Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
endobrevin Antibody (G-12) - Western Blotting - Image 359066
endobrevin 抗体 (G-12): sc-166820. HeLa (A), HEK293 (B), A-431 (C), U-937 (D) 和 HL-60 (E) 全细胞裂解液中内切酶表达的 Western 印迹分析.
endobrevin 抗体 (G-12): sc-166820. HeLa (A), HEK293 (B), A-431 (C), U-937 (D) 和 HL-60 (E) 全细胞裂解液中内切酶表达的 Western 印迹分析.

endobrevin 抗体 (G-12): sc-166820

4.9(9)
寫評論提問

说明书
  • endobrevin 抗体 G-12 是小鼠单克隆 IgG1 κ，endobrevin抗体, 在17篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的endobrevin的N-terminus附近的氨基酸28-47之间
  • endobrevin 抗体 (G-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 endobrevin
  • 抗endobrevin抗体（G-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取endobrevin (G-12): sc-166820的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是endobrevin Antibody (G-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与endobrevin Antibody (G-12) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

Endobrevin抗体（G-12）是一种IgG1 κ小鼠单克隆Endobrevin抗体（也称为Endobrevin抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人源的Endobrevin蛋白。Endobrevin抗体（G-12）既有非偶联抗Endobrevin抗体形式，也有多种偶联形式的抗Endobrevin抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。Syntaxins最初被认为是停靠蛋白，但最近被归类为锚定蛋白，它们将自己锚定在细胞膜的细胞质表面。Syntaxins与参与胞吐作用的各种蛋白质结合，包括VAMPs（囊泡相关膜蛋白）、NSF（N-乙基马来酰亚胺敏感因子）、SNAPs（可溶性NSF附着蛋白）和突触结合蛋白。Endobrevin，也被称为VAMP-8或ED，是一种100个氨基酸的单通道IV型膜蛋白，属于synaptobrevin家族。Endobrevin在序列上与synaptobrevins相似，在肾脏中大量表达，在心脏和脾脏中中度表达，在脑、胸腺和肝脏中略有表达。Endobrevin通过含有Endobrevin的SNARE复合物与SNAPs特异性相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

endobrevin 抗体 (G-12) 参考文献:

  1. 哺乳动物细胞分裂末期需要Syntaxin 2和endobrevin。  |  Low, SH., et al. 2003. Dev Cell. 4: 753-9. PMID: 12737809
  2. Endobrevin/VAMP-8是血小板释放反应的主要v-SNARE。  |  Ren, Q., et al. 2007. Mol Biol Cell. 18: 24-33. PMID: 17065550
  3. Endobrevin/VAMP-8依赖的致密颗粒释放可促进体内血栓形成。  |  Graham, GJ., et al. 2009. Blood. 114: 1083-90. PMID: 19395672
  4. 囊泡转运受体的 syntaxin 家族。  |  Bennett, MK., et al. 1993. Cell. 74: 863-73. PMID: 7690687
  5. 突触珠蛋白与突触素的结合：控制外吞融合机的潜在机制  |  Edelmann, L., et al. 1995. EMBO J. 14: 224-31. PMID: 7835333
  6. 由突触珠蛋白, 语法蛋白和 SNAP25 组成的突触核心复合体形成了高亲和力的 alpha-SNAP 结合位点。  |  McMahon, HT. and Südhof, TC. 1995. J Biol Chem. 270: 2213-7. PMID: 7836452
  7. 神经系统中与蛋白质有关的外排。  |  Yamaguchi, K. and Akagawa, K. 1994. Neurosci Res. 20: 289-92. PMID: 7870381
  8. 突触小泡膜融合复合体：梭菌类神经毒素对组装的作用。  |  Hayashi, T., et al. 1994. EMBO J. 13: 5051-61. PMID: 7957071
  9. 突触标记蛋白（p65）在调节外吞中的作用  |  Elferink, LA., et al. 1993. Cell. 72: 153-9. PMID: 8422678
  10. α-SNAP对NSF ATP酶活性的刺激是SNARE复合物解体和外吞所必需的。  |  Barnard, RJ., et al. 1997. J Cell Biol. 139: 875-83. PMID: 9362506
  11. 突触外泌核心复合体的结构组织。  |  Lin, RC. and Scheller, RH. 1997. Neuron. 19: 1087-94. PMID: 9390521
  12. Endobrevin是一种新型的突触素/VAMP样蛋白，优先与早期内体结合。  |  Wong, SH., et al. 1998. Mol Biol Cell. 9: 1549-63. PMID: 9614193

订购信息

产品名称产品编号规格价格数量收藏夹

endobrevin 抗体 (G-12)

sc-166820
200 µg/ml
$316.00

endobrevin (G-12): m-IgG Fc BP-HRP 套装

sc-529058
200 µg Ab; 10 µg BP
$354.00

endobrevin (G-12): m-IgGκ BP-HRP 套装

sc-521757
200 µg Ab, 40 µg BP
$354.00

endobrevin (G-12): m-IgG1 BP-HRP 套装

sc-543294
200 µg Ab; 20 µg BP
$354.00

endobrevin 抗体 (G-12) AC

sc-166820 AC
500 µg/ml, 25% agarose
$416.00

endobrevin 抗体 (G-12) HRP

sc-166820 HRP
200 µg/ml
$316.00

endobrevin 抗体 (G-12) FITC

sc-166820 FITC
200 µg/ml
$330.00

endobrevin 抗体 (G-12) PE

sc-166820 PE
200 µg/ml
$343.00

endobrevin 抗体 (G-12) Alexa Fluor® 488

sc-166820 AF488
200 µg/ml
$357.00

endobrevin 抗体 (G-12) Alexa Fluor® 546

sc-166820 AF546
200 µg/ml
$357.00

endobrevin 抗体 (G-12) Alexa Fluor® 594

sc-166820 AF594
200 µg/ml
$357.00

endobrevin 抗体 (G-12) Alexa Fluor® 647

sc-166820 AF647
200 µg/ml
$357.00

endobrevin 抗体 (G-12) Alexa Fluor® 680

sc-166820 AF680
200 µg/ml
$357.00

endobrevin 抗体 (G-12) Alexa Fluor® 790

sc-166820 AF790
200 µg/ml
$357.00

endobrevin (G-12) 中和勝肽

sc-166820 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-166820 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_166820, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 100ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent in WBWB in cell lysate of MDA dilution 1:1000 Clear and well detection of a band at the correct molecular weight
Date published: 2019-04-24
Rated 5 out of 5 by from great antibody for WBI got specifical band in HEK 293T cells, its ok for WB
Date published: 2017-06-21
Rated 4 out of 5 by from Great for WBgreat antibody for WB in U87 cell whole lysates, only one band! Excellent!
Date published: 2017-02-13
Rated 5 out of 5 by from excellent for WBOnly one specific WB band appears at 11kd in human samples.
Date published: 2017-02-08
Rated 5 out of 5 by from Excellent antibodyThe endobrevin Antibody (G-12): sc-166820 works very well for immunofluorescence, showing cytoplasmic localized staining pattern.
Date published: 2017-01-18
Rated 5 out of 5 by from GReat for WBDetects a very strong and specific band in RBL-2H3 mast cell lysate.
Date published: 2016-11-29
Rated 5 out of 5 by from Used this antibody in IF and got correctUsed this antibody in IF and got correct staining pattern.
Date published: 2015-08-11
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-07
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_166820, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
endobrevin 抗体 (G-12) is rated 4.9 out of 5 by 9.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_166820, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 109ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。