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Dok-2 抗体 (E-10): sc-17830

4.5(6)
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说明书
  • Dok-2 抗体 E-10 是小鼠单克隆 IgG1 κ,Dok-2抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫human来源Dok-2位于C-terminus 的221-412氨基酸
  • Dok-2 抗体 (E-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Dok-2
  • 抗Dok-2抗体(E-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dok-2 (E-10): sc-17830免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Dok-2 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dok-2 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Dok-2抗体(E-10)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定( 酶联免疫吸附试验)。抗Dok-2抗体(E-10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Dok-2,又称p56 Dok,在细胞信号传导通路中起着至关重要的作用,特别是在生长因子受体激活方面。Dok-2主要位于细胞质中,负责调节Ras信号传导,而Ras信号传导对于细胞增殖和分化至关重要。Dok-2与Ras GTP酶激活蛋白相互作用,凸显了Dok-2在调节Ras活性方面的重要性,而Ras活性是控制细胞生长和存活的许多信号级联中的关键因素。该信号通路失调可导致包括癌症在内的多种疾病,因此Dok-2及其相互作用对于理解肿瘤发生和开发靶向疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dok-2 抗体 (E-10) 参考文献:

  1. 有证据表明,SH2 结构域可促进蛋白酪氨酸激酶的磷酸化过程。  |  Mayer, BJ., et al. 1995. Curr Biol. 5: 296-305. PMID: 7780740
  2. 血管内皮细胞生长因子促进含有 SH2 结构域的信号转导介质的酪氨酸磷酸化。与内皮细胞增殖有关。  |  Guo, D., et al. 1995. J Biol Chem. 270: 6729-33. PMID: 7896817
  3. IRS-1 信号系统  |  Myers, MG., et al. 1994. Trends Biochem Sci. 19: 289-93. PMID: 8048169
  4. 一种 62 千道尔顿的酪氨酸磷酸蛋白,在原发性慢性期慢性髓性白血病富集的阴性血系爆炸群中连续存在。  |  Wisniewski, D., et al. 1994. Leukemia. 8: 688-93. PMID: 8152267
  5. 一种在表皮生长因子和胰岛素受体信号传导过程中与 Grb2 相关的对接蛋白。  |  Holgado-Madruga, M., et al. 1996. Nature. 379: 560-4. PMID: 8596638
  6. p62(dok):慢性骨髓性白血病祖细胞中一种组成型酪氨酸磷酸化的 GAP 缔合作用蛋白。  |  Carpino, N., et al. 1997. Cell. 88: 197-204. PMID: 9008160
  7. 鉴定与 Abl 和 rasGAP 相关的 62 kDa 蛋白为对接蛋白,Dok.  |  Yamanashi, Y. and Baltimore, D. 1997. Cell. 88: 205-11. PMID: 9008161
  8. p56dok-2 的分子克隆和特征描述定义了一个新的 RasGAP 结合蛋白家族。  |  Di Cristofano, A., et al. 1998. J Biol Chem. 273: 4827-30. PMID: 9478921

订购信息

产品名称产品编号规格价格数量收藏夹

Dok-2 抗体 (E-10)

sc-17830
200 µg/ml
$316.00

Dok-2 (E-10): m-IgG Fc BP-HRP 套装

sc-528329
200 µg Ab; 10 µg BP
$354.00

Dok-2 (E-10): m-IgGκ BP-HRP 套装

sc-520675
200 µg Ab, 40 µg BP
$354.00

Dok-2 (E-10): m-IgG1 BP-HRP 套装

sc-542869
200 µg Ab; 20 µg BP
$354.00

Dok-2 抗体 (E-10) AC

sc-17830 AC
500 µg/ml, 25% agarose
$416.00

Dok-2 抗体 (E-10) HRP

sc-17830 HRP
200 µg/ml
$316.00

Dok-2 抗体 (E-10) FITC

sc-17830 FITC
200 µg/ml
$330.00

Dok-2 抗体 (E-10) PE

sc-17830 PE
200 µg/ml
$343.00

Dok-2 抗体 (E-10) Alexa Fluor® 488

sc-17830 AF488
200 µg/ml
$357.00

Dok-2 抗体 (E-10) Alexa Fluor® 546

sc-17830 AF546
200 µg/ml
$357.00

Dok-2 抗体 (E-10) Alexa Fluor® 594

sc-17830 AF594
200 µg/ml
$357.00

Dok-2 抗体 (E-10) Alexa Fluor® 647

sc-17830 AF647
200 µg/ml
$357.00

Dok-2 抗体 (E-10) Alexa Fluor® 680

sc-17830 AF680
200 µg/ml
$357.00

Dok-2 抗体 (E-10) Alexa Fluor® 790

sc-17830 AF790
200 µg/ml
$357.00

I am using Dok-2 (E-10): sc-17830 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human colorectal cancer -SCBT Publication Review
Date published: 2015-06-18
Rated 4 out of 5 by from Very good size band used on human DokVery good size band used on human Dok-2 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by from Very good WB results using human DokVery good WB results using human Dok-2 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic stainingSuperb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-03-21
Rated 4 out of 5 by from Strong WB signal obtained using MEGStrong WB signal obtained using MEG-01 whole cell lysate. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Published data for IP and WB in HutPublished data for IP and WB in Hut-78 cells -SCBT Publication Review
Date published: 2015-01-23
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Dok-2 抗体 (E-10) is rated 4.5 out of 5 by 6.
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