Date published: 2025-9-7

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connexin 40 抗体 (B-3): sc-365107

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说明书
  • connexin 40 抗体 B-3 是小鼠单克隆 IgG1 κ,connexin 40抗体, 在14篇文献中引用,规格为200 µg/ml
  • 免疫human来源connexin 40位于C-terminal细胞质域的231-346氨基酸
  • connexin 40 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 connexin 40
  • 抗connexin 40抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取connexin 40 (B-3): sc-365107免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是connexin 40 Antibody (B-3) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与connexin 40 Antibody (B-3) 打包提供(请参阅下面的订购信息)。

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関連項目

connexin 40抗体(B-3)是一种IgG1 κ小鼠单克隆connexin 40抗体(也称为GJA5抗体、间隙连接蛋白alpha 5抗体或CX40抗体),可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的connexin 40蛋白。connexin 40抗体(B-3)既有非偶联形式的抗connexin 40抗体,也有多种偶联形式的抗connexin 40抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。连接蛋白家族形成称为"连接子"的六聚体复合物,其通过间隙连接促进低分子量蛋白质在细胞间的移动。连接蛋白具有共同的拓扑结构,包含4个跨膜α-螺旋结构域、两个细胞外环、一个细胞质环以及细胞质N端和C端。许多关键的功能差异源于最保守结构域,即跨膜区和细胞外区中的特定氨基酸替换。大约20种连接蛋白异构体中的每一种都会产生具有不同通透性和电化学敏感性的通道;因此,一种连接蛋白通常不能完全替代另一种。因此,许多恶性表型与连接蛋白表达和间隙连接通讯的降低有关,具体取决于所影响的特定连接蛋白。例如,心脏手术后connexin 40的上调可表明对术后房颤的易感性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

connexin 40 抗体 (B-3) 参考文献:

  1. 对家族性心房颤动中附件蛋白 40 的分子分析。  |  Lamarche, J., et al. 2001. Eur Heart J. 22: 1511-2. PMID: 11482925
  2. 附件信道的新问题:生物物理学填补了空白。  |  Harris, AL. 2001. Q Rev Biophys. 34: 325-472. PMID: 11838236
  3. 对心脏间隙连接蛋白 connexin 43 和 connexin 40 的药理调节和不同调控。  |  Dhein, S., et al. 2002. Biol Cell. 94: 409-22. PMID: 12566216
  4. 血管连接蛋白 40 的缺乏与高血压和不规则的动脉血管运动有关。  |  de Wit, C., et al. 2003. Physiol Genomics. 13: 169-77. PMID: 12700362
  5. 心房颤动中的连接蛋白 40 基因(GJA5)体细胞突变。  |  Gollob, MH., et al. 2006. N Engl J Med. 354: 2677-88. PMID: 16790700
  6. 肾素生成细胞中选择性缺失的连接蛋白40会损害肾脏气压感受器的功能,并与动脉高血压有关。  |  Wagner, C., et al. 2010. Kidney Int. 78: 762-8. PMID: 20686449
  7. 缺乏连接蛋白 40 会降低分泌肾素的并肾小球细胞对钙的敏感性。  |  Steppan, D., et al. 2018. Pflugers Arch. 470: 969-978. PMID: 29427253
  8. 连接蛋白40通过ROCK1-MYPT1-MLC20途径调节急性肺损伤中肺内皮的通透性。  |  Yin, J., et al. 2019. Am J Physiol Lung Cell Mol Physiol. 316: L35-L44. PMID: 30234377
  9. 人类心脏间隙连接:分离, 超微结构和蛋白质组成。  |  Manjunath, CK., et al. 1987. J Mol Cell Cardiol. 19: 131-4. PMID: 3573042
  10. 高级糖化终产物通过 AMPK 途径下调糖尿病心房肌细胞中的连接蛋白 43 和连接蛋白 40。  |  Yang, F., et al. 2023. Diabetes Metab Syndr Obes. 16: 3045-3056. PMID: 37810573
  11. 人类膀胱癌细胞中的连接蛋白表达和细胞间通信减少。  |  Grossman, HB., et al. 1994. Cancer Res. 54: 3062-5. PMID: 8187096

订购信息

产品名称产品编号规格价格数量收藏夹

connexin 40 抗体 (B-3)

sc-365107
200 µg/ml
$316.00

connexin 40 (B-3): m-IgG Fc BP-HRP 套装

sc-529311
200 µg Ab; 10 µg BP
$354.00

connexin 40 (B-3): m-IgGκ BP-HRP 套装

sc-522138
200 µg Ab, 40 µg BP
$354.00

connexin 40 抗体 (B-3) AC

sc-365107 AC
500 µg/ml, 25% agarose
$416.00

connexin 40 抗体 (B-3) HRP

sc-365107 HRP
200 µg/ml
$316.00

connexin 40 抗体 (B-3) FITC

sc-365107 FITC
200 µg/ml
$330.00

connexin 40 抗体 (B-3) PE

sc-365107 PE
200 µg/ml
$343.00

connexin 40 抗体 (B-3) Alexa Fluor® 488

sc-365107 AF488
200 µg/ml
$357.00

connexin 40 抗体 (B-3) Alexa Fluor® 546

sc-365107 AF546
200 µg/ml
$357.00

connexin 40 抗体 (B-3) Alexa Fluor® 594

sc-365107 AF594
200 µg/ml
$357.00

connexin 40 抗体 (B-3) Alexa Fluor® 647

sc-365107 AF647
200 µg/ml
$357.00

connexin 40 抗体 (B-3) Alexa Fluor® 680

sc-365107 AF680
200 µg/ml
$357.00

connexin 40 抗体 (B-3) Alexa Fluor® 790

sc-365107 AF790
200 µg/ml
$357.00

I need to visualize Purkinje cells in the heart conductive system of a pig by immunofluorescent. Since this Ab (connexin 40 Antibody (B-3): sc-365107) works for human, could you check the protein sequence for pig

Asked by: Zabihi
Thank you for contacting Santa Cruz Biotechnology. There is a 79% homology for pig. We would not recommend using this antibody for pig samples.
Answered by: BlakeJ
Date published: 2025-05-29

What is the recommended starting dilution for Western blot for sc-365107: connexin 40 (B-3) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from B-3 mAb did not work in IF on heart sectionsI tested this mAb by IF on frozen mouse heart sections, in a dilution range 1:10 to 1:100. Unfortunately the mAb did not work. As a control I unsed an anti Cx40 pAb which gave a good and specific staining.
Date published: 2024-01-12
Rated 5 out of 5 by from 很好用,功能出色很好用,功能很出色,会推荐师弟师妹们使用,我们已经使用这个品牌抗体很久时间了,评价都很好,没有出现过售后
Date published: 2021-08-01
Rated 4 out of 5 by from Buen resultadoFunciona bien a 1:500 en western blot, aunque salen bastantes bandas inespecíficas.
Date published: 2018-02-25
Rated 1 out of 5 by from Immunohistochemistry frozen & free floatingI tested the antibody for an immunohistochemistry on mouse embryo E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/50, Permeabilization solution: PBS 1X- Triton 0,2%(& 0,4%) - Goat serum 10%. It did not work.
Date published: 2017-10-27
Rated 1 out of 5 by from Not functional for parrafin sectionsParaffin sections, Antigen retrieval in Citrate buffer pH:6, blocking in 5% BSA, o/n Ab incubation (1:20). No signal whatsoever.
Date published: 2017-03-22
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of connexin 40 expression in CCD-1064Sk whole cell lysate.
Date published: 2017-02-10
Rated 5 out of 5 by from Good Western Blot data of connexin 40 expressionGood Western Blot data of connexin 40 expression in CCD-1064Sk whole cell lysate. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Great Western Blot data of connexin 40 expressionGreat Western Blot data of connexin 40 expression in JEG-3 whole cell lysate. -SCBT QC
Date published: 2014-11-17
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connexin 40 抗体 (B-3) is rated 3.4 out of 5 by 8.
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