Date published: 2025-9-8
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CHIP/STUB1 Antibody (G-2) - Western Blotting - Image 30328
CHIP 抗体 (G-2): sc-133066. HeLa (A), HEK293 (B), MIA PACA-2 (C) 和 A-10 (D) 全细胞裂解液中 CHIP 表达的 Western 印迹分析.
CHIP/STUB1 Antibody (G-2) - Western Blotting - Image 30328
CHIP 抗体 (G-2): sc-133066. HeLa (A), HEK293 (B), MIA PACA-2 (C) 和 A-10 (D) 全细胞裂解液中 CHIP 表达的 Western 印迹分析.
CHIP/STUB1 Antibody (G-2) - Western Blotting - Image 45051
CHIP 抗体 (G-2): sc-133066. 未转染: sc-117752 (A) 和小鼠 CHIP 转染: sc-119227 (B) 293T 全细胞裂解液中 CHIP 表达的 Western 印迹分析.
CHIP/STUB1 Antibody (G-2) - Western Blotting - Image 59363
CHIP 抗体 (G-2): sc-133066. 未转染 293T: sc-117752 (A), 转染人 CHIP 的 293T: sc-170019 (B) 和 HeLa (C) 全细胞裂解液中 CHIP 表达的 Western 印迹分析.
CHIP 抗体 (G-2): sc-133066. HeLa (A), HEK293 (B), MIA PACA-2 (C) 和 A-10 (D) 全细胞裂解液中 CHIP 表达的 Western 印迹分析.

CHIP/STUB1 抗体 (G-2): sc-133066

4.6(11)
寫評論提問

说明书
  • CHIP/STUB1 抗体 G-2 是小鼠单克隆 IgG1 κ，CHIP/STUB1抗体, 在35篇文献中引用，规格为200 µg/ml
  • 针对氨基酸73-303，在CHIP的human起源的C-terminus处进行映射
  • CHIP/STUB1 抗体 (G-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CHIP
  • 抗CHIP/STUB1抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CHIP/STUB1 (G-2): sc-133066的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CHIP/STUB1 Antibody (G-2) for WB applications. 的首选辅助检测试剂，这些试剂现与CHIP/STUB1 Antibody (G-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CHIP抗体（G-2）是一种IgG1κ小鼠单克隆CHIP抗体（也称为STUB1抗体或E3泛素蛋白连接酶CHIP抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源CHIP蛋白。CHIP抗体（G-2）既可作为非偶联抗CHIP抗体形式，也可作为多种偶联形式的抗CHIP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CHIP（HSP70相互作用蛋白的羧基末端），也称为STIP1同源和含U-box的蛋白1、HSPABP2、NY-CO-7、SDCCAG7和STUB1，是一种影响蛋白质泛素化的细胞质E3泛素连接酶。CHIP与Smad1/Smad4相互作用，并通过泛素介导的Smad蛋白降解阻断BMP信号传导。CHIP控制Hsp70/Hsp90伴侣与ErbB2的结合以及Hsp90抑制剂诱导的ErbB2下调。一条1.3kb的转录本在横纹肌（心脏和骨骼肌）中含量最高，在胰腺和脑中的表达较低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CHIP/STUB1 抗体 (G-2) 参考文献:

  1. 鉴定 CHIP，它是一种新型含四肽重复蛋白，能与热休克蛋白相互作用并负向调节伴侣功能。  |  Ballinger, CA., et al. 1999. Mol Cell Biol. 19: 4535-45. PMID: 10330192
  2. CHIP 是一种依赖于 U-box 的 E3 泛素连接酶：确定 Hsc70 为泛素化的靶标。  |  Jiang, J., et al. 2001. J Biol Chem. 276: 42938-44. PMID: 11557750
  3. CHIP 与 Parkin（一种导致家族性帕金森病的基因）缔合作用，可增强其泛素连接酶的活性。  |  Imai, Y., et al. 2002. Mol Cell. 10: 55-67. PMID: 12150907
  4. 依赖于伴侣的E3泛素连接酶CHIP介导了c-ErbB2/Neu的降解途径。  |  Xu, W., et al. 2002. Proc Natl Acad Sci U S A. 99: 12847-52. PMID: 12239347
  5. 辅助伴侣/泛素连接酶 CHIP 对内皮一氧化氮合成酶胞内运输的伴侣依赖性调控。  |  Jiang, J., et al. 2003. J Biol Chem. 278: 49332-41. PMID: 14507928
  6. 辅助伴侣 HspBP1 可抑制 CHIP 泛素连接酶并刺激囊性纤维化跨膜传导调节因子的成熟。  |  Alberti, S., et al. 2004. Mol Biol Cell. 15: 4003-10. PMID: 15215316
  7. 鸟氨酸脱羧酶及其调节蛋白抗酶-1的细胞内定位。  |  Schipper, RG., et al. 2004. J Histochem Cytochem. 52: 1259-66. PMID: 15385572
  8. 抑制 Hsc70-CHIP E3 泛素连接酶后，一种可折叠的 CFTR{Delta}F508 生物中间体会累积。  |  Younger, JM., et al. 2004. J Cell Biol. 167: 1075-85. PMID: 15611333

订购信息

产品名称产品编号规格价格数量收藏夹

CHIP/STUB1 抗体 (G-2)

sc-133066
200 µg/ml
$316.00

CHIP/STUB1 (G-2): m-IgG Fc BP-HRP 套装

sc-527090
200 µg Ab; 10 µg BP
$354.00

CHIP/STUB1 (G-2): m-IgG1 BP-HRP 套装

sc-532463
200 µg Ab; 20 µg BP
$354.00

CHIP/STUB1 抗体 (G-2) AC

sc-133066 AC
500 µg/ml, 25% agarose
$416.00

CHIP/STUB1 抗体 (G-2) HRP

sc-133066 HRP
200 µg/ml
$316.00

CHIP/STUB1 抗体 (G-2) FITC

sc-133066 FITC
200 µg/ml
$330.00

CHIP/STUB1 抗体 (G-2) PE

sc-133066 PE
200 µg/ml
$343.00

CHIP/STUB1 抗体 (G-2) Alexa Fluor® 488

sc-133066 AF488
200 µg/ml
$357.00

CHIP/STUB1 抗体 (G-2) Alexa Fluor® 546

sc-133066 AF546
200 µg/ml
$357.00

CHIP/STUB1 抗体 (G-2) Alexa Fluor® 594

sc-133066 AF594
200 µg/ml
$357.00

CHIP/STUB1 抗体 (G-2) Alexa Fluor® 647

sc-133066 AF647
200 µg/ml
$357.00

CHIP/STUB1 抗体 (G-2) Alexa Fluor® 680

sc-133066 AF680
200 µg/ml
$357.00

CHIP/STUB1 抗体 (G-2) Alexa Fluor® 790

sc-133066 AF790
200 µg/ml
$357.00

This antibody, CHIP (G-2): sc-133066, is specifically cited in the article (PMID: # 24651434 ) for use in Immunohistochemistry. Why didn't you put IHC under the Application of the manual?

Asked by: songxj12
Thank you for your question. For our applications we will not recommend a product for IHC(P) unless it is validated in house or is well established for this application in multiple publications. We will however cover the antibody by warranty for this application based on the publication record.
Answered by: Technical Service
Date published: 2017-04-19

Are there any issues with background when using this antibody to stain mouse tissue?

Asked by: AbPolly
Thank you for your question. This antibody is recommended for use with mouse tissue. In order to avoid any potential background issues with anti-mouse secondaries, we recommend using a directly conjugated version. This antibody is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 1
Date published: 2017-01-16

You said this antibody was used in the article (PMID: # 24651434. ), but according to the article the anti-CHIP antibody used in it was from Cell Signaling (Boston, MA, USA) .

Asked by: songxj12
Thank you for your question. This antibody, CHIP (G-2): sc-133066, is specifically cited in this publication under the Materials and Methods for use in Immunohistochemistry. This is separate from the "Antibodies" section of Materials and Methods. See an excerpt from the publication below: "The primary antibody incubation was completed at 4°C overnight in a humidified chamber with the following antibodies: mouse monoclonal antibody PI3K-p85 (sc-1673, Santa Cruz Biotechnology; dilution 1 : 150), mouse monoclonal antibody CHIP (sc-133066, Santa Cruz Biotechnology; dilution 1 : 200)..."
Answered by: Technical Support
Date published: 2017-04-19
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Rated 2 out of 5 by from Ok for WB, very poor for IP-No pull down of CHIP, no differences in output vs input and barely any signal in FT
Date published: 2019-04-30
Rated 5 out of 5 by from it is usefull!in lm3 cell,it is usefull and about 35kd diluted 1:500
Date published: 2017-08-07
Rated 5 out of 5 by from Strong and clean in WBUsed at 1:200 for WB OF MCF7. Performed very well.
Date published: 2017-06-26
Rated 4 out of 5 by from Works for WBFaint but distinct bands for WB. Used the antibody at 1:1000
Date published: 2017-06-07
Rated 5 out of 5 by from I used this antibody for WBThe protein used for the WB is extraced from H9C2 cell,the result is clear and special!
Date published: 2017-02-15
Rated 5 out of 5 by from Nice Westernblot bandI got nice single Bands with this Antibody at the predicted size using fibroblast whole cell lysate (RIPA). the antibody was used 1:1000 and secondary AB was used 1:5000.
Date published: 2017-01-30
Rated 5 out of 5 by from Works great for WBCHIP (G-2) gives a strong band at 35 kDa in HeLa whole cell lysate.
Date published: 2016-12-22
Rated 5 out of 5 by from I used this antibody successfully in WesternI used this antibody successfully in Western for my publication.
Date published: 2015-09-10
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CHIP/STUB1 抗体 (G-2) is rated 4.6 out of 5 by 11.
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