Date published: 2025-9-6
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DARPP-32 Antibody (H-3) - Western Blotting - Image 361582
DARPP-32 抗体 (H-3): sc-271111. 在 SH-SY5Y (A), PC-3 (B), SK-N-MC (C), EOC 20 (D) 和 Neuro-2A (E) 全细胞裂解液中 DARPP-32 表达的 Western 印迹分析.
DARPP-32 抗体 (H-3): sc-271111. 在 SH-SY5Y (A), PC-3 (B), SK-N-MC (C), EOC 20 (D) 和 Neuro-2A (E) 全细胞裂解液中 DARPP-32 表达的 Western 印迹分析.

DARPP-32 抗体 (H-3): sc-271111

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说明书
  • DARPP-32 抗体 H-3 是小鼠单克隆 IgG1 κ，DARPP-32抗体, 在34篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的DARPP-32的N-terminus的氨基酸2-34之间
  • DARPP-32 抗体 (H-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的DARPP-32; 也和以下物种反应，包括： 和 canine, bovine and avian
  • 抗DARPP-32抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DARPP-32 (H-3): sc-271111的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 DARPP-32 抗体 (H-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与DARPP-32 抗体 (H-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 DARPP 抗体包括 DARPP-32

DARPP-32抗体（H-3）是一种IgG1 κ小鼠单克隆DARPP-32抗体（也称为PPP1R1B抗体，或蛋白磷酸酶1调节抑制亚基1B抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（P）和酶联免疫吸附试验检测小鼠、大鼠和人源的DARPP-32蛋白。DARPP-32抗体（H-3）既可作为非偶联抗DARPP-32抗体形式存在，也可作为多种偶联形式的抗DARPP-32抗体存在，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。多巴胺能信号通路对大脑的多项功能至关重要，在精神分裂症、帕金森病和药物滥用等多种神经系统疾病中多巴胺能信号通路均呈异常。DARPP-32（用于多巴胺和腺苷3',5'-单磷酸调节的磷蛋白）在接受多巴胺输入的神经元中含量丰富。PKA的激活以及随后苏氨酸上DARPP-32的磷酸化是对多巴胺作用于D1样受体的反应。多巴胺与D2样受体的相互作用会抑制PKA的激活，激活蛋白磷酸酶2B，并随后使DARPP-32去磷酸化。除多巴胺以外的神经递质也可能刺激DARPP-32的磷酸化或去磷酸化。磷酸化的DARPP-32是PP-1的强效抑制剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DARPP-32 抗体 (H-3) 参考文献:

  1. L-氨基酸脱羧酶和DARPP-32在肾脏中的意义。  |  Aperia, A., et al. 1990. Am J Hypertens. 3: 11S-13S. PMID: 2166532
  2. DARPP-32作为大鼠脑中D-1多巴胺感受细胞的标记物：产前发育和下丘脑基底神经胶质细胞（坦尼细胞）中的存在。  |  Hökfelt, T., et al. 1988. Adv Exp Med Biol. 235: 65-82. PMID: 2976255
  3. DARPP-32 和 t-DARPP 通过调节 IKKα 依赖性细胞迁移促进非小细胞肺癌的生长。  |  Alam, SK., et al. 2018. Commun Biol. 1: 43. PMID: 29782621
  4. DARPP-32 对 IGF1R 的激活可促进胃癌细胞中 STAT3 信号的传递。  |  Zhu, S., et al. 2019. Oncogene. 38: 5805-5816. PMID: 31235784
  5. 通过调节DARPP-32的表达和信号通路，揭示乳腺癌转录组的复杂性。  |  Saidy, B., et al. 2023. Sci Rep. 13: 21163. PMID: 38036593
  6. Darpp-32受多巴胺调节，是发育中的端脑中GABA能神经元形成所必需的。  |  Souza, BR., et al. 2024. Prog Neuropsychopharmacol Biol Psychiatry. 134: 111060. PMID: 38906412
  7. DARPP-32是一种多巴胺调控的神经元磷蛋白，是蛋白磷酸酶-1的强效阻抑剂。  |  Hemmings, HC., et al. Nature. 310: 503-5. PMID: 6087160
  8. 多巴胺和腺苷-3':5'-单磷酸调控的富含于多巴胺支配的脑区的磷蛋白 DARPP-32。I. 在大鼠大脑中的区域和细胞分布。  |  Walaas, SI. and Greengard, P. 1984. J Neurosci. 4: 84-98. PMID: 6319627
  9. 多巴胺和腺苷 3'：5'-单磷酸调控的神经磷酸蛋白 DARPP-32。I. 磷酸化苏氨酸周围的氨基酸序列。  |  Hemmings, HC., et al. 1984. J Biol Chem. 259: 14486-90. PMID: 6501302
  10. 多巴胺对 DARPP-32 磷酸化的双向调节。  |  Nishi, A., et al. 1997. J Neurosci. 17: 8147-55. PMID: 9334390
  11. DARPP-32/蛋白磷酸酶-1级联：信号整合模型。  |  Greengard, P., et al. 1998. Brain Res Brain Res Rev. 26: 274-84. PMID: 9651542
  12. DARPP-32：多巴胺能神经传递功效的调节器。  |  Fienberg, AA., et al. 1998. Science. 281: 838-42. PMID: 9694658

订购信息

产品名称产品编号规格价格数量收藏夹

DARPP-32 抗体 (H-3)

sc-271111
200 µg/ml
$316.00

DARPP-32 (H-3): m-IgG Fc BP-HRP 套装

sc-529123
200 µg Ab; 10 µg BP
$354.00

DARPP-32 (H-3): m-IgGκ BP-HRP 套装

sc-521851
200 µg Ab, 40 µg BP
$354.00

DARPP-32 抗体 (H-3) AC

sc-271111 AC
500 µg/ml, 25% agarose
$416.00

DARPP-32 抗体 (H-3) HRP

sc-271111 HRP
200 µg/ml
$316.00

DARPP-32 抗体 (H-3) FITC

sc-271111 FITC
200 µg/ml
$330.00

DARPP-32 抗体 (H-3) PE

sc-271111 PE
200 µg/ml
$343.00

DARPP-32 抗体 (H-3) Alexa Fluor® 488

sc-271111 AF488
200 µg/ml
$357.00

DARPP-32 抗体 (H-3) Alexa Fluor® 546

sc-271111 AF546
200 µg/ml
$357.00

DARPP-32 抗体 (H-3) Alexa Fluor® 594

sc-271111 AF594
200 µg/ml
$357.00

DARPP-32 抗体 (H-3) Alexa Fluor® 647

sc-271111 AF647
200 µg/ml
$357.00

DARPP-32 抗体 (H-3) Alexa Fluor® 680

sc-271111 AF680
200 µg/ml
$357.00

DARPP-32 抗体 (H-3) Alexa Fluor® 790

sc-271111 AF790
200 µg/ml
$357.00

DARPP-32 (H-3) 中和勝肽

sc-271111 P
100 µg/0.5 ml
$68.00

Can This antibody reacts with full-length DARPP-32 and truncated DARPP-32?? or reacts both of them? Thank you.

Asked by: songneuron
Thank you for your question. This antibody reacts with full length DARPP-32. According to Uniprot (see link below), the truncated DARPP-32 isoform (also known as t-DARPP) is missing amino acids 1-36. This monoclonal antibody is raised against amino acids 2-34 at the N-terminus of DARPP-32 of human origin, so it would not be expected to detect the truncated isoform.
Answered by: Technical Support
Date published: 2020-08-06

What is the recommended antigen retrieval method and incubation period for this antibody for IHC (mouse paraffin embedded sections)?

Asked by: Ashutosh
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) plus heat treatment for antigen retrieval with this antibody. We recommend incubating with the primary antibody for 30 minutes at room temperature, or overnight at 4 degrees C. A link to our recommended protocol for IHC(P) is provided below for more detailed information. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-11-08

What is the light chain of DARPP-32 (H-3): sc-271111 ?

Asked by: cjMara
DARPP-32 (H-3) is a mouse monoclonal IgG1 with kappa light chain.
Answered by: Technical Support 13
Date published: 2017-01-11

Does DARPP-32 (H-3) work for chicken?

Asked by: cjMara
Yes, this antibody is predicted to cross-react with DARPP-32 of chicken origin based on sequence identity shared with that species.
Answered by: Technical Support 13
Date published: 2017-05-16
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Rated 5 out of 5 by from good for immunostainingno background, very sensitive in striatal tissue from mouse brain
Date published: 2021-07-30
Rated 5 out of 5 by from マウス脳凍結切片の免疫染色に素晴らしくワークマウス脳凍結切片を抗原賦活化処理（クエン酸バッファー:MW処理5min)後 に対して100倍希釈（もっと希釈しても良い）で染色するととてもよく染まります。
Date published: 2020-10-02
Rated 5 out of 5 by from Great immunoreactivityI have used this antibody to detect DARPP-32 protein the striatum of mouse. It worked very well at 1:500 in WB analysis.
Date published: 2018-07-17
Rated 5 out of 5 by from Excellent antibody for IHC and WB applications.Gives clean bands on WB and very robust signal in IHC (even with paraffin sections)
Date published: 2018-06-13
Rated 5 out of 5 by from DARPP32 Immunofluorescence stain-mouse brainIt works very well and can be combined with secondary m-IgGκ BP-CFL 488 antibody. Application: Immunofluorescence (Frozen mouse brain sections); Fixative: 4% Paraformaldehyde; Permeabilization 0.3% Triton X-100 in PBS; Incubation time overnight incubation at 4C; Dilution 1:200. Secondary antibody: m-IgGκ BP-CFL 488 (dilution 1:500, incubation 2h at room temperature).
Date published: 2017-10-11
Rated 5 out of 5 by from DARPP-32 AntibodyThis antibody worked well for total DARPP-32 detection and quantification in mouse ES cells. It gave a specific, sharp band at the expected 32 Kda.
Date published: 2017-10-03
Rated 5 out of 5 by from 功能很出色上半年申请的该抗体，用于westernblot实验，样品为N2A细胞，上样量为50 ug，曝光结果较好。
Date published: 2017-09-27
Rated 5 out of 5 by from Immunofluorescence stain on the zebra finch brainThis antibody provided bright and seemingly specific stain in the zebra finch brain. We have not run Western blots or other confirmation procedure, but the stain is very intense in the striatum as expected. Details: - 4% paraformaldehyde-perfused zebra finch - 0.3% Triton permeabilization - DARPP-32 antibody at 1:500 concentration
Date published: 2017-09-15
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DARPP-32 抗体 (H-3) is rated 4.9 out of 5 by 15.
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