 HeLa (B) 全细胞裂解液中 Enolase 表达的 Western 印迹分析.

Enolase 抗体 (D-8): sc-390163

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说明书
  • Enolase抗体(D-8)是小鼠单克隆IgG1 κ, 在1篇文献中引用，规格为200 µg/ml
  • 针对Enolase的human起源的内部区域内的肽图
  • 推荐用于 mouse, rat 和 human 来源的α Enolase and β Enolase WB, IP, IF 和 ELISA检测
  • 看 Enolase (A-5): sc-271384，可查看有标记的 Enolase 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Enolase Antibody (D-8) for WB applications. 的首选辅助检测试剂，这些试剂现与Enolase Antibody (D-8) 打包提供（请参阅下面的订购信息）。
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関連項目

附加Enolase抗体，包括α Enolase, β Enolase和γ Enolase

烯醇化酶已被鉴定为高度保守的细胞质糖酵解酶，可能参与分化过程。已确定存在三种同工酶：α-烯醇化酶、β-烯醇化酶和γ-烯醇化酶。α-烯醇化酶的表达已在大多数组织中检测到，而β-烯醇化酶主要在肌肉组织中表达，γ-烯醇化酶仅在神经组织中检测到。这些同工酶以同二聚体和异二聚体形式存在，并在糖酵解途径中将磷酸甘油酸转化为磷酸烯醇丙酮酸中发挥作用。这种由433个氨基酸组成的蛋白质与酵母烯醇化酶具有67%的同源性，与大鼠非神经烯醇化酶具有94%的同源性。研究还表明，α-烯醇化酶由与编码τ-晶状体蛋白（一种晶状体结构蛋白）的相同基因编码。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Enolase 抗体 (D-8) 参考文献:

  1. 肌形成过程中烯醇化酶同种型与微管蛋白和微管的相互作用。  |  Keller, A., et al. 2007. Biochim Biophys Acta. 1770: 919-26. PMID: 17368730
  2. 烯醇化酶在乳腺癌细胞系亚群中的定位。  |  Seweryn, E., et al. 2009. Z Naturforsch C J Biosci. 64: 754-8. PMID: 19957447
  3. 滑膜支原体烯醇化酶是一种纤溶酶原/纤维蛋白结合蛋白。  |  Bao, S., et al. 2014. BMC Vet Res. 10: 223. PMID: 25253294
  4. 烯醇化酶存在于3T3细胞质液相中。  |  Pagliaro, L., et al. 1989. J Cell Sci. 94 (Pt 2): 333-42. PMID: 2621228
  5. 烯醇化酶1刺激糖酵解，促进胃癌的耐药性。  |  Qian, X., et al. 2017. Oncotarget. 8: 47691-47708. PMID: 28548950
  6. 隐孢子虫烯醇化酶的免疫定位和酶活性分析。  |  Mi, R., et al. 2017. Parasit Vectors. 10: 273. PMID: 28569179
  7. 神经元特异性烯醇化酶作为免疫组织化学标记物比其名声更佳。  |  Mjønes, P., et al. 2017. J Histochem Cytochem. 65: 687-703. PMID: 28972818
  8. 幽门螺杆菌烯醇化酶的生物化学和生物物理特性。  |  López-López, MJ., et al. 2018. Biomed Res Int. 2018: 9538193. PMID: 30648111
  9. 克雷伯氏肺炎烯醇化酶样膜蛋白与人类纤溶酶原相互作用。  |  Serek, P., et al. 2021. Int J Med Microbiol. 311: 151518. PMID: 34237624
  10. 丝状蓝藻烯醇化酶的结构揭示了磷酸烯醇丙酮酸结合的关键方面。  |  González, JM., et al. 2022. Acta Crystallogr F Struct Biol Commun. 78: 177-184. PMID: 35400670

订购信息

产品名称产品编号规格价格数量收藏夹

Enolase 抗体 (D-8)

sc-390163
200 µg/ml
$322.00

Enolase (D-8): m-IgG Fc BP-HRP 套装

sc-540765
200 µg Ab; 10 µg BP
$361.00

Enolase (D-8): m-IgG1 BP-HRP 套装

sc-542357
200 µg Ab; 20 µg BP
$361.00

Enolase (D-8) 中和勝肽

sc-390163 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-390163 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by MS from Strong band in WB in HepG2 and HeLa wholeStrong band in WB in HepG2 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-31
Rated 5 out of 5 by NZ from Robust signal in WB in HepG2 and HeLa wholeRobust signal in WB in HepG2 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by LF from Good specificity in WB in A549 whole cell lysateGood specificity in WB in A549 whole cell lysate. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by LF from WB publishable data with human serum samplesWB publishable data with human serum samples - SCBT Publication Review
Date published: 2014-03-05
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Enolase 抗体 (D-8) is rated 5.0 out of 5 by 4.
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