Date published: 2026-10-17
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cytochrome c Antibody (6H2) - Western Blotting - Image 380260
细胞色素 c 抗体 (6H2): sc-13561. PC-12 (A), C6 (B), Neuro-2A (C), RAW 264. 7 (D), SK-N-SH (E) 和 Hep G2 (F) 全细胞裂解液中细胞色素 c 表达的 Western 印迹分析.
细胞色素 c 抗体 (6H2): sc-13561. PC-12 (A), C6 (B), Neuro-2A (C), RAW 264. 7 (D), SK-N-SH (E) 和 Hep G2 (F) 全细胞裂解液中细胞色素 c 表达的 Western 印迹分析.

cytochrome c 抗体 (6H2): sc-13561

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寫評論提問
说明书
  • cytochrome c抗体(6H2)是小鼠单克隆IgG1 κ, 在162篇文献中引用，规格为200 µg/ml
  • 针对全长大鼠蛋白的
  • 抗-cytochrome c 抗体 (6H2) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 native form of cytochrome c
  • cytochrome c抗体（6H2）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是cytochrome c Antibody (6H2) for WB applications. 的首选辅助检测试剂，这些试剂现与cytochrome c Antibody (6H2) 打包提供（请参阅下面的订购信息）。
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関連項目

额外Cytochrome抗体，包括cytochrome b5 type B

细胞色素c抗体（6H2）是一种IgG1 κ小鼠单克隆细胞色素c抗体，可通过WB、IP、IF、FCM和ELISA检测小鼠、大鼠和人源的细胞色素c的天然形式。细胞色素c抗体（6H2）有偶联和非偶联抗细胞色素c抗体两种形式。细胞色素c是一种特征明确的移动电子传递蛋白，对于所有有氧生物的能量转换至关重要。在哺乳动物细胞中，这种高度保守的蛋白通常定位于线粒体膜间隙。最近的研究已经确定胞质细胞色素c是激活凋亡所必需的因素。在凋亡过程中，细胞色素c从线粒体膜转位到胞质溶胶中，在那里它需要激活caspase-3（CPP32）。已经显示Bcl-2的过表达可以防止细胞色素c的转位，从而阻断凋亡过程。已经显示Bax的过表达可以诱导细胞色素c的释放并诱导细胞死亡。据认为，从线粒体释放的细胞色素c触发凋亡级联反应，其中Apaf-1以细胞色素c依赖的方式与Apaf-3（caspase-9）结合，导致caspase-9切割caspase-3。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cytochrome c 抗体 (6H2) 参考文献:

  1. 线粒体中的细胞色素c信号复合物。  |  Díaz-Moreno, I., et al. 2011. Eur Biophys J. 40: 1301-15. PMID: 22086608
  2. 灵长类动物细胞色素c和细胞色素c氧化酶的进化。  |  Pierron, D., et al. 2012. Adv Exp Med Biol. 748: 185-213. PMID: 22729859
  3. 细胞色素c与心磷脂相互作用后的结构转变。  |  Muenzner, J. and Pletneva, EV. 2014. Chem Phys Lipids. 179: 57-63. PMID: 24252639
  4. 细胞色素c与PCB醌类代谢物的结合物。  |  Li, M., et al. 2016. Environ Sci Pollut Res Int. 23: 2148-59. PMID: 26062463
  5. 细胞色素C在脓毒症休克患者中的作用。  |  Andersen, LW., et al. 2016. Shock. 45: 512-7. PMID: 26717109
  6. 细胞色素c-封端荧光金纳米团簇：活细胞成像和细胞色素c的递送。  |  Chattoraj, S., et al. 2016. Chemphyschem. 17: 2088-95. PMID: 27028215
  7. 免疫细胞化学分析细胞色素c释放。  |  Crowley, LC., et al. 2016. Cold Spring Harb Protoc. 2016: PMID: 27934681
  8. 细胞色素 c 负向调节 NLRP3 炎症小体  |  Shi, CS. and Kehrl, JH. 2016. PLoS One. 11: e0167636. PMID: 28030552
  9. 细胞色素c和细胞色素c氧化酶的晶体学研究。  |  Tsukihara, T. 2022. J Biochem. 171: 13-15. PMID: 34697634
  10. 细胞色素c对谷胱甘肽氧化的表征。  |  Csomó, KB., et al. 2022. J Bioenerg Biomembr. 54: 1-8. PMID: 34893948

订购信息

产品名称产品编号规格价格数量收藏夹

cytochrome c 抗体 (6H2)

sc-13561
200 µg/ml
$322.00

cytochrome c (6H2): m-IgG Fc BP-HRP 套装

sc-526560
200 µg Ab; 10 µg BP
$361.00

cytochrome c (6H2): m-IgG1 BP-HRP 套装

sc-531933
200 µg Ab; 20 µg BP
$361.00

cytochrome c 抗体 (6H2) AC

sc-13561 AC
500 µg/ml, 25% agarose
$424.00

cytochrome c 抗体 (6H2) FITC

sc-13561 FITC
200 µg/ml
$336.00

cytochrome c 抗体 (6H2) PE

sc-13561 PE
200 µg/ml
$349.00

cytochrome c 抗体 (6H2) Alexa Fluor® 488

sc-13561 AF488
200 µg/ml
$364.00

cytochrome c 抗体 (6H2) Alexa Fluor® 546

sc-13561 AF546
200 µg/ml
$364.00

cytochrome c 抗体 (6H2) Alexa Fluor® 594

sc-13561 AF594
200 µg/ml
$364.00

cytochrome c 抗体 (6H2) Alexa Fluor® 647

sc-13561 AF647
200 µg/ml
$364.00

cytochrome c 抗体 (6H2) Alexa Fluor® 680

sc-13561 AF680
200 µg/ml
$364.00

cytochrome c 抗体 (6H2) Alexa Fluor® 790

sc-13561 AF790
200 µg/ml
$364.00

cytochrome c 抗体 (6H2) Alexa Fluor® 405

sc-13561 AF405
200 µg/ml
$364.00

Can the 6H2 clone be used for immunohistochemistry on mouse tissue?

Asked by: cjMara
The 6H2 clone is not recommended for IHC(P). A much better alternative for detection of cytochrome c in formalin-fixed paraffin-embedded tissue sections is our alternate clone, cytochrome c Antibody (A-8): sc-13156.
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by xinji18 from Great featureI brought 2 weeks age, it works well for western, highly recommended
Date published: 2017-10-16
Rated 5 out of 5 by Bill28 from OutstandingGreat western blot data; strong signal; also clear IF and FCM results
Date published: 2017-01-26
Rated 5 out of 5 by DefinitelyNotMatt from Great Antibody for IFThis antibody is well-cited for a variety of applications including WB, IP, IF, and even Flow and ELISA. Strong, clear signal with little to no background. Beautiful florescence.
Date published: 2017-01-06
Rated 4 out of 5 by LM from Produced robust intracellular FCM data forProduced robust intracellular FCM data for fixed and permeabilized HL-60 cells. -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by HF from Worked well for ChIPWorked well for ChIP - working with rat pituitary somatolactotrophs. -SCBT Publication Review
Date published: 2015-04-25
Rated 4 out of 5 by SF from Produced clear immunofluorescence cytoplasmicProduced clear immunofluorescence cytoplasmic staining in methanol-fixed K-562 cells. -SCBT QC
Date published: 2015-03-21
Rated 3 out of 5 by Xing from I have tried the antibodyI have tried the antibody, it works, but very weak. You have to expose very long time and load huge amount protein.
Date published: 2015-03-18
Rated 4 out of 5 by TS from Robust intracellular FCM data of fixed andRobust intracellular FCM data of fixed and permeabilized K-562 cells. -SCBT QC
Date published: 2014-09-28
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cytochrome c 抗体 (6H2) is rated 4.2 out of 5 by 9.
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