Enigma Antibody (D-10): sc-376359
- Enigma Antibody (D-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 13-47 near the N-terminus of Enigma of human origin
- recommended for detection of Enigma of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Enigma Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Enigma Antibody (D-10) (see ordering information below).
QUICK LINKS
SEE ALSO...
Enigma Antibody (D-10) is a mouse monoclonal IgG1 antibody that detects Enigma in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Enigma, also known as PDLIM7 (PDZ and LIM domain 7) or LMP1 (LIM mineralization protein), is a 457 amino acid protein that plays a crucial role in cellular scaffolding and protein assembly, particularly in skeletal muscle and other tissues. Enigma′s unique structure features three LIM zinc-binding domains and one PDZ domain, which facilitate function as an adaptor protein that links various signaling molecules to actin filaments. This interaction is vital for maintaining cytoskeleton integrity and enabling effective cellular communication. Enigma (D-10) antibody recognizes a protein implicated in bone formation processes, including both direct and endochondral ossification, highlighting importance in skeletal development and repair mechanisms. Six isoforms of Enigma, resulting from alternative splicing, further underscore complexity in different biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Enigma Antibody (D-10) References:
- The PDZ domain of the LIM protein enigma binds to beta-tropomyosin. | Guy, PM., et al. 1999. Mol Biol Cell. 10: 1973-84. PMID: 10359609
- Differential interaction of Enigma protein with the two RET isoforms. | Borrello, MG., et al. 2002. Biochem Biophys Res Commun. 296: 515-22. PMID: 12176011
- Scaffold protein enigma homolog activates CREB whereas a short splice variant prevents CREB activation in cardiomyocytes. | Ito, J., et al. 2015. Cell Signal. 27: 2425-33. PMID: 26365342
- PDLIM7 is a novel target of the ubiquitin ligase Nedd4-1 in skeletal muscle. | D'Cruz, R., et al. 2016. Biochem J. 473: 267-76. PMID: 26556890
- Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 2. | Nakatani, M., et al. 2016. Biochem Biophys Res Commun. 474: 413-420. PMID: 27114303
- TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1. | Wu, Z., et al. 2023. Cell Biosci. 13: 39. PMID: 36823643
- H3K27 acetylation activated-PDLIM7 promotes castration-resistant prostate cancer progression by inducing O-Glycosylation of YAP1 protein. | Liu, Q., et al. 2024. Transl Oncol. 40: 101830. PMID: 38056280
- Regulatory and Interacting Partners of PDLIM7 in Thyroid Cancer. | Rood, K., et al. 2023. Curr Oncol. 30: 10450-10462. PMID: 38132395
- PDLIM7 Promotes Tumor Metastasis in Papillary Thyroid Carcinoma via Stabilizing Focal Adhesion Kinase Protein. | Yang, F., et al. 2024. Thyroid. 34: 598-610. PMID: 38243825
- OGA mutant aberrantly hydrolyzes O-GlcNAc modification from PDLIM7 to modulate p53 and cytoskeleton in promoting cancer cell malignancy. | Hu, CW., et al. 2024. Proc Natl Acad Sci U S A. 121: e2320867121. PMID: 38838015
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Enigma Antibody (D-10) | sc-376359 | 200 µg/ml | $322.00 | |||
Enigma Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-540553 | 200 µg Ab; 10 µg BP | $361.00 | |||
Enigma Antibody (D-10): m-IgG1 BP-HRP Bundle | sc-542201 | 200 µg Ab; 20 µg BP | $361.00 | |||
Enigma (D-10) Neutralizing Peptide | sc-376359 P | 100 µg/0.5 ml | $69.00 |