EGF抗体 (F-9) 推荐用于 human 来源的precursor and mature EGF WB, IP, IF 和 ELISA检测

EGF 抗体 (F-9): sc-166779

EGF抗体 (F-9) is rated 4.3 out of 5 by 6.
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说明书

    • 抗-EGF 抗体 F-9 是小鼠单克隆 IgG1 κ,EGF抗体, 在6篇文献中引用,规格为200 µg/ml
    • raised against amino acids 971-1023 representing mature EGF of human origin
    • EGF 抗体 (F-9) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 precursor and mature EGF
    • 抗-EGF 抗体 (F-9) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取EGF (F-9): sc-166779免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 EGF Antibody (F-9) for WB首选应用二抗。这些试剂现在以套装形式提供EGF Antibody (F-9) (见下方订购信息).
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Can EGF (F-9): sc-166779 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, EGF (F-9): sc-166779 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from questionable quality I tried this antibody, but I had to run it at an extremely high concentration in the trial format in order to see any kind of band.
Date published: 2018-01-22
Rated 4 out of 5 by from great for WB A nice antibody for Weston Blot.I chose dilution of 1:300,and I got a good result.
Date published: 2017-07-11
Rated 5 out of 5 by from Antibody is really great. Antibody is really great,can be used in many cell lines.
Date published: 2017-04-28
Rated 5 out of 5 by from Produced publishable IF data This EGF monoclonal antibody was referenced in the literature for the use of IF on HiCHO cells.
Date published: 2017-01-23
Rated 5 out of 5 by from Excellent result Excellent Western blot data of human recombinant EGF protein, with specific band and even no background.
Date published: 2016-11-22
Rated 5 out of 5 by from Good Western blot data of EGF expression Good Western blot data of EGF expression in human platelet extract. -SCBT QC
Date published: 2014-07-13
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