Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EF-1 α2 Double Nickase Plasmid (h): sc-401444-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EF-1 α2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EF-1 α2 Double Nickase Plasmid (h) and EF-1 α2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EEF1A2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-1 α2 Double Nickase Plasmid (h) | sc-401444-NIC | 20 µg | $410.00 | |||
EF-1 α2 Double Nickase Plasmid (h2) | sc-401444-NIC-2 | 20 µg | $410.00 |
EEF1A2 encodes eukaryotic translation elongation factor 1 alpha 2 (EF-1 α2), a GTP-binding protein that delivers aminoacyl-tRNAs to the ribosomal A site during mRNA translation elongation. EF-1 α2 contributes to translational control, proteostasis, and cellular stress responses, with additional links to cytoskeletal organization through actin-associated functions described for EF1A family members. In human tissues, EEF1A2 shows enriched expression in neurons and muscle and is important for maintaining high protein synthesis capacity in differentiated cells. Dysregulation or genetic alteration of EEF1A2 has been associated with neurodevelopmental phenotypes and cancer-related transcriptional programs, making it a useful node for studying translation-dependent remodeling of cell state.
EF-1 α2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EEF1A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EEF1A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EEF1A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EEF1A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.