Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

COPB 抗体 (D-10): sc-393615

4.7(7)
寫評論提問

说明书
  • COPB 抗体 D-10 是小鼠单克隆 IgG2b κ,COPB抗体, 在14篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于rat物种的COPB的N-terminus的氨基酸2-19之间
  • COPB 抗体 (D-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ,检测 mouse, rat, human 和 来源的COPB; 也和以下物种反应,包括: 和 equine, canine, bovine, porcine and avian
  • 抗COPB抗体(D-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取COPB (D-10): sc-393615免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 COPB 抗体 (D-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与COPB 抗体 (D-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

COPB抗体(D-10)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的COPB蛋白。COPB (D-10) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。COPB蛋白(也称为β-COP)在早期分泌途径中的膜和囊泡运输中起着至关重要的作用,特别是通过非Clathrin包被的囊泡介导蛋白质从内质网到高尔基体的运输。COPB 负责维持细胞内环境稳定,确保蛋白质正确加工并运送到目的地。COPB 充当前高尔基体中间体的标记物,例如囊泡管状簇(VTC),这些中间体对于细胞内的货物组织和运输至关重要。COPB与GIV(Gα i/s相互作用蛋白)相互作用,与Gα i3共同定位在内质网出口和顺式高尔基体囊泡上,这凸显了COPB在囊泡运输调节中的重要性。COPB与高尔基复合体中的Afadin DIL域相互作用蛋白(ADIP)相互作用,这强调了COPB在协调正常细胞功能所需的运输过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COPB 抗体 (D-10) 参考文献:

  1. 非典型蛋白激酶 C 在前高尔基中间体的蛋白质转运过程中发挥着关键作用。  |  Tisdale, EJ., et al. 2003. J Biol Chem. 278: 38015-21. PMID: 12871960
  2. 去泛素化,高尔基体到内质网逆向运输的新角色。  |  Cohen, M., et al. 2003. J Biol Chem. 278: 51989-92. PMID: 14593109
  3. beta-COP 参与了通过高尔基复合体的膜运输。  |  Duden, R., et al. 1991. Trends Cell Biol. 1: 14-9. PMID: 14731804
  4. 哺乳动物 POD-1 的同源物 Coronin 7 定位于高尔基体。  |  Rybakin, V., et al. 2004. FEBS Lett. 573: 161-7. PMID: 15327992
  5. Afadin和α-肌动蛋白结合蛋白ADIP可直接结合外套复合物的一个亚基--β'-COP。  |  Asada, M., et al. 2004. Biochem Biophys Res Commun. 321: 350-4. PMID: 15358183
  6. 鉴定和描述在 COPI, 内质网-高尔基体转运囊泡上发现的新型 Galpha i/s-交互蛋白 GIV。  |  Le-Niculescu, H., et al. 2005. J Biol Chem. 280: 22012-20. PMID: 15749703
  7. 外套膜的体外组装和拆卸。  |  Lowe, M. and Kreis, TE. 1995. J Biol Chem. 270: 31364-71. PMID: 8537409
  8. 在衣壳蛋白的 gamma 亚基中,存在一个单一的地赖氨酸检索基序和 p23 结合位点。  |  Harter, C. and Wieland, FT. 1998. Proc Natl Acad Sci U S A. 95: 11649-54. PMID: 9751720

订购信息

产品名称产品编号规格价格数量收藏夹

COPB 抗体 (D-10)

sc-393615
200 µg/ml
$316.00

COPB (D-10): m-IgGκ BP-HRP 套装

sc-524025
200 µg Ab, 40 µg BP
$354.00

COPB (D-10): m-IgG2b BP-HRP 套装

sc-549150
200 µg Ab; 10 µg BP
$354.00

COPB 抗体 (D-10) AC

sc-393615 AC
500 µg/ml, 25% agarose
$416.00

COPB 抗体 (D-10) HRP

sc-393615 HRP
200 µg/ml
$316.00

COPB 抗体 (D-10) FITC

sc-393615 FITC
200 µg/ml
$330.00

COPB 抗体 (D-10) PE

sc-393615 PE
200 µg/ml
$343.00

COPB 抗体 (D-10) Alexa Fluor® 488

sc-393615 AF488
200 µg/ml
$357.00

COPB 抗体 (D-10) Alexa Fluor® 546

sc-393615 AF546
200 µg/ml
$357.00

COPB 抗体 (D-10) Alexa Fluor® 594

sc-393615 AF594
200 µg/ml
$357.00

COPB 抗体 (D-10) Alexa Fluor® 647

sc-393615 AF647
200 µg/ml
$357.00

COPB 抗体 (D-10) Alexa Fluor® 680

sc-393615 AF680
200 µg/ml
$357.00

COPB 抗体 (D-10) Alexa Fluor® 790

sc-393615 AF790
200 µg/ml
$357.00

COPB (D-10) 中和勝肽

sc-393615 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-393615 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_393615, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 126ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from anti-COPB good featuresI used anti-COPB antibody for IP-WB to detect variation between KO cells with rescue mutant proteins. Results were good enough to see a variation between mutants.
Date published: 2022-08-17
Rated 5 out of 5 by from Perfect IF!I have tested this antibody in IF on human fibroblasts and it showed a strong vesicular signal in the perinuclear region.
Date published: 2018-01-23
Rated 3 out of 5 by from COPB(D-10) mouse monoclonal antibodyworked OK on rat cardiac cardiomyocytes, there was some background though
Date published: 2017-12-12
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from The preferred choice for IHC(P)This monoclonal COPB Antibody (D-10) is the preferred choice for IHC(P) and IF applications. Excellent results!
Date published: 2017-01-31
Rated 5 out of 5 by from Exceptional immunofluorescence Golgi apparatusExceptional immunofluorescence Golgi apparatus and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Good Western Blot data of COPB expressionGood Western Blot data of COPB expression in C6 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2013-07-26
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_393615, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
COPB 抗体 (D-10) is rated 4.7 out of 5 by 7.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_393615, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT