Date published: 2025-9-9
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CRM1 Antibody (H-7) - Western Blotting - Image 65624
CRM1 抗体 (H-7): sc-74455. HeLa (A), A-431 (B), K-562 (C) 和 Jurkat (D) 核提取物中 CRM1 表达的 Western 印迹分析.
CRM1 抗体 (H-7): sc-74455. HeLa (A), A-431 (B), K-562 (C) 和 Jurkat (D) 核提取物中 CRM1 表达的 Western 印迹分析.

CRM1 抗体 (H-7): sc-74455

4.8(8)
寫評論提問

说明书
  • CRM1抗体(H-7)是小鼠单克隆IgG1 κ, 在7篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CRM1的氨基酸772-1071
  • 抗-CRM1 抗体 (H-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CRM1
  • 抗CRM1抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CRM1 (H-7): sc-74455的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CRM1 抗体 (H-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CRM1 抗体 (H-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CRM1抗体（H-7）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的CRM1，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）。CRM1 (H-7) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 结合物。CRM1 也称为出口蛋白 1，在蛋白质和 RNA 的核出口中起着至关重要的作用，促进分子从细胞核向细胞质的运输。这一过程通过确保参与各种细胞过程的蛋白质定位正确，从而维持细胞功能并调节基因表达。CRM1能够识别含有核出口信号（NES）的蛋白质，从而选择性地出口这些蛋白质，这对于细胞信号传导和应激反应等过程至关重要。CRM1还与其它几种蛋白质相互作用，包括核转运蛋白α1亚基和β1亚基，它们是核运输机制的关键参与者。了解CRM1的功能和相互作用有助于阐明核质运输的复杂性以及其在癌症和病毒感染等各种疾病中的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CRM1 抗体 (H-7)

sc-74455
200 µg/ml
$316.00

CRM1 (H-7): m-IgG Fc BP-HRP 套装

sc-527021
200 µg Ab; 10 µg BP
$354.00

CRM1 (H-7): m-IgG1 BP-HRP 套装

sc-532394
200 µg Ab; 20 µg BP
$354.00

CRM1 抗体 (H-7) AC

sc-74455 AC
500 µg/ml, 25% agarose
$416.00

CRM1 抗体 (H-7) HRP

sc-74455 HRP
200 µg/ml
$316.00

CRM1 抗体 (H-7) FITC

sc-74455 FITC
200 µg/ml
$330.00

CRM1 抗体 (H-7) PE

sc-74455 PE
200 µg/ml
$343.00

CRM1 抗体 (H-7) Alexa Fluor® 488

sc-74455 AF488
200 µg/ml
$357.00

CRM1 抗体 (H-7) Alexa Fluor® 546

sc-74455 AF546
200 µg/ml
$357.00

CRM1 抗体 (H-7) Alexa Fluor® 594

sc-74455 AF594
200 µg/ml
$357.00

CRM1 抗体 (H-7) Alexa Fluor® 647

sc-74455 AF647
200 µg/ml
$357.00

CRM1 抗体 (H-7) Alexa Fluor® 680

sc-74455 AF680
200 µg/ml
$357.00

CRM1 抗体 (H-7) Alexa Fluor® 790

sc-74455 AF790
200 µg/ml
$357.00

I am using CRM1 (H-7): sc-74455 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 功能很出色我一个月前购买的此产品，体验过程还不错，以后根据试验进度来决定下一步计划。。。。。。。。。。。。。。
Date published: 2020-01-03
Rated 5 out of 5 by from Clean and strongUsed CRM1 Antibody (H-7) by IHCP and WB. WB results were strong with little to no background and IHCP stain was specific
Date published: 2017-01-25
Rated 4 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-11-25
Rated 5 out of 5 by from We used this antibody in mouse tissues andWe used this antibody in mouse tissues and worked well at 1:500 dilution.
Date published: 2015-09-17
Rated 4 out of 5 by from Shows expression of CRM1 in HeLaShows expression of CRM1 in HeLa, A-431, K-562 and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Band detected at expected MW in 293T wholeBand detected at expected MW in 293T whole cell lysate. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Positive WB detection in HeLa nuclear extractPositive WB detection in HeLa nuclear extract, and NIH/3T3 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Great immunofluorescence staining shows nuclearGreat immunofluorescence staining shows nuclear localization on methanol fixed HeLa cells. -SCBT QC
Date published: 2013-10-01
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CRM1 抗体 (H-7) is rated 4.8 out of 5 by 8.
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