Date published: 2025-9-8

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CSP 抗体 (H-3): sc-137128

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说明书
  • CSP 抗体 H-3 是小鼠单克隆 IgG1 (kappa轻链),CSP抗体,规格为200 µg/ml
  • 针对氨基酸71-198,在CSP的human起源的C-terminus处进行映射
  • CSP 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CSP isoforms 1 and 2 and β-CSP; 也和以下物种反应,包括: equine
  • 抗CSP抗体(H-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CSP (H-3): sc-137128免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是CSP Antibody (H-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CSP Antibody (H-3) 打包提供(请参阅下面的订购信息)。

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    関連項目

    CSP抗体(H-3)是一种IgG1 κ小鼠单克隆CSP抗体(也称为CSP抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CSP蛋白。CSP抗体(H-3)既有非偶联的抗CSP抗体形式,也有多种偶联形式的抗CSP抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。半胱氨酸串蛋白(CSPs)是与突触囊泡相关的分泌囊泡蛋白,参与突触囊泡的Ca2+调节胞吐作用和神经内分泌及内分泌细胞类型中突触前跨膜钙流的调节。CSP包含一个与HSP 70/HSC 70伴侣ATP酶结合的J结构域和一个膜靶向的棕榈酰化半胱氨酸丰富的串区。CSPs可能在突触中充当分子伴侣,并介导囊泡胞吐机制组分的构象折叠。CSP通过与受体偶联的三聚体GTP结合蛋白(G蛋白)和N型Ca2+通道的相互作用参与神经传递的精细调节。已经描述了CSP的两种变体:CSP1;以及31个氨基酸的C末端截断的同种型,CSP2。胰岛素瘤细胞的亚细胞分馏显示CSP1在颗粒级分中,而膜和胞质级分主要包含CSP2。这些级分还含有其他蛋白质,大概是CSP二聚体。此外,在各种哺乳动物细胞系(包括大鼠大脑)中,CSP1的表达优于CSP2的表达。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    CSP 抗体 (H-3) 参考文献:

    1. 半胱氨酸串联蛋白在胰岛素外吞过程中的功能突变分析  |  Zhang, H., et al. 1999. J Cell Sci. 112 (Pt 9): 1345-51. PMID: 10194413
    2. 半胱氨酸弦蛋白:突触的伴侣。  |  Chamberlain, LH. and Burgoyne, RD. 2000. J Neurochem. 74: 1781-9. PMID: 10800920
    3. 半胱氨酸串联蛋白调控 G 蛋白对 N 型钙通道的调节。  |  Magga, JM., et al. 2000. Neuron. 28: 195-204. PMID: 11086994
    4. 乳腺上皮细胞中半胱氨酸字符串蛋白的表达。  |  Gleave, TL., et al. 2001. Pflugers Arch. 441: 639-49. PMID: 11294245
    5. 糖尿病 GK 大鼠胰岛外泌蛋白表达下调。  |  Zhang, W., et al. 2002. Biochem Biophys Res Commun. 291: 1038-44. PMID: 11866470
    6. 半胱氨酸串联蛋白在调节外吞过程中直接发挥作用。  |  Chamberlain, LH. and Burgoyne, RD. 1998. Mol Biol Cell. 9: 2259-67. PMID: 9693380
    7. 半胱氨酸串联蛋白(CSP)是一种胰岛素分泌颗粒相关蛋白,可调节β细胞的外泌。  |  Brown, H., et al. 1998. EMBO J. 17: 5048-58. PMID: 9724640

    订购信息

    产品名称产品编号规格价格数量收藏夹

    CSP 抗体 (H-3)

    sc-137128
    200 µg/ml
    $316.00

    CSP (H-3): m-IgG Fc BP-HRP 套装

    sc-528864
    200 µg Ab; 10 µg BP
    $354.00

    CSP (H-3): m-IgGκ BP-HRP 套装

    sc-521432
    200 µg Ab, 40 µg BP
    $354.00

    CSP (H-3): m-IgG1 BP-HRP 套装

    sc-543176
    200 µg Ab; 20 µg BP
    $354.00

    CSP 抗体 (H-3) AC

    sc-137128 AC
    500 µg/ml, 25% agarose
    $416.00

    CSP 抗体 (H-3) HRP

    sc-137128 HRP
    200 µg/ml
    $316.00

    CSP 抗体 (H-3) FITC

    sc-137128 FITC
    200 µg/ml
    $330.00

    CSP 抗体 (H-3) PE

    sc-137128 PE
    200 µg/ml
    $343.00

    CSP 抗体 (H-3) Alexa Fluor® 488

    sc-137128 AF488
    200 µg/ml
    $357.00

    CSP 抗体 (H-3) Alexa Fluor® 546

    sc-137128 AF546
    200 µg/ml
    $357.00

    CSP 抗体 (H-3) Alexa Fluor® 594

    sc-137128 AF594
    200 µg/ml
    $357.00

    CSP 抗体 (H-3) Alexa Fluor® 647

    sc-137128 AF647
    200 µg/ml
    $357.00

    CSP 抗体 (H-3) Alexa Fluor® 680

    sc-137128 AF680
    200 µg/ml
    $357.00

    CSP 抗体 (H-3) Alexa Fluor® 790

    sc-137128 AF790
    200 µg/ml
    $357.00

    Why is the size of western blotting band 34 kDa ? In database, CSP/Dnajc5 protein size is 22 kDa. is this band really CSP? How can you sure, even the size is completely different ?

    Asked by: JKkim
    Thank you for your question. We have observed CSP in different tissue extracts at approximately 35 kDa using this antibody, CSP (H-3): sc-137128, as well as another antibody to the same target with a different epitope, CSP (16): sc-136468, (https://www.scbt.com/scbt/product/csp-antibody-16). This observed molecular weight is also consistent with a reference for CSP reported in the literature, on NCBI PubMed, PMID: 7666205.
    Answered by: Technical Support
    Date published: 2017-09-21

    I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CSP (H-3): sc-137128?

    Asked by: DefinitelyNotMatt
    We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 4 out of 5 by from Excellent for western blotting of brain extractsBought this antibody a month ago and it has performed very well for western blotting mouse brain extracts
    Date published: 2020-06-03
    Rated 5 out of 5 by from Great!Produced nice Western blot data of CSP expression in C2C12 cell lysate.
    Date published: 2016-10-27
    Rated 5 out of 5 by from Produced positive Western Blot data of CSPProduced positive Western Blot data of CSP expression in non transfected and mouse CSP transfected 293T whole cell lysates. -SCBT QC
    Date published: 2015-01-12
    Rated 5 out of 5 by from Great Western Blot data of CSP expressionGreat Western Blot data of CSP expression in rat cerebellum and rat brain tissue extracts. -SCBT QC
    Date published: 2014-06-14
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    CSP 抗体 (H-3) is rated 4.8 out of 5 by 4.
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