Date published: 2025-9-7

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Endophilin B1 抗体 (G-6): sc-374146

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说明书
  • Endophilin B1 抗体 G-6 是小鼠单克隆 IgG1 κ,Endophilin B1抗体, 在2篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Endophilin B1的N-terminus附近的氨基酸61-91之间
  • Endophilin B1 抗体 (G-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Endophilin B1; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗Endophilin B1抗体(G-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Endophilin B1 (G-6): sc-374146免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Endophilin B1 抗体 (G-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

関連項目

Endophilin B1抗体(G-6)是一种IgG1 κ小鼠单克隆Endophilin B1抗体(也称为SH3GLB1抗体或Bif-1抗体),它能够通过蛋白质印迹法、免疫沉淀法、免疫荧光法、免疫组织化学法(IHC-P)和酶联免疫吸附试验等多种方法,有效地检测小鼠、大鼠和人类来源的Endophilin B1蛋白。此外,Endophilin B1抗体(G-6)不仅提供非偶联抗Endophilin B1抗体的形式,还提供多种偶联形式的抗Endophilin B1抗体,这些偶联形式包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。内噬蛋白(endophilins)构成了一个与Amphiphysin、Synaptojanin和Dynamin相关的蛋白质家族,并在神经终端的突触囊泡转运过程中发挥着重要作用。这些内噬蛋白的表达模式与它们在神经元突触中的细胞功能紧密相关。特别地,Endophilin B1作为内噬蛋白家族B亚组的成员,对于维持线粒体形态以及调节线粒体外膜的动态变化至关重要。其N端结构域与A类内噬蛋白的脂质结合和修饰(LBM)结构域展现出了高度的相似性。值得一提的是,Endophilin B1基因至少编码了三种剪接变体,分别是广泛分布于各种组织的Endophilin B1a,以及似乎是脑特异的Endophilin B1b和B1c。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Endophilin B1 抗体 (G-6) 参考文献:

  1. SH3GLB,一个具有 SH3 结构域的新的嗜内蛋白相关蛋白家族。  |  Pierrat, B., et al. 2001. Genomics. 71: 222-34. PMID: 11161816
  2. 嗜内酯蛋白 B1b 的特征,它是一种脑特异性膜相关溶血磷脂酸酰基转移酶,具有不同于嗜内酯蛋白 A1 的特性。  |  Modregger, J., et al. 2003. J Biol Chem. 278: 4160-7. PMID: 12456676
  3. 唐氏综合征胎儿大脑皮层蛋白质表达异常。  |  Engidawork, E., et al. 2003. Neuroscience. 122: 145-54. PMID: 14596856
  4. 嗜内皮蛋白与莫洛尼小鼠白血病病毒 Gag 相互作用并调节病毒的产生。  |  Wang, MQ., et al. 2003. J Biol. 3: 4. PMID: 14659004
  5. 线粒体形态的维持需要嗜内酯蛋白 B1。  |  Karbowski, M., et al. 2004. J Cell Biol. 166: 1027-39. PMID: 15452144
  6. 转移相关蛋白 1 与嗜内蛋白 3 之间的直接相互作用。  |  Aramaki, Y., et al. 2005. FEBS Lett. 579: 3731-6. PMID: 15978591
  7. Bif-1 的缺失会抑制 Bax/Bak 的构象变化和线粒体凋亡。  |  Takahashi, Y., et al. 2005. Mol Cell Biol. 25: 9369-82. PMID: 16227588

订购信息

产品名称产品编号规格价格数量收藏夹

Endophilin B1 抗体 (G-6)

sc-374146
200 µg/ml
$316.00

Endophilin B1 抗体 (G-6) AC

sc-374146 AC
500 µg/ml, 25% agarose
$416.00

Endophilin B1 抗体 (G-6) HRP

sc-374146 HRP
200 µg/ml
$316.00

Endophilin B1 抗体 (G-6) FITC

sc-374146 FITC
200 µg/ml
$330.00

Endophilin B1 抗体 (G-6) PE

sc-374146 PE
200 µg/ml
$343.00

Endophilin B1 抗体 (G-6) Alexa Fluor® 488

sc-374146 AF488
200 µg/ml
$357.00

Endophilin B1 抗体 (G-6) Alexa Fluor® 546

sc-374146 AF546
200 µg/ml
$357.00

Endophilin B1 抗体 (G-6) Alexa Fluor® 594

sc-374146 AF594
200 µg/ml
$357.00

Endophilin B1 抗体 (G-6) Alexa Fluor® 647

sc-374146 AF647
200 µg/ml
$357.00

Endophilin B1 抗体 (G-6) Alexa Fluor® 680

sc-374146 AF680
200 µg/ml
$357.00

Endophilin B1 抗体 (G-6) Alexa Fluor® 790

sc-374146 AF790
200 µg/ml
$357.00

Endophilin B1 (G-6) 中和勝肽

sc-374146 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-374146 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Caco-2 cells中WB和IF验证结果很好!Western blot and IF analysis of Endophilin B1 expression in the human coloncarcinoma cell line Caco-2 cells were carried out. The fluorescence was observed in Caco-2 cells. A band between 35 kd – 55 kd was detected in the cell lysates.
Date published: 2017-10-10
Rated 5 out of 5 by from the function of this antibody is very good.I applied this antibody a month ago, and I used it on WB. The cell of my experiment is the vascular smooth muscle from SD RAT. I can see the clear change after drug. But this antibody just can be used once a time. We can not repeat the resule.
Date published: 2017-01-18
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic stainingSuperb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-19
Rated 4 out of 5 by from Nice bands seen in WB using HeLa and HepNice bands seen in WB using HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-25
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Endophilin B1 抗体 (G-6) is rated 4.6 out of 5 by 5.
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