DBR1 CRISPR/Cas9 KO Plasmid (h): sc-407375
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DBR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DBR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DBR1 Antibody (3A7): sc-517060
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DBR1 CRISPR/Cas9 KO Plasmid (h) | sc-407375 | 20 µg | $397.00 |
Overview
DBR1 encodes RNA lariat debranching enzyme 1, a conserved phosphodiesterase that hydrolyzes the 2′–5′ phosphodiester bond at intron lariat branch points to linearize excised introns for turnover. This activity links DBR1 to spliceosome-dependent RNA processing, intron decay pathways, and maintenance of RNA homeostasis that can influence gene expression programs. Perturbation of lariat debranching can alter the balance of circular and linear RNA species and reshape post-transcriptional regulation under stress and differentiation contexts. Accordingly, DBR1 dysfunction has been associated with neurological and viral susceptibility phenotypes in the literature, supporting its relevance for mechanistic studies of RNA metabolism in disease-relevant cell types.
DBR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DBR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DBR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DBR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DBR1 protein expression.
This CRISPR knockout system enables efficient generation of DBR1-deficient cell models for investigation of DBR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DBR1 exon(s) critical for DBR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DBR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DBR1 CRISPR/Cas9 KO Plasmid (h) and DBR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DBR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DBR1 HDR Plasmid (h) and DBR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DBR1 homology arms to support homology-directed repair at defined DBR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.