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demethylated-PP2A-C Antibody (4B7) - Western Blotting - Image 359157
去甲基化-PP2A-C 抗体 (4B7): sc-13601. MCF7 (A), A-431 (B), U-937 (C), HL-60 (D), MOLT-4 (E) 和 KNRK (F) 全细胞裂解液中去甲基化-PP2A-C 表达的 Western 印迹分析.
去甲基化-PP2A-C 抗体 (4B7): sc-13601. MCF7 (A), A-431 (B), U-937 (C), HL-60 (D), MOLT-4 (E) 和 KNRK (F) 全细胞裂解液中去甲基化-PP2A-C 表达的 Western 印迹分析.

demethylated-PP2A-C 抗体 (4B7): sc-13601

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寫評論提問

说明书
  • demethylated-PP2A-C 抗体 4B7 是小鼠单克隆 IgG1 κ，demethylated-PP2A-C抗体, 在36篇文献中引用，规格为100 µg/ml
  • 针对PP2A-C亚基的未甲基化的C-terminal区域
  • demethylated-PP2A-C 抗体 (4B7) 推荐用于WB, IP, IF 和 IHC(P)， 检测 mouse, rat, human, Drosophila, Xenopus 和 S. pombe 来源的 demethylated-PP2A-C; 进行去甲基化，在冰上用100mM NaOH处理
  • 抗demethylated-PP2A-C抗体（4B7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取demethylated-PP2A-C (4B7): sc-13601的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 demethylated-PP2A-C 抗体 (4B7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与demethylated-PP2A-C 抗体 (4B7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

此外 PP2 抗体 包括 PP2A-A α, PP2A-A alpha / β, PP2A-B55, PP2A-B55-α, PP2A-B55-γ, PP2A-B56-α, PP2A-B56-δ, PP2A-B56-γ, PP2A-C和PP2A-C alpha / β

去甲基化-PP2A-C抗体（4B7）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组化与 石蜡包埋切片（IHCP）检测。去甲基化-PP2A-C（4B7）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（ ）和多种Alexa Fluor®偶联物。去甲基化-PP2A-C蛋白在细胞信号传导通路中发挥着关键作用，特别是在细胞生长和分裂的调节中，因为去甲基化-PP2A-C是蛋白磷酸酶2A（PP2A）复合物的重要组成部分。该复合物对于去磷酸化目标蛋白上的丝氨酸和苏氨酸残基至关重要，从而调节其活性和功能。PP2A可维持细胞内环境稳定，防止细胞无序增殖，而细胞无序增殖正是癌症的典型特征。此外，PP2A催化亚基与各种调节亚基（如PP2A-Aα、PP2A-Bα等）相互作用，这些调节亚基决定了磷酸酶的底物特异性和定位，进一步凸显了PP2A在细胞过程中的复杂性和重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

demethylated-PP2A-C 抗体 (4B7) 参考文献:

  1. PP2A催化亚基的甲基化C末端亮氨酸残基对于调节亚基Balpha的结合非常重要。  |  Bryant, JC., et al. 1999. Biochem J. 339 (Pt 2): 241-6. PMID: 10191253
  2. PP2A催化亚基在Wnt信号转导中发挥积极作用。  |  Ratcliffe, MJ., et al. 2000. J Biol Chem. 275: 35680-3. PMID: 11007767
  3. PP2A催化亚基的C末端亮氨酸残基的甲基化对于催化活性和调节亚基（PR55/B）的结合是不必要的。  |  Ikehara, T., et al. 2007. Biochem Biophys Res Commun. 354: 1052-7. PMID: 17274953
  4. (DEAD)-box RNA 螺旋酶 3 通过控制 PP2A-C 亚基的磷酸化来调节 NF-κB 信号通路。  |  Wang, X., et al. 2017. Oncotarget. 8: 33197-33213. PMID: 28402257
  5. 通过microRNA-429沉默PP2A催化亚基激活AMPK并保护成骨细胞免受地塞米松的影响。  |  Guo, S., et al. 2017. Biochem Biophys Res Commun. 487: 660-665. PMID: 28438603
  6. 伊伐布雷定激活PP2A催化亚基可减轻糖尿病心肌病的发展。  |  Zuo, GF., et al. 2019. J Mol Cell Cardiol. 130: 170-183. PMID: 30998977
  7. 识别 PP2A 催化亚基 C 末端的抗体不适合用于评估 PP2A 活性和全酶组成。  |  Frohner, IE., et al. 2020. Sci Signal. 13: PMID: 31992581
  8. PP2A-C可能是估计死后间隔的有前途的候选者。  |  Wang, J., et al. 2021. Int J Legal Med. 135: 837-844. PMID: 33409557
  9. PP2A催化亚基α通过ERK途径促进成纤维细胞活化和肾脏纤维化。  |  Lu, Q., et al. 2022. Cell Signal. 90: 110187. PMID: 34780974
  10. PP2A催化亚基α对于CD8+ T细胞稳态和抗菌反应至关重要。  |  Zhou, X., et al. 2024. Eur J Immunol. 54: e2451080. PMID: 39072720

订购信息

产品名称产品编号规格价格数量收藏夹

demethylated-PP2A-C 抗体 (4B7)

sc-13601
100 µg/ml
$316.00

demethylated-PP2A-C (4B7): m-IgG Fc BP-HRP 套装

sc-528292
100 µg Ab; 10 µg BP
$354.00

demethylated-PP2A-C (4B7): m-IgGκ BP-HRP 套装

sc-520633
100 µg Ab, 40 µg BP
$354.00

demethylated-PP2A-C (4B7): m-IgG1 BP-HRP 套装

sc-542854
100 µg Ab; 20 µg BP
$354.00

demethylated-PP2A-C 抗体 (4B7) AC

sc-13601 AC
500 µg/ml, 25% agarose
$416.00

demethylated-PP2A-C 抗体 (4B7) HRP

sc-13601 HRP
200 µg/ml
$316.00

demethylated-PP2A-C 抗体 (4B7) FITC

sc-13601 FITC
200 µg/ml
$330.00

demethylated-PP2A-C 抗体 (4B7) PE

sc-13601 PE
200 µg/ml
$343.00

demethylated-PP2A-C 抗体 (4B7) Alexa Fluor® 488

sc-13601 AF488
200 µg/ml
$357.00

demethylated-PP2A-C 抗体 (4B7) Alexa Fluor® 546

sc-13601 AF546
200 µg/ml
$357.00

demethylated-PP2A-C 抗体 (4B7) Alexa Fluor® 594

sc-13601 AF594
200 µg/ml
$357.00

demethylated-PP2A-C 抗体 (4B7) Alexa Fluor® 647

sc-13601 AF647
200 µg/ml
$357.00

demethylated-PP2A-C 抗体 (4B7) Alexa Fluor® 680

sc-13601 AF680
200 µg/ml
$357.00

demethylated-PP2A-C 抗体 (4B7) Alexa Fluor® 790

sc-13601 AF790
200 µg/ml
$357.00

can I use it for ICC? PFA 4% fixed

Asked by: sherry z
Thank you for your question! Yes, this antibody is fine for use with immunocytochemistry. You can find our recommended protocols here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-03-08

Can demethylated-PP2A-C (4B7): sc-13601 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, demethylated-PP2A-C (4B7): sc-13601 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from WBで使用しました。WBにて使用した結果です. 条件は写真に記しています。 HEK293T細胞10cm 1枚分のタンパク量です。刺激をしていないので 発現が見られないのかもしれませんが、バンドはあまりよくないと思います。
Date published: 2020-11-04
Rated 5 out of 5 by from demethylated PP2A antibodyDemethylated-PP2A-C (4B7) (sc-13601) works excellently in the lab.
Date published: 2018-09-24
Rated 1 out of 5 by from Pp2a- c antibodyDid not work for fission yeast as was written in the advert
Date published: 2018-03-07
Rated 5 out of 5 by from good antibody for WBIt works well in WB at 1:1000 in mice brain tissues. Strip is very clear, the location is very accurate
Date published: 2017-10-08
Rated 5 out of 5 by from Great featuresI have been using this antibody since one month ago. It works well in WB at 1:1000 in mice brain tissues. thank you.
Date published: 2017-09-29
Rated 4 out of 5 by from Good antibody for western blottinThis antibody could use for western blotting in SK-EHP-1 cells. It could detect two bands of PP2A. I'm not sure whether the antibody detected is demethylated-PP2A-C or just PP2A-C.
Date published: 2017-09-16
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of demethylated-PP2A-C expression in MCF7, NIH/3T3 and KNRK whole cell lysates.
Date published: 2017-01-30
Rated 5 out of 5 by from ExcellentExcellent!
Date published: 2015-12-10
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demethylated-PP2A-C 抗体 (4B7) is rated 4.1 out of 5 by 14.
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