Date published: 2025-9-24
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COX2 Antibody (D-5) - Western Blotting - Image 319392
COX2 抗体 (D-5): sc-514489. 在 HeLa (A), THP-1 (B), MCF7 (C), K-562 (D) 和 Jurkat (E) 全细胞裂解液中 COX2 表达的 Western 印迹分析.
COX2 抗体 (D-5): sc-514489. 在 HeLa (A), THP-1 (B), MCF7 (C), K-562 (D) 和 Jurkat (E) 全细胞裂解液中 COX2 表达的 Western 印迹分析.

COX2 抗体 (D-5): sc-514489

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寫評論提問

说明书
  • COX2 抗体 D-5 是小鼠单克隆 IgM κ，COX2抗体, 在72篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的COX2的内部区域内的氨基酸169-192之间
  • 推荐用于 mouse, rat 和 human 来源的COX2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取COX2 (D-5): sc-514489的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成COX2 抗体 (D-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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说明书与实验方案
研究信息

関連項目

此外 Cytochrome c Oxidase 抗体 包括 COX15, COX17, COX2, COX4, COX4I2, COX4NB, COX5a, COX5b, COX6b1和COX6c

COX2抗体（D-5）是一种IgM κ小鼠单克隆COX2抗体（也被指定为MTCO2抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组化（IHC(P)）和酶联免疫吸附试验（ELISA）等方法检测来自小鼠、大鼠和人类的COX2蛋白。COX2抗体（D-5）为非偶联形式的抗COX2抗体。细胞色素c氧化酶亚基II（COX2），也被称为COII、MTCO2或氧化磷酸化（OxPhos）复合物IV亚基II，是呼吸复合物IV的三个线粒体DNA（mtDNA）编码亚基（MTCO1-3）之一。细胞色素c氧化酶是一种由13个亚基组成的异寡聚酶，位于线粒体内膜上，是电子传递链的末端酶复合物。复合物IV催化分子氧还原为水。释放的能量用于将质子跨线粒体内膜运输。由此产生的电化学梯度对于ATP的合成是必需的。复合物IV含有13个多肽；COX1、COX2和COX3（MTCO1-3）构成催化核心，由mtDNA编码，而亚基IV、Va、Vb、VIa、VIb、VIc、VIIa、VIIb、VIIc和VIII则由核编码。COX2的缺陷与肿瘤形成有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COX2 抗体 (D-5) 参考文献:

  1. 健康和疾病中的细胞色素氧化酶。  |  Barrientos, A., et al. 2002. Gene. 286: 53-63. PMID: 11943460
  2. 细胞色素 C 氧化酶中保守精氨酸对质子和电子转移的作用。  |  Qian, J., et al. 2004. Biochemistry. 43: 5748-56. PMID: 15134449
  3. 表皮生长因子受体上 Y845 的磷酸化介导了与线粒体蛋白细胞色素 c 氧化酶亚基 II 的结合。  |  Boerner, JL., et al. 2004. Mol Cell Biol. 24: 7059-71. PMID: 15282306
  4. 发育中大鼠大脑中细胞色素 C 氧化酶亚基 III 和 IV 的表达分析  |  Cannino, G., et al. 2004. Neuroscience. 128: 91-8. PMID: 15450356
  5. 用十二烷基硫酸钠-凝胶电泳法将哺乳动物细胞色素 c 氧化酶分离成 13 种多肽。  |  Kadenbach, B., et al. 1983. Anal Biochem. 129: 517-21. PMID: 6303162
  6. 细胞色素 c 氧化酶的结构。  |  Capaldi, RA., et al. 1983. Biochim Biophys Acta. 726: 135-48. PMID: 6307356

订购信息

产品名称产品编号规格价格数量收藏夹

COX2 抗体 (D-5)

sc-514489
200 µg/ml
$316.00

COX2 (D-5) 中和勝肽

sc-514489 P
100 µg/0.5 ml
$68.00

请问这个抗体可以做冰冻切片的免疫组化么？

Asked by: libing
感谢您的提问，sc-514489 COX2 (D-5)推荐做冰冻切片的免疫组化实验。 Thank you for your question，sc-51449 COX2 (D-5) is recommended for detection of COX2 by IF on frozen tissue.
Answered by: Technical Support 8
Date published: 2018-12-21

Can this antibody be used to detect COX2 of mouse origin?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-01-17
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Rated 4 out of 5 by from Antibody worked well for IHCIn human samples, IHC worked well in paraffin-embedded tissues.
Date published: 2025-06-06
Rated 5 out of 5 by from Excellent antibodyVery good to use. The dilution ratio for WB is 1:200
Date published: 2024-10-15
Rated 4 out of 5 by from Good antibodyMultiple bands were detected but still expected size band was detected.
Date published: 2021-12-03
Rated 5 out of 5 by from Excellent in western blottingWestern blotting Hepg2 cell lysate dilution 1:1000 Strong and specific band at the expected molecular weight
Date published: 2020-06-18
Rated 1 out of 5 by from Wrong molecular markers!The size of CoxII is 75kDa. The image on this page is misleading. It shows the band around 25kDa. Technicall error? Otherwise am I wrong here?
Date published: 2019-10-22
Rated 4 out of 5 by from Works nicelyWe used it 1:250 on HeLa lysates and detected a clear band of expected molecular weight.
Date published: 2018-10-25
Rated 5 out of 5 by from Good ProductThe antibody used in this paper worked well with brain tissue extract at 1:500 dilution.
Date published: 2018-08-29
Rated 5 out of 5 by from Great antibodyThis antibody is highly sensitive for western blotting, with little background.
Date published: 2018-08-06
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COX2 抗体 (D-5) is rated 4.3 out of 5 by 30.
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