Date published: 2025-9-7
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EAAT2 Antibody (E-1) - Western Blotting - Image 355827
EAAT2 抗体 (E-1): sc-365634. SH-SY5Y (A), U-87 MG (B) 和 HeLa (C) 全细胞裂解液以及人胎儿脑 (D) 和小鼠脑 (E) 组织提取物中 EAAT2 表达的 Western 印迹分析.
EAAT2 抗体 (E-1): sc-365634. SH-SY5Y (A), U-87 MG (B) 和 HeLa (C) 全细胞裂解液以及人胎儿脑 (D) 和小鼠脑 (E) 组织提取物中 EAAT2 表达的 Western 印迹分析.

EAAT2 抗体 (E-1): sc-365634

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说明书
  • EAAT2 抗体 E-1 是小鼠单克隆 IgG2b κ，EAAT2抗体, 在48篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-85位于human物种的EAAT2的N-terminus附近
  • EAAT2 抗体 (E-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 EAAT2
  • 抗EAAT2抗体（E-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EAAT2 (E-1): sc-365634的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是EAAT2 Antibody (E-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与EAAT2 Antibody (E-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外EAAT抗体，包括EAAT1, EAAT2和EAAT4

EAAT2抗体（E-1）是一种IgG2b κ型小鼠单克隆EAAT2抗体（也被称为兴奋性氨基酸转运体2抗体、EAAT-2抗体、溶质载体家族1成员2（SLC1A2）抗体或谷氨酸转运体1（GLT-1）抗体），可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）和酶联免疫吸附测定法（ELISA）检测来自小鼠、大鼠和人类的EAAT2蛋白。EAAT2抗体（E-1）既有非偶联形式的抗EAAT2抗体，也有多种偶联形式的抗EAAT2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。兴奋性氨基酸转运体1（EAAT1）是两种胶质谷氨酸转运体中的一种，可清除神经元信号传递过程中产生的细胞外谷氨酸。兴奋性氨基酸转运体（EAATs）是定位于胶质细胞和突触前谷氨酸能神经末梢的膜结合蛋白。EAATs转运兴奋性神经递质L-谷氨酸和D-天冬氨酸，这一过程对于终止谷氨酸的突触后作用是至关重要的。已证明EAAT蛋白对氨基酸神经递质的再摄取可以保护神经元免受由氨基酸神经递质积累引起的兴奋性毒性作用。在人类大脑中已发现三种谷氨酸转运体，分别命名为EAAT1-3。EAAT1和EAAT3也在各种非神经组织中表达，而EAAT2的表达似乎仅限于大脑。胶质谷氨酸转运体EAAT1的表面表达受胰岛素样生长因子1通过激活磷脂酰肌醇-3-激酶的刺激。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EAAT2 抗体 (E-1) 参考文献:

  1. 谷氨酸转运体兴奋性氨基酸转运体 1 (EAAT1) 在阿尔茨海默病中的异常表达。  |  Scott, HL., et al. 2002. J Neurosci. 22: RC206. PMID: 11826152
  2. 泛素连接酶 Nedd4-2 以及血清和糖皮质激素诱导激酶同工酶 SGK1/3 和蛋白激酶 B 对谷氨酸转运体 EAAT1 的调控。  |  Boehmer, C., et al. 2003. J Neurochem. 86: 1181-8. PMID: 12911626
  3. 人类兴奋性氨基酸转运体 1（EAAT1）的转录调控：EAAT1 启动子的克隆及其在人类星形胶质细胞中的基础活性和诱导活性的特征。  |  Kim, SY., et al. 2003. J Neurochem. 87: 1485-98. PMID: 14713304
  4. 咪达唑仑通过调节EAAT2，有助于保护新生大鼠免受缺氧/再氧合引起的脑损伤。  |  Tang, Z., et al. 2020. Brain Res Bull. 161: 136-146. PMID: 32433937
  5. EAAT2作为阿尔茨海默病治疗研究的目标：系统综述。  |  Wood, OWG., et al. 2022. Front Neurosci. 16: 952096. PMID: 36033606
  6. PS1/γ-分泌酶在阿尔茨海默病中充当谷氨酸转运蛋白EAAT2/GLT-1的流氓伴侣。  |  Perrin, F., et al. 2024. Acta Neuropathol Commun. 12: 166. PMID: 39434170
  7. 从人类运动皮层克隆的三种谷氨酸转运体亚型的功能比较。  |  Arriza, JL., et al. 1994. J Neurosci. 14: 5559-69. PMID: 7521911
  8. 小鼠兴奋性氨基酸转运体 EAAT2：分离, 表征以及与小鼠 2 号染色体上神经兴奋性基因座的接近性。  |  Kirschner, MA., et al. 1994. Genomics. 24: 218-24. PMID: 7698742
  9. 发育中和成年小鼠中枢神经系统增殖区谷氨酸转运体 mRNA 的表达。  |  Sutherland, ML., et al. 1996. J Neurosci. 16: 2191-207. PMID: 8601800
  10. 人类高亲和力, Na（+）依赖性 L-谷氨酸/L-天门冬氨酸转运体 GLAST-1 (EAAT-1)：基因结构和在染色体 5p11-p12 上的定位。  |  Stoffel, W., et al. 1996. FEBS Lett. 386: 189-93. PMID: 8647279
  11. 核分裂是大脑神经元中谷氨酸兴奋毒性的先导步骤。  |  Ikeda, J., et al. 1996. J Neurosci Res. 43: 613-22. PMID: 8833096
  12. 大鼠视网膜中的高亲和力谷氨酸转运体：神经胶质谷氨酸转运体 GLAST-1 在清除递质中的主要作用。  |  Rauen, T., et al. 1998. Cell Tissue Res. 291: 19-31. PMID: 9394040

订购信息

产品名称产品编号规格价格数量收藏夹

EAAT2 抗体 (E-1)

sc-365634
200 µg/ml
$316.00

EAAT2 (E-1): m-IgG Fc BP-HRP 套装

sc-529417
200 µg Ab; 10 µg BP
$354.00

EAAT2 (E-1): m-IgGκ BP-HRP 套装

sc-522303
200 µg Ab, 40 µg BP
$354.00

EAAT2 (E-1): m-IgG2b BP-HRP 套装

sc-550107
200 µg Ab; 10 µg BP
$354.00

EAAT2 抗体 (E-1) AC

sc-365634 AC
500 µg/ml, 25% agarose
$416.00

EAAT2 抗体 (E-1) HRP

sc-365634 HRP
200 µg/ml
$316.00

EAAT2 抗体 (E-1) FITC

sc-365634 FITC
200 µg/ml
$330.00

EAAT2 抗体 (E-1) PE

sc-365634 PE
200 µg/ml
$343.00

EAAT2 抗体 (E-1) Alexa Fluor® 488

sc-365634 AF488
200 µg/ml
$357.00

EAAT2 抗体 (E-1) Alexa Fluor® 546

sc-365634 AF546
200 µg/ml
$357.00

EAAT2 抗体 (E-1) Alexa Fluor® 594

sc-365634 AF594
200 µg/ml
$357.00

EAAT2 抗体 (E-1) Alexa Fluor® 647

sc-365634 AF647
200 µg/ml
$357.00

EAAT2 抗体 (E-1) Alexa Fluor® 680

sc-365634 AF680
200 µg/ml
$357.00

EAAT2 抗体 (E-1) Alexa Fluor® 790

sc-365634 AF790
200 µg/ml
$357.00

EAAT2 Antibody (E-1) Have you any more information since a year ago as to whether this antibody works in flow cytometry ?

Asked by: DannyK
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-11-20

Is it possible to use this antibody with flow cytometry? is it against to an extracellular epitope?

Asked by: paola64
Thank you for your question. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates! This monoclonal antibody is raised against amino acids 1-85 mapping near the N-terminus of EAAT2, which encompasses potentially both cytoplasmic (aa 1-44) and extracellular (65-87) domains. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-09-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with EAAT2 Antibody (E-1): sc-365634?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 5 out of 5 by from buon funzionamento in wbTestato su una linea di astrocitoma umana in wb. Funziona molto bene
Date published: 2023-03-10
Rated 5 out of 5 by from Antibodies for western blotGLT1 (1:1000, Santa Cruz Biotechnology Cat# sc-365634,RRID:AB_ 10844832), GLAST (1:1000 Santa Cruz Biotechnology Cat# sc-515839) ERK (1:1000 Santa Cruz Biotechnology Cat# sc135900, RRID:AB_2141283) or GAPDH (1:10000 Santa Cruz Biotechnology Cat# sc-25778 RRID: AB_10167668)
Date published: 2020-10-28
Rated 4 out of 5 by from buon risultato sia in immunofluorescenza sia in wbHo utilizzato questo anticorpo sia in immunofluorescenza (diluizione usata 1:100), che in wb (usato 1:1000) su midollo spinale di topo e sono rimasta molto soddisfatta del risultato ottenuto
Date published: 2020-10-06
Rated 5 out of 5 by from Good antibody for WBThe bands were very dark at 1:200 dilution. We used 1:400 dilution and still had clear bands
Date published: 2020-09-01
Rated 4 out of 5 by from よく染まります。サンプルをもらってアストロサイト細胞株(C8-D1A)を染めました。1:100-1:500で非常にきれいに染まります。
Date published: 2020-01-16
Rated 5 out of 5 by from Ottimo funzionamento!Ho provato il campione trial (western blot) ed ho ottenuto una banda pulita come da immagine rappresentativa, sono molto soddisfatta del risultato ottenuto.
Date published: 2019-02-25
Rated 5 out of 5 by from EAAT2 Antibody (E-1)Quick support, short response time, excellent product
Date published: 2018-08-08
Rated 5 out of 5 by from Excellent resultWorks great with brain homogenate, using 1:500 dilution
Date published: 2018-02-14
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EAAT2 抗体 (E-1) is rated 4.9 out of 5 by 21.
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