Dimethyl Sulfoxide (DMSO), cell culture reagent CAS: 67-68-5
MF: C2H6OS
MW: 78.13
A polar, aprotic organic solvent ideal for cryopreservation.

Dimethyl Sulfoxide (DMSO), cell culture reagent (CAS 67-68-5)

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同义词: methylsulfinylmethane
应用; A polar, aprotic organic solvent ideal for cryopreservation
CAS号码: 67-68-5
纯度: ≥99%
分子量: 78.13
分子式: C2H6OS
* 参考分析证明 大量特定数据 (包括水 含量).
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Dimethyl Sulfoxide (DMSO), cell culture reagent is ideal for cryopreservation. DMSO is a polar, aprotic organic solvent with many applications in chemical and biological research. DMSO can uniquely provide solubility to certain very polar small molecules, such as simple biomolecules with multiple protic functional groups. It can also be used as an oxidation reagent in certain transformations. The yield of PCR processes is amplified with DMSO as a co-solvent, helping to inhibit the formation of secondary structures from DNA fragments.


参考文献

1. Sweat, F.W. and Epstein, W.W. 1967. J. Org. Chem. 32: 835-838. PMID: 6042131
2. Winship, P.R. 1989. Nucleic Acids Res. 17: 1266. PMID: 2922271
3. Chakrabarti, R. and Schutt, C.E. 2001. Gene. 274: 293-298. PMID: 11675022
4. Heidemann, R., et al. 2010. Biotechnol. Prog. 26: 1154-1163. PMID: 20730770
5. Thirumala, S., et al. 2010. Stem Cells Dev. 19: 513-522. PMID: 19788372

用法 :
Dimethyl Sulfoxide (DMSO), Cell Culture reagent is used in molecular biology, especially in the polymerase chain reaction (PCR), cell lysis, in transformation and transfection and in cytofluorimetric assessment. DMSO is most often used at a concentration of 10%.
物理状态 :
Liquid
溶解度 :
Soluble in water, methanol, acetone, ether, chloroform, benzene, and ethanol. DMSO is miscible in most of the common organic solvents such as alcohols, esters, ketones, ethers, chlorinated solvents and aromatics.
保存 :
Desiccate at room temperature
熔点 :
16-19° C (lit.)
沸点 :
189° C at 101.3 kPa
密度 :
1.10 g/mL(lit.)
折射率 :
n20D 1.48 (lit.)
IC50 :
HII4E cells : IC50 = 199 µg.mL-1 (mouse)
Ki 数据 :
Choline acetylase: Ki= 25 mM (rat)
pK值 :
pKa: -4.54 (Predicted)
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
1
RTECS :
PV6210000
PubChem CID :
679
默克索引 :
14: 3259
MDL 号码 :
MFCD00002089
EC号码 :
200-664-3
Beilstein 注册 :
506008
SMILES :
CS(=O)C

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PMID: # 31068616  Myszor, IT.|Parveen, Z.|Ottosson, H.|Bergman, P.|Agerberth, B.|Strömberg, R.|Gudmundsson, GH.| et al. 2019. Sci Rep. 9: 7114.

PMID: # 31031137  Kalkan, T.|Bornelöv, S.|Mulas, C.|Diamanti, E.|Lohoff, T.|Ralser, M.|Middelkamp, S.|Lombard, P.|Nichols, J.|Smith, A.| et al. 2019. Cell Stem Cell. 24: 785-801.e7.

PMID: # 31249607  Li, J.|Dang, N.|Martinez-Lopez, N.|Jowsey, PA.|Huang, D.|Lightowlers, RN.|Gao, F.|Huang, JY.| et al. 2019. Cell Div. 14: 5.

PMID: # 29556174  Li, X.|Peng, J.|Pang, J.|Wu, Y.|Huang, X.|Li, Y.|Zhou, J.|Gu, L.|Sun, X.|Chen, L.|Vitek, MP.|Jiang, Y.| et al. 2018. Front Neurosci. 12: 127.

PMID: # 29805036  2018. Cell Chem Biol. 25: 749-760.e9.

PMID: # 27709569  2017. Methods Mol. Biol. 1498: 57-78.

PMID: # 29163636  House, JS. et al. 2017. Front Genet. 8: 168.

PMID: # 27179306  Li, X. et al. 2016. Int. Immunopharmacol. 36: 263-70.

PMID: # 26895409  Mizokami, SS. et al. 2016. PLoS ONE. 11: e0149656.

PMID: # 26386269  Kang, W. et al. 2016. Journal of biomedical materials research. Part A. 104: 283-90.

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