Date published: 2025-9-7

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cyclin A 抗体 (H-3): sc-271645

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说明书
  • cyclin A抗体(H-3)是小鼠单克隆IgG1 κ, 在33篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长cyclin A的氨基酸1-432
  • 抗-cyclin A 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 cyclin A and cyclin A1
  • 抗cyclin A抗体(H-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271645 X, 200 µg/0.1 ml)
  • 关于如何获取cyclin A (H-3): sc-271645免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是cyclin A Antibody (H-3) for WB applications. 的首选辅助检测试剂,这些试剂现与cyclin A Antibody (H-3) 打包提供(请参阅下面的订购信息)。

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関連項目

cyclin A抗体(H-3)是一种IgG1 κ小鼠单克隆cyclin A抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源cyclin A和cyclin A1。cyclin A抗体(H-3)既可作为非偶联抗cyclin A抗体形式使用,也可作为多种偶联形式的抗cyclin A抗体使用,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。调节蛋白家族(称为细胞周期蛋白)在真核细胞周期调节中的关键作用已得到充分证实。表征最好的细胞周期蛋白复合物是有丝分裂细胞周期蛋白B/Cdc2 p34激酶,这是成熟促进因子的活性成分。细胞周期蛋白A在细胞周期中先于细胞周期蛋白B积累,似乎参与S期的控制,并已显示与细胞周期蛋白依赖性激酶-2(Cdk2)相关联。此外,细胞周期蛋白A已被证实参与细胞转化,并与E1A、转录因子DRTF1和E2F以及视网膜母细胞瘤蛋白p110形成复合物。第二种形式的细胞周期蛋白A被称为细胞周期蛋白A1,因为它与非洲爪蟾细胞周期蛋白A1具有高度序列同源性,在生殖细胞中的表达最高。有人提出,细胞周期蛋白A1可以与Cdk2、p39和Cdc2 p34结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin A 抗体 (H-3)

sc-271645
200 µg/ml
$316.00

cyclin A (H-3): m-IgG Fc BP-HRP 套装

sc-527272
200 µg Ab; 10 µg BP
$354.00

cyclin A (H-3): m-IgG1 BP-HRP 套装

sc-532645
200 µg Ab; 20 µg BP
$354.00

cyclin A 抗体 (H-3) X

sc-271645 X
200 µg/0.1 ml
$316.00

cyclin A 抗体 (H-3) AC

sc-271645 AC
500 µg/ml, 25% agarose
$416.00

cyclin A 抗体 (H-3) HRP

sc-271645 HRP
200 µg/ml
$316.00

cyclin A 抗体 (H-3) FITC

sc-271645 FITC
200 µg/ml
$330.00

cyclin A 抗体 (H-3) PE

sc-271645 PE
200 µg/ml
$343.00

cyclin A 抗体 (H-3) Alexa Fluor® 488

sc-271645 AF488
200 µg/ml
$357.00

cyclin A 抗体 (H-3) Alexa Fluor® 546

sc-271645 AF546
200 µg/ml
$357.00

cyclin A 抗体 (H-3) Alexa Fluor® 594

sc-271645 AF594
200 µg/ml
$357.00

cyclin A 抗体 (H-3) Alexa Fluor® 647

sc-271645 AF647
200 µg/ml
$357.00

cyclin A 抗体 (H-3) Alexa Fluor® 680

sc-271645 AF680
200 µg/ml
$357.00

cyclin A 抗体 (H-3) Alexa Fluor® 790

sc-271645 AF790
200 µg/ml
$357.00

Can the X form of this antibody be used for applications other than ChIP and EMSA, such as Western Blot or IF?

Asked by: AbPolly
Thank you for your question. Yes, the X form of this antibody can be used in applications such as Western Blot or IF, the dilution factor just needs to be adjusted as it is ten times as concentrated as the standard form.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 5 out of 5 by from 免疫沈降で使用Immunoprecipitationで使用。5ul/sampleで用いた。良好に免疫沈降することができた。
Date published: 2020-06-19
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysatesTested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Presence of CcnA protein in this cell line was previously confirmed using discontinued product sc-596
Date published: 2019-05-22
Rated 1 out of 5 by from no signal with HUVECI got this sample 6 months ago to replace sc-596 for cyclin A with WB. With 35ug of HUVEC samples, I got NO signal after 5 minute exposure (primary 1:350, secondary 1:2000).
Date published: 2018-05-02
Rated 5 out of 5 by from Good ab for WBworks well for western blotting with samples from human cultured cells
Date published: 2017-11-21
Rated 5 out of 5 by from Sehr gute ergebnisse WB 1-300 dilution !I ordered 1 month ago, this cyclin a seems the best for mouse samples, I was working with primary cells isolated from young and old mice.
Date published: 2017-11-15
Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Nice bandsTested several samples with cyclin A Antibody (H-3) and observed strong and specific band
Date published: 2017-01-19
Rated 5 out of 5 by from Works well for IF in mouse (NIH3T3)Works well for IF in mouse (NIH3T3), publishable data. -SCBT Publication Review
Date published: 2015-03-05
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cyclin A 抗体 (H-3) is rated 4.1 out of 5 by 9.
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