Date published: 2025-9-5

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DEDD 抗体 (H-4): sc-271192

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说明书
  • DEDD 抗体 H-4 是小鼠单克隆 IgG1 κ,DEDD抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的DEDD的C-terminus附近的氨基酸278-313之间
  • DEDD 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 DEDD; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗DEDD抗体(H-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DEDD (H-4): sc-271192免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 DEDD 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与DEDD 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

DEDD抗体(H-4)是一种IgG1 κ小鼠单克隆DEDD抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的DEDD蛋白。DEDD抗体(H-4)既可作为非共轭的抗DEDD抗体形式,也可作为多种共轭的抗DEDD抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。凋亡是多细胞生物体消除不需要的细胞的生理过程。DEDD(含死亡效应结构域的DNA结合蛋白)通过触发由N端DED基序介导的一系列细胞内蛋白质-蛋白质相互作用来诱导凋亡。DEDD是一种细胞质蛋白,在CD95介导的凋亡过程中转位到细胞核,在那里它定位于核仁样结构,激活caspase-6并特异性抑制RNA聚合酶I依赖性转录。DEDD的细胞死亡活性与其核定位有关。DEDD中的DED足以使其结合DNA、激活capspase-6并抑制Pol I的特异性转录。点特异性突变表明,DEDD中的DED代表了一个新型结构域,其结构与其他DED相似,但功能与FADD或caspase-8中的经典DED不同。DEDD在多种组织中广泛表达,在睾丸中表达水平最高。人类DEDD基因定位于染色体1q23.3。选择性剪接产生两种转录变体,编码相同的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DEDD 抗体 (H-4) 参考文献:

  1. DEDD 的核定位可通过其死亡效应结构域激活 Caspase-6,并抑制 RNA 聚合酶 I 依赖性转录。  |  Schickling, O., et al. 2001. Cell Death Differ. 8: 1157-68. PMID: 11753564
  2. DEDD 和 DEDD2 与 Caspase-8/10 结合,发出细胞死亡信号。  |  Alcivar, A., et al. 2003. Oncogene. 22: 291-7. PMID: 12527898
  3. DEDD是一种靶向于核仁的新型含死亡效应域蛋白。  |  Stegh, AH., et al. 1998. EMBO J. 17: 5974-86. PMID: 9774341
  4. DEFT,一种主要在睾丸生殖细胞中表达的新型含死亡效应域分子。  |  Leo, CP., et al. 1998. Endocrinology. 139: 4839-48. PMID: 9832420

订购信息

产品名称产品编号规格价格数量收藏夹

DEDD 抗体 (H-4)

sc-271192
200 µg/ml
$316.00

DEDD (H-4): m-IgG Fc BP-HRP 套装

sc-527218
200 µg Ab; 10 µg BP
$354.00

DEDD (H-4): m-IgG1 BP-HRP 套装

sc-532591
200 µg Ab; 20 µg BP
$354.00

DEDD 抗体 (H-4) AC

sc-271192 AC
500 µg/ml, 25% agarose
$416.00

DEDD 抗体 (H-4) HRP

sc-271192 HRP
200 µg/ml
$316.00

DEDD 抗体 (H-4) FITC

sc-271192 FITC
200 µg/ml
$330.00

DEDD 抗体 (H-4) PE

sc-271192 PE
200 µg/ml
$343.00

DEDD 抗体 (H-4) Alexa Fluor® 488

sc-271192 AF488
200 µg/ml
$357.00

DEDD 抗体 (H-4) Alexa Fluor® 546

sc-271192 AF546
200 µg/ml
$357.00

DEDD 抗体 (H-4) Alexa Fluor® 594

sc-271192 AF594
200 µg/ml
$357.00

DEDD 抗体 (H-4) Alexa Fluor® 647

sc-271192 AF647
200 µg/ml
$357.00

DEDD 抗体 (H-4) Alexa Fluor® 680

sc-271192 AF680
200 µg/ml
$357.00

DEDD 抗体 (H-4) Alexa Fluor® 790

sc-271192 AF790
200 µg/ml
$357.00

DEDD (H-4) 中和勝肽

sc-271192 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271192 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Works nicely in WB and IP and IHC.I have been looking for anti-DEDD antibody for couple years. The protein is not well studied and there were not many companies developing this antibody. The previous studies focused on this protein had been cloning tags attached DEDD and used other tag-detected antibodies. I tried only two DEDD antibodies from other two companies and they never worked. This year earlier I surprisingly found that Santa Cruz has anti-DEDD antibody available so I just bought it. It wokrs very well in WB, as well as in IF, IP and IHC. And non-specific bands were very weak compared to the DEDD band.
Date published: 2016-12-06
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-03-09
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DEDD 抗体 (H-4) is rated 5.0 out of 5 by 2.
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