Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CYPOR Double Nickase Plasmid (m): sc-422345-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYPOR Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CYPOR Double Nickase Plasmid (m) and CYPOR Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Por. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CYPOR Antibody (F-10): sc-25270
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYPOR Double Nickase Plasmid (m) | sc-422345-NIC | 20 µg | $410.00 |
Mouse Por encodes cytochrome P450 oxidoreductase (CYPOR), a flavoprotein that transfers electrons from NADPH to microsomal cytochrome P450 enzymes and other redox partners. CYPOR is central to oxidative metabolism, supporting pathways involved in xenobiotic clearance, steroid and lipid biosynthesis, and redox homeostasis within the endoplasmic reticulum. Altered POR/CYPOR activity can perturb P450-dependent metabolic networks, influencing susceptibility to drug–gene interactions, oxidative stress responses, and endocrine-related phenotypes. Por is therefore widely studied in hepatic metabolism, developmental biology, and systems-level regulation of detoxification pathways.
CYPOR Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Por locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Por. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Por function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Por-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.