Date published: 2025-9-5

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EFP 抗体 (E-4): sc-166926

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说明书
  • EFP 抗体 E-4 是小鼠单克隆 IgG2b κ,EFP抗体, 在8篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的EFP的N-terminus附近的氨基酸158-173之间
  • EFP 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 EFP
  • 抗EFP抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-166926 X, 200 µg/0.1 ml)
  • 关于如何获取EFP (E-4): sc-166926免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是EFP 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与EFP 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

EFP抗体(E-4)是一种IgG2b κ小鼠单克隆EFP抗体(也称为EFP抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的EFP蛋白。EFP抗体(E-4)既可以以非偶联的抗EFP抗体形式提供,也可以以多种偶联形式的抗EFP抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。EFP(雌激素反应指蛋白)是一种转录因子,其含量受雌激素调节。它已被鉴定为RING指蛋白家族的成员,这是一类含有Zn2+结合域的蛋白质,被称为C3HC4或RING指结构域。EFP还含有两个B盒结构域和一个卷曲螺旋区域(一个反式激活结构域),这是RING指蛋白家族中一个亚组的特征。雌激素调节多种组织中靶细胞的生长、分化和功能;然而,很少有基因被证明是直接受雌激素调节的。据推测,EFP可能在信号级联中介导雌激素活性,其中雌激素-ER结合到EFP基因下游的雌激素反应元件(ERE)上,上调EFP基因的表达。然后,EFP基因产物可能激活次级雌激素反应基因的转录。进一步的研究表明,EFP启动子可能受多个元件及其相互作用因子的调节。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EFP 抗体 (E-4) 参考文献:

  1. Efp 靶向 14-3-3 sigma 进行蛋白分解,促进乳腺肿瘤生长。  |  Urano, T., et al. 2002. Nature. 417: 871-5. PMID: 12075357
  2. 一种泛素 E3 连接酶 Efp 受干扰素上调,并与 ISG15 连接。  |  Nakasato, N., et al. 2006. Biochem Biophys Res Commun. 351: 540-6. PMID: 17069755
  3. 雌激素受体α的配体依赖性转录是由泛素连接酶EFP介导的。  |  Nakajima, A., et al. 2007. Biochem Biophys Res Commun. 357: 245-51. PMID: 17418098
  4. 核受体加深了我们对转录调控的了解。  |  Green, S. and Chambon, P. 1988. Trends Genet. 4: 309-14. PMID: 2853466
  5. 类固醇和甲状腺激素受体超家族。  |  Evans, RM. 1988. Science. 240: 889-95. PMID: 3283939
  6. 小鼠雌激素反应性手指蛋白 Efp 的分子克隆, 结构和表达。在靶器官中与雌激素受体 mRNA 共同定位。  |  Orimo, A., et al. 1995. J Biol Chem. 270: 24406-13. PMID: 7592654
  7. 急性早幼粒细胞白血病原癌基因 PML 的 RING 手指结构域的溶液结构。  |  Borden, KL., et al. 1995. EMBO J. 14: 1532-41. PMID: 7729428
  8. 基因组结合位点克隆揭示了一种编码 RING 手指蛋白的雌激素反应基因。  |  Inoue, S., et al. 1993. Proc Natl Acad Sci U S A. 90: 11117-21. PMID: 8248217
  9. 人类雌激素反应性手指蛋白(efp)基因 5'-flanking 区域的多种调控元件和结合蛋白。  |  Ikeda, K., et al. 1997. Biochem Biophys Res Commun. 236: 765-71. PMID: 9245730

订购信息

产品名称产品编号规格价格数量收藏夹

EFP 抗体 (E-4)

sc-166926
200 µg/ml
$316.00

EFP (E-4): m-IgG2b BP-HRP 套装

sc-548684
200 µg Ab; 10 µg BP
$354.00

EFP 抗体 (E-4) X

sc-166926 X
200 µg/0.1 ml
$316.00

EFP 抗体 (E-4) AC

sc-166926 AC
500 µg/ml, 25% agarose
$416.00

EFP 抗体 (E-4) HRP

sc-166926 HRP
200 µg/ml
$316.00

EFP 抗体 (E-4) FITC

sc-166926 FITC
200 µg/ml
$330.00

EFP 抗体 (E-4) PE

sc-166926 PE
200 µg/ml
$343.00

EFP 抗体 (E-4) Alexa Fluor® 488

sc-166926 AF488
200 µg/ml
$357.00

EFP 抗体 (E-4) Alexa Fluor® 546

sc-166926 AF546
200 µg/ml
$357.00

EFP 抗体 (E-4) Alexa Fluor® 594

sc-166926 AF594
200 µg/ml
$357.00

EFP 抗体 (E-4) Alexa Fluor® 647

sc-166926 AF647
200 µg/ml
$357.00

EFP 抗体 (E-4) Alexa Fluor® 680

sc-166926 AF680
200 µg/ml
$357.00

EFP 抗体 (E-4) Alexa Fluor® 790

sc-166926 AF790
200 µg/ml
$357.00

EFP (E-4) 中和勝肽

sc-166926 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-166926 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Not sensitive for mouse cell lysateIt is not sensitive for mouse cell lysate, seeking another one
Date published: 2019-04-19
Rated 4 out of 5 by from Recommended for western blotting!It detected endogenous TRIM25 in immortalized HFFs (1:500 in 5% milk in TBS-0.1% Tween, overnight at 4C).
Date published: 2017-12-12
Rated 5 out of 5 by from Works with porcine cellsWorks with a porcine macrophage cell line for immunofluorescence.
Date published: 2017-09-30
Rated 5 out of 5 by from easyAntibody does not seem so easy to use, but overall it is still good, I think it can, recommend buying
Date published: 2017-01-17
Rated 3 out of 5 by from Not a perfect antibodyNot a perfect antibody, but we also had difficult samples with low amounts of TRIM25.
Date published: 2015-11-11
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-17
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EFP 抗体 (E-4) is rated 4.4 out of 5 by 7.
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