Date published: 2025-9-7
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citrate synthase Antibody (G-3) - Western Blotting - Image 354285
柠檬酸合成酶抗体 (G-3): sc-390693. 柠檬酸合成酶在 Hep G2 (A), HeLa (B), Caco-2 (C) 和 A-673 (D) 全细胞裂解液和小鼠脑组织提取物 (E) 中表达的 Western 印迹分析.
柠檬酸合成酶抗体 (G-3): sc-390693. 柠檬酸合成酶在 Hep G2 (A), HeLa (B), Caco-2 (C) 和 A-673 (D) 全细胞裂解液和小鼠脑组织提取物 (E) 中表达的 Western 印迹分析.

citrate synthase 抗体 (G-3): sc-390693

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说明书
  • citrate synthase抗体(G-3)是小鼠单克隆IgG1 κ, 在40篇文献中引用，规格为200 µg/ml
  • 与human的259-292氨基酸残基的citrate synthase
  • citrate synthase 抗体 (G-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 avian来源的citrate synthase; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗citrate synthase抗体（G-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取citrate synthase (G-3): sc-390693的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 citrate synthase 抗体 (G-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与citrate synthase 抗体 (G-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Citrate synthase 抗体包括 ATP-citrate synthase

柠檬酸合酶抗体（G-3）是一种IgG1 κ小鼠单克隆柠檬酸合酶抗体，它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的柠檬酸合酶。柠檬酸合酶抗体（G-3）既可作为非偶联抗柠檬酸合酶抗体形式，也可作为多种偶联形式的抗柠檬酸合酶抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。柠檬酸合酶（CS）是一种由466个氨基酸组成的线粒体基质蛋白，作为三羧酸循环中的第一个限速酶发挥作用。柠檬酸合酶在线粒体呼吸中必不可少，参与葡萄糖向脂质的转化，在大多数能够进行氧化代谢的细胞中都可以发现。编码柠檬酸合酶的基因定位于人类12号染色体q13.2区，转录成两种可变剪接变体，分别命名为CSa和CSb。人类12号染色体编码超过1100个基因，约占人类基因组的4.5%，并与多种疾病和病症相关，包括软骨生成不全、软骨发育不全、Kniest发育不良、努南综合征和12p三体综合征，后者会导致面部发育缺陷和癫痫发作。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

citrate synthase 抗体 (G-3) 参考文献:

  1. 心肌和骨骼肌耐力训练后柠檬酸合成酶的表达和酶活性。  |  Siu, PM., et al. 2003. J Appl Physiol (1985). 94: 555-60. PMID: 12531911
  2. 人类胰腺癌中柠檬酸合成酶活性增强。  |  Schlichtholz, B., et al. 2005. Pancreas. 30: 99-104. PMID: 15714131
  3. 人类柠檬酸合成酶线粒体靶向序列和机制的鉴定与表征。  |  Cheng, TL., et al. 2009. J Cell Biochem. 107: 1002-15. PMID: 19479947
  4. 人鼠细胞杂化体中 CS, ENO2, GAPDH, LDHB, PEPB 和 TPI 的染色体分配和区域定域。  |  Herbschleb-Voogt, E., et al. 1978. Cytogenet Cell Genet. 22: 482-6. PMID: 318158
  5. 柠檬酸合酶和 OGDH 作为慢性压力下动脉粥样硬化的潜在生物标志物。  |  Meng, LB., et al. 2021. Oxid Med Cell Longev. 2021: 9957908. PMID: 34539976
  6. 来自 Merolae Cyanidioschyzon 的柠檬酸合酶具有很高的草酰乙酸和乙酰-CoA 催化效率。  |  Nishii, M., et al. 2023. Plant Mol Biol. 111: 429-438. PMID: 36884198
  7. 线粒体柠檬酸合酶和柠檬酸在阿尔茨海默病病理生理学中的新作用  |  Chhimpa, N., et al. 2023. J Alzheimers Dis. 94: S453-S472. PMID: 37393492
  8. 普氏假单胞菌柠檬酸合成酶的过表达可提高微氧培养中乙酸产生的琥珀酸。  |  Mutyala, S., et al. 2023. ACS Omega. 8: 26231-26242. PMID: 37521642
  9. 人鼠杂交体细胞线粒体酶的遗传控制。  |  Van Heyningen, V., et al. 1973. Nature. 242: 509-12. PMID: 4633729
  10. 体细胞杂交中柠檬酸合成酶（E.C. 4.1.3.7）的分析程序。  |  Craig, I. 1973. Biochem Genet. 9: 351-8. PMID: 4795824
  11. 在人鼠体细胞杂化体中将柠檬酸合成酶（CS）基因直接分配到人类第 12 号染色体上。  |  Wijnen, LM., et al. 1977. Hum Genet. 39: 339-44. PMID: 598842
  12. 人类柠檬酸合成酶基因的克隆和分子分析。  |  Goldenthal, MJ., et al. 1998. Genome. 41: 733-8. PMID: 9809442

订购信息

产品名称产品编号规格价格数量收藏夹

citrate synthase 抗体 (G-3)

sc-390693
200 µg/ml
$316.00

citrate synthase (G-3): m-IgG Fc BP-HRP 套装

sc-530230
200 µg Ab; 10 µg BP
$354.00

citrate synthase (G-3): m-IgGκ BP-HRP 套装

sc-523640
200 µg Ab, 40 µg BP
$354.00

citrate synthase 抗体 (G-3) AC

sc-390693 AC
500 µg/ml, 25% agarose
$416.00

citrate synthase 抗体 (G-3) HRP

sc-390693 HRP
200 µg/ml
$316.00

citrate synthase 抗体 (G-3) FITC

sc-390693 FITC
200 µg/ml
$330.00

citrate synthase 抗体 (G-3) PE

sc-390693 PE
200 µg/ml
$343.00

citrate synthase 抗体 (G-3) Alexa Fluor® 488

sc-390693 AF488
200 µg/ml
$357.00

citrate synthase 抗体 (G-3) Alexa Fluor® 546

sc-390693 AF546
200 µg/ml
$357.00

citrate synthase 抗体 (G-3) Alexa Fluor® 594

sc-390693 AF594
200 µg/ml
$357.00

citrate synthase 抗体 (G-3) Alexa Fluor® 647

sc-390693 AF647
200 µg/ml
$357.00

citrate synthase 抗体 (G-3) Alexa Fluor® 680

sc-390693 AF680
200 µg/ml
$357.00

citrate synthase 抗体 (G-3) Alexa Fluor® 790

sc-390693 AF790
200 µg/ml
$357.00

citrate synthase (G-3) 中和勝肽

sc-390693 P
100 µg/0.5 ml
$68.00

Does the antibody have reactivity to Drosophila citrate synthase? Is the used antigen region conserved?

Asked by: Hirosh
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-18

What do you recommend for secondary detection in a Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-13

I have a double stain planned. How can I do this using only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-390693, is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 10
Date published: 2017-01-13
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Rated 5 out of 5 by from Great antibody for IF in mouse and human cellsExtremely good signal for IF. I use it at 1:500 dilution. The mitochondrial networks look great in mouse and human cells. I highly recommend this AB for IF though most people stick to TOM proteins, this is a great option and looks better than the TOM proteins.
Date published: 2019-09-06
Rated 4 out of 5 by from Good antibodyI used it in mice iWAT. It worked very well. I got strong and clean bands
Date published: 2019-03-01
Rated 5 out of 5 by from excellent antibodyUsed in wb in N2A cell lysates, in dilution 1:1000. Very strong bands, without backround.
Date published: 2018-05-11
Rated 4 out of 5 by from Works fine for WBI used 50µg of isolated mitochondria from mouse hearts separated on a 10% SDS-gel. One prominent band of app. 40k Da and one weak band at 25 kDa can be observed. Can be used as a loading control for mitochondrial preparations.
Date published: 2018-04-05
Rated 5 out of 5 by from the results are goodI am so sorry to give the feedback now. I got the sample about 2 months ago, but the results are satisfactory。My test is about C2C12 cells, your sample was used in western blot, the ratio is 1:1000.
Date published: 2017-10-23
Rated 5 out of 5 by from Wow !! OutstandingSuperb western blot, IF and IHC staining data; great product
Date published: 2017-01-23
Rated 5 out of 5 by from Great for WBThis citrate synthase antibody shows good results in WB for human and mouse samples.
Date published: 2017-01-14
Rated 5 out of 5 by from Good data used in wbThis antibody received two weeks ago,and I had good data for CS in Hela A549 cell.
Date published: 2016-11-29
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citrate synthase 抗体 (G-3) is rated 4.8 out of 5 by 12.
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