Date published: 2026-10-16
访问圣克鲁斯动物健康

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6) - Western Blotting - Image 109575
DAT 抗体 (6-8D6): sc-32259. SH-SY5Y (A) 和 SK-N-SH (B) 全细胞裂解液中 DAT 表达的 Western 印迹分析.
DAT 抗体 (6-8D6): sc-32259. SH-SY5Y (A) 和 SK-N-SH (B) 全细胞裂解液中 DAT 表达的 Western 印迹分析.

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6): sc-32259

4.5(11)
寫評論提問
说明书
  • DAT/Dopamine Transporter/SLC6A3 抗体 6-8D6 是大鼠单克隆 IgG2a，DAT/Dopamine Transporter/SLC6A3抗体, 在20篇文献中引用，规格为200 µg/ml
  • 针对氨基酸180-218的抗性，对应DAT的第二个细胞外环，human起源
  • DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 DAT
  • 抗DAT/Dopamine Transporter/SLC6A3抗体（6-8D6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 目前，我们还没有完成DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6)的首选二抗检测试剂的鉴定。这项工作正在进行中。
Gene Editing Promo Banner

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

DAT抗体（6-8D6）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的DAT蛋白。抗DAT抗体（6-8D6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。多巴胺转运蛋白（DAT）通过促进多巴胺从细胞外空间重新摄取回突触前神经元，在调节突触间隙的多巴胺水平方面发挥着至关重要的作用。这一功能对于维持多巴胺的稳态至关重要，而多巴胺稳态对于正常的神经功能和行为必不可少。DAT活性的失调与多种神经精神疾病有关，包括注意力缺陷多动障碍（ADHD）和药物滥用障碍，这凸显了DAT在健康和疾病中的重要性。DAT与多种神经毒素的相互作用凸显了其在神经退行性疾病中的重要性，尤其是在帕金森病中，DAT功能障碍会导致多巴胺能神经元的选择性脆弱性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) 参考文献:

  1. 多巴胺转运体（DAT）在多巴胺跨鼻黏膜转运中的作用。  |  Chemuturi, NV., et al. 2006. Life Sci. 79: 1391-8. PMID: 16733058
  2. 多巴胺转运蛋白（DAT）抑制剂可缓解猴子的特定帕金森氏症缺陷：与体内DAT占用有关。  |  Madras, BK., et al. 2006. J Pharmacol Exp Ther. 319: 570-85. PMID: 16885433
  3. 抗精神病药引起的锥体外系症状的多巴胺转运蛋白（DAT）基因型（VNTR）和表型。  |  Lafuente, A., et al. 2007. Schizophr Res. 90: 115-22. PMID: 17150335
  4. 多巴胺转运蛋白（DAT）在种马精子中的存在和功能：多巴胺调节精子活力和顶体完整性。  |  Urra, JA., et al. 2014. PLoS One. 9: e112834. PMID: 25402186
  5. 小鼠多巴胺转运蛋白（DAT）基因功能低下可产生与多动症一致的改变，但不会导致精神分裂症或双相情感障碍。  |  Mereu, M., et al. 2017. Neuropharmacology. 121: 179-194. PMID: 28454982
  6. 多巴胺转运蛋白（DAT）功能表征的体外检测。  |  Aggarwal, S. and Mortensen, OV. 2017. Curr Protoc Pharmacol. 79: 12.17.1-12.17.21. PMID: 29261226
  7. 在伏隔核中敲除多巴胺转运蛋白（DAT）可改善成年小鼠的焦虑和抑郁相关行为。  |  Bahi, A. and Dreyer, JL. 2019. Behav Brain Res. 359: 104-115. PMID: 30367968
  8. 多巴胺转运体（DAT）敲除雄性大鼠性行为的改变：行为, 神经化学和脑内微透析研究。  |  Sanna, F., et al. 2020. Front Behav Neurosci. 14: 58. PMID: 32372926
  9. 变构调节剂KM822通过与多巴胺转运蛋白DAT-1相互作用，减弱了安非他明对秀丽隐杆线虫的行为影响。  |  Refai, O., et al. 2022. Mol Pharmacol. 101: 123-131. PMID: 34906999
  10. 早期帕金森病管理中的缺失见解和多巴胺转运体（DAT）扫描的价值。  |  Hafeez, M., et al. 2024. Geriatrics (Basel). 9: PMID: 39451858

订购信息

产品名称产品编号规格价格数量收藏夹

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6)

sc-32259
200 µg/ml
$322.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) AC

sc-32259 AC
500 µg/ml, 25% agarose
$424.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) HRP

sc-32259 HRP
200 µg/ml
$322.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) FITC

sc-32259 FITC
200 µg/ml
$336.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) PE

sc-32259 PE
200 µg/ml
$349.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 488

sc-32259 AF488
200 µg/ml
$364.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 546

sc-32259 AF546
200 µg/ml
$364.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 594

sc-32259 AF594
200 µg/ml
$364.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 647

sc-32259 AF647
200 µg/ml
$364.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 680

sc-32259 AF680
200 µg/ml
$364.00

DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) Alexa Fluor® 790

sc-32259 AF790
200 µg/ml
$364.00

Can SC-32259 be used for live-cell staining?

Asked by: ZHANG
Thank you for your question. SC-32259 could not be used for live cell staining.
Answered by: Zhanglong
Date published: 2026-06-15

Are there reports using this DAT ab for Immunoprecipitation, if so which ones? Could it be used for magnetic isolation by coupling to Dynabeads?

Asked by: andresj1
Thank you for your question. DAT Antibody (6-8D6): sc-32259 has been tested and is recommended for IP applications with DAT of mouse, rat and human origin. As of now there are no publications in which this antibody has been used for immunoprecipitation.
Answered by: Tech Support Europe
Date published: 2020-07-14

Can I use it on rat sections, blocking with normal donkey serum?

Asked by: ann1
Thank you for your question. Yes, this antibody is mouse, rat and human reactive, so using it on rat samples is fine. If you are doing a Western blot we would recommend blocking in 5% milk with TBS-T. You can block in normal donkey serum if you are doing an IHC or IF, especially if you are using a donkey anti-rat secondary antibody. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-06

Is a blocking peptide for with this monoclonal antibody available?

Asked by: AbPolly
Thank you for your question. This DAT antibody sc-32259 is raised against a recombinant protein and not a short synthetic peptide and a blocking peptide is not possible to design for it.
Answered by: Technical Support 7
Date published: 2017-05-16
  • y_2026, m_10, d_14, h_11CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_32259, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by db63 from Works well for mouse brain tissuePurchased for IHC of mouse brain slices fixed in 0.4% PFA. Used 1:50 down to 1:4000 dilutions (in 4% BSA with 0.3% Triton-X100) and saw great staining of straitum.
Date published: 2021-07-15
Rated 4 out of 5 by MarieLonz from great antibodyI acquired the antibody a while ago. Works greatly and as good as other DATmAb from other vendors. We chose to purchase after sampling the product.
Date published: 2019-03-22
Rated 3 out of 5 by Santa Li from Good!I applied this antibody last year. This antibody couldn't use with rats. It's very sad.
Date published: 2018-03-02
Rated 5 out of 5 by a217 from 功能很出色上半年申请的该抗体，用于westernblot实验，样品为N2A细胞，上样量为50 ug，曝光结果较好。
Date published: 2017-09-27
Rated 5 out of 5 by XueChen from ClearnessI used the antibody in WB at 1:300, PC12 cells. The protein band was clear, and there was no other bands. The pictures shows that the protein band is a little narrow, possibly because I used the 10% SDS separation gel.
Date published: 2017-09-18
Rated 5 out of 5 by Purplegrape from thisAdrenergic binding of the beta - adrenergic receptor activates adenylate cyclase, whereas adrenergic binding of the α2 - adrenergic receptor inhibits adenylate cyclase. The action of the molecule can be excitatory or inhibitory, depending on the type of receptor to which it is bound.
Date published: 2017-02-08
Rated 5 out of 5 by Dr Ninau Qelp from DAT (6-8D6) AntibodyWorks great for Western blot (WB), band at 80 kDa. We have not characterized the band above 90 kDa.
Date published: 2017-01-05
Rated 4 out of 5 by nickbibs from Pretty good...Used in IPs and WB and it worked as expected. Stable for years when stored correctly.
Date published: 2016-12-05
  • y_2026, m_10, d_15, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_32259, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
DAT/Dopamine Transporter/SLC6A3 抗体 (6-8D6) is rated 4.5 out of 5 by 11.
  • y_2026, m_10, d_15, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_32259, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 95ms
  • REVIEWS, PRODUCT
1-800-457-3801
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。