Electrophoresis Sample Buffer, 2X A ready-to-use reducing sample buffer for SDS-PAGE.

Electrophoresis Sample Buffer, 2X

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应用; A ready-to-use reducing sample buffer for SDS-PAGE
* 参考分析证明 大量特定数据 (包括水 含量).
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Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included as a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer contains DTT and TCEP-HCl, which disrupt disulfide bonds to ensure proteins are fully denatured before loading on the gel.
Contains: glycerol (sc-29095), TCEP-HCl (sc-203290), DTT (sc-29089), SDS (sc-24950), MES (sc-255262), EDTA (sc-286969), Triton X-100 (sc-29112) and bromophenol blue (sc-24971).
For non-reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (non-reducing) (sc-45085) is recommended.


参考文献

1. Toyoda, E., et al. 2005. Pancreas. 30: 168-173. PMID: 15714139
2. Heinz, L.X., et al. 2006. Blood. 107: 1445-1453. PMID: 16223775
3. Gohil, K., et al. 2008. Brain Res. 1201: 167-176. PMID: 18299118
4. Popovici, R.M., et al. 2008. Endocrinology. 149: 1136-1143. PMID: 18048494
5. Tang, Y., et al. 2010. J. Nutr. Biochem. 21: 237-246. PMID: 19369054

用法 :
Mix sample with an equal volume of Electrophoresis Sample Buffer, 2X. Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
外观 :
Aqueous solution
物理状态 :
Liquid
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。

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