Date published: 2025-9-8

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Dynein IC1/2, cytosolic 抗体 (74-1): sc-13524

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说明书
  • Dynein IC1/2, cytosolic 抗体 74-1 是小鼠单克隆 IgG2b κ,Dynein IC1/2, cytosolic抗体, 在77篇文献中引用,规格为200 µg/ml
  • 针对bovine来源的纯化脑组织细胞质动力蛋白
  • Dynein IC1/2, cytosolic 抗体 (74-1) 推荐用于WB, IP, IF 和 FCM,检测broad species 来源的 intermediate chains 1 and 2 of cytosolic Dynein
  • 抗Dynein IC1/2, cytosolic抗体(74-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dynein IC1/2, cytosolic (74-1): sc-13524免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是Dynein IC1/2, cytosolic Antibody (74-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dynein IC1/2, cytosolic Antibody (74-1) 打包提供(请参阅下面的订购信息)。

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関連項目

细胞质抗体(74-1)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测多种物种来源的细胞质蛋白Dynein IC1/2。细胞质(74-1)驱动蛋白IC1/2抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。动力蛋白是重要的多亚基ATP酶,通过与微管相互作用,促进细胞货物向细胞中心移动,在细胞运输中发挥着关键作用。Dynein IC1和IC2蛋白是细胞质中动力蛋白复合物的组成部分,该复合物由大约12个亚基组成,包括两个重链和两个中间链,它们将动力蛋白锚定到其货物上。该复合物对于维持细胞完整性和功能至关重要的各种细胞功能(如细胞器运输和中心体组装)至关重要。动力蛋白的羧基末端对于微管依赖性运动尤为重要,并且在物种间高度保守,而氨基末端区域则表现出更大的变异性,从而实现功能多样性。此外,动力蛋白的活性受多种蛋白调节,包括动力蛋白、LIS1和NudEL(NudE样),它们对于动力蛋白马达在细胞过程中的正常运作至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dynein IC1/2, cytosolic 抗体 (74-1) 参考文献:

  1. 细胞质动力蛋白在负载时发挥齿轮的功能。  |  Mallik, R., et al. 2004. Nature. 427: 649-52. PMID: 14961123
  2. 细胞质动力蛋白对微管进行核化,从而在体内将微管组织成径向阵列。  |  Malikov, V., et al. 2004. Mol Biol Cell. 15: 2742-9. PMID: 15047865
  3. 动力蛋白重链家族  |  Asai, DJ. and Wilkes, DE. 2004. J Eukaryot Microbiol. 51: 23-9. PMID: 15068262
  4. 外臂动力蛋白产生的轴突微管高速滑动。  |  Seetharam, RN. and Satir, P. 2005. Cell Motil Cytoskeleton. 60: 96-103. PMID: 15605357
  5. 动力蛋白功能的卸载模型:运动亚基的不同功能。  |  Lee, WL., et al. 2005. J Cell Biol. 168: 201-7. PMID: 15642746
  6. 细胞质动力蛋白在微管和神经丝轴突运输中的作用  |  He, Y., et al. 2005. J Cell Biol. 168: 697-703. PMID: 15728192
  7. NudEL 通过 LIS1 将动力蛋白定向到微管末端。  |  Li, J., et al. 2005. Nat Cell Biol. 7: 686-90. PMID: 15965467
  8. 细胞质动力蛋白命名法。  |  Pfister, KK., et al. 2005. J Cell Biol. 171: 411-3. PMID: 16260502
  9. Dynein 运动:四个头比两个头好。  |  McGrath, JL. 2005. Curr Biol. 15: R970-2. PMID: 16332531
  10. 细胞质动力蛋白亚基家族的遗传分析。  |  Pfister, KK., et al. 2006. PLoS Genet. 2: e1. PMID: 16440056

订购信息

产品名称产品编号规格价格数量收藏夹

Dynein IC1/2, cytosolic 抗体 (74-1)

sc-13524
200 µg/ml
$316.00

Dynein IC1/2, cytosolic (74-1): m-IgG Fc BP-HRP 套装

sc-528285
200 µg Ab; 10 µg BP
$354.00

Dynein IC1/2, cytosolic (74-1): m-IgGκ BP-HRP 套装

sc-520624
200 µg Ab, 40 µg BP
$354.00

Dynein IC1/2, cytosolic (74-1): m-IgG2b BP-HRP 套装

sc-550023
200 µg Ab; 10 µg BP
$354.00

Dynein IC1/2, cytosolic 抗体 (74-1) AC

sc-13524 AC
500 µg/ml, 25% agarose
$416.00

Dynein IC1/2, cytosolic 抗体 (74-1) HRP

sc-13524 HRP
200 µg/ml
$316.00

Dynein IC1/2, cytosolic 抗体 (74-1) FITC

sc-13524 FITC
200 µg/ml
$330.00

Dynein IC1/2, cytosolic 抗体 (74-1) PE

sc-13524 PE
200 µg/ml
$343.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 488

sc-13524 AF488
200 µg/ml
$357.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 546

sc-13524 AF546
200 µg/ml
$357.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 594

sc-13524 AF594
200 µg/ml
$357.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 647

sc-13524 AF647
200 µg/ml
$357.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 680

sc-13524 AF680
200 µg/ml
$357.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 790

sc-13524 AF790
200 µg/ml
$357.00

Would the antibody sc-13524 detect porcine dynein?

Asked by: DinaA
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-09

What is the recommended secondary for this mouse monoclonal primary?

Asked by: AbPolly
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-20

Can this antibody be used with Xenopus samples?

Asked by: AbPolly
This antibody is recommended for use with a broad range of species. For specific species, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data.
Answered by: Technical Support 14
Date published: 2019-03-04
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Rated 5 out of 5 by from Great antibodyIt seems the best commercial DIC antibody which can be used for both WB and IP. Excellent.
Date published: 2020-11-17
Rated 5 out of 5 by from Good for IFThis Dynein IC1/2, cytosolic Antibody (74-1), sc-13524, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from Works well for Western BlotWe used this antibody in CV-1(monkey) cell line after performing a western blot. 1:2000 in milk. The bands were clear with very little background.
Date published: 2017-11-01
Rated 5 out of 5 by from Great productOutstanding product for both western blot and IF staining applications
Date published: 2017-01-20
Rated 5 out of 5 by from Strong signalThis Dynein IC1/2, cytosolic antibody shows strong signal in WB.
Date published: 2017-01-12
Rated 5 out of 5 by from Great in WB!!!Detects a single band in HeLa lysate at a 1:500 dilution. VERY strong single.
Date published: 2016-11-29
Rated 5 out of 5 by from Positive western blot detection of Dynein IC1/2Positive western blot detection of Dynein IC1/2, cytosolic expression in T98G, IMR-32 and L8 whole cell lysates. -SCBT QC
Date published: 2015-07-23
Rated 5 out of 5 by from Great intracellular FCM data of fixed andGreat intracellular FCM data of fixed and permeabilized L8 cells. -SCBT QC
Date published: 2015-06-01
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Dynein IC1/2, cytosolic 抗体 (74-1) is rated 5.0 out of 5 by 11.
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