Date published: 2025-9-6
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CUTL1/CUX1/CDP Antibody (B-10) - Western Blotting - Image 314151
CDP 抗体 (B-10): sc-514008. Western 印迹分析 BJAB (A) 和 K-562 (B) 核提取物中 CDP 的表达.
CDP 抗体 (B-10): sc-514008. Western 印迹分析 BJAB (A) 和 K-562 (B) 核提取物中 CDP 的表达.

CUTL1/CUX1/CDP 抗体 (B-10): sc-514008

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说明书
  • CUTL1/CUX1/CDP 抗体 B-10 是小鼠单克隆 IgG3 κ，CUTL1/CUX1/CDP抗体, 在22篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的CDP的C-terminus的氨基酸1308-1332之间
  • 推荐用于 mouse, rat 和 human 来源的CDP WB, IP, IF, IHC(P) 和 ELISA检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 可用于ChIP的TransCruz试剂(sc-514008 X, 200 µg/0.1 ml)
  • 关于如何获取CUTL1/CUX1/CDP (B-10): sc-514008的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP 是CUTL1/CUX1/CDP 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与CUTL1/CUX1/CDP 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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研究信息

関連項目

CDP抗体（B-10）是一种IgG3 κ小鼠单克隆CDP抗体（也称为CUX1抗体或CUTL1抗体），可以通过WB、IP、IF、IHC(P)和ELISA检测到来自小鼠、大鼠和人类的CDP蛋白。CDP抗体（B-10）既有非偶联形式的抗CDP抗体，也有多种偶联形式的抗CDP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CDP（CCAAT置换蛋白）已被确定为发育调控基因转录的阻遏物。它是一种同源结构域蛋白，似乎与转录激活蛋白竞争结合各种基因的启动子区域。CDP包含三个作为DNA结合结构域的剪切重复序列。已经证明，剪切重复序列结构域具有与同源结构域DNA结合协同或独立地结合DNA的能力。CDP已被证明是HiNF-D复合物的DNA结合亚基，该复合物除了CDP外，还含有细胞周期蛋白A、Cdc2和一种Rb相关蛋白。组蛋白表达是细胞周期向S期过渡所必需的。HiNF-D复合物调节组蛋白H4、H3和H1基因的转录，使细胞从G1期进展到S期。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CUTL1/CUX1/CDP 抗体 (B-10) 参考文献:

  1. 人类 CCAAT 位移蛋白与果蝇同源蛋白 cut 同源。  |  Neufeld, EJ., et al. 1992. Nat Genet. 1: 50-5. PMID: 1301999
  2. DREF 和 Cut 对果蝇 PCNA 基因启动子的颉颃物调控。  |  Seto, H., et al. 2006. Genes Cells. 11: 499-512. PMID: 16629902
  3. 分化诱导的Cutl1/CDP裂解产生一种新型显性阴性异构体，可调控乳腺基因的表达。  |  Maitra, U., et al. 2006. Mol Cell Biol. 26: 7466-78. PMID: 17015474
  4. 表达 p75 CCAAT 置换蛋白/Cut 同源异构体的转基因小鼠会患上类似骨髓增生性疾病的骨髓性白血病。  |  Cadieux, C., et al. 2006. Cancer Res. 66: 9492-501. PMID: 17018605
  5. 人类切割同源结构域蛋白中的保守切割重复序列具有 DNA 结合域的功能。  |  Harada, R., et al. 1994. J Biol Chem. 269: 2062-7. PMID: 7904999
  6. 小鼠同源结构域蛋白 Phox2 与 Cux/CDP 共同调节 Ncam 启动子的活性，可能是神经递质表型的决定因素。  |  Valarché, I., et al. 1993. Development. 119: 881-96. PMID: 7910552
  7. CCAAT 位移蛋白与多种转录激活因子竞争结合到 gp91phox 启动子近端四个位点上。  |  Luo, W. and Skalnik, DG. 1996. J Biol Chem. 271: 18203-10. PMID: 8663528
  8. CDP/cut 是组蛋白基因转录因子 HiNF-D 的 DNA 结合亚基：独立于转录因子 E2F 的 G1/S 期细胞周期转换点基因调控机制。  |  van Wijnen, AJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 11516-21. PMID: 8876167

订购信息

产品名称产品编号规格价格数量收藏夹

CUTL1/CUX1/CDP 抗体 (B-10)

sc-514008
200 µg/ml
$316.00

CUTL1/CUX1/CDP (B-10): m-IgG3 BP-HRP 套装

sc-550607
200 µg Ab; 40 µg BP
$354.00

CUTL1/CUX1/CDP 抗体 (B-10) AC

sc-514008 AC
500 µg/ml, 25% agarose
$416.00

CUTL1/CUX1/CDP 抗体 (B-10) Alexa Fluor® 488

sc-514008 AF488
200 µg/ml
$357.00

CUTL1/CUX1/CDP 抗体 (B-10) Alexa Fluor® 647

sc-514008 AF647
200 µg/ml
$357.00

CUTL1/CUX1/CDP 抗体 (B-10) FITC

sc-514008 FITC
200 µg/ml
$330.00

CUTL1/CUX1/CDP 抗体 (B-10) HRP

sc-514008 HRP
200 µg/ml
$316.00

CUTL1/CUX1/CDP (B-10) 中和勝肽

sc-514008 P
100 µg/0.5 ml
$68.00

CUTL1/CUX1/CDP 抗体 (B-10) PE

sc-514008 PE
200 µg/ml
$343.00

CUTL1/CUX1/CDP 抗体 (B-10) X

sc-514008 X
200 µg/0.1 ml
$316.00

Hi, I used this antibody for my whole mount brain imaging with the technique called iDISCO, and I saw good staining. I saw the signal of Cux1 is mainly in the cytosol instead of the nucleus, which is suggested by the literatures. 

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-11

Hello, I am interested in ordering this antibody for IP. I have some literature citing old polyclonal Ab for IP with great success but the reviews on this monoclonal are worrisome. Also, I am interested in using magnetic beads for labeling. Please advice.

Asked by: sbagla
Thank you for your question. This antibody has been established for IP applications in samples of mouse, rat and human origin in our labs and offer our warranty for this use. If you purchase the antibody and it does not perform as expected in the recommended species and application, please contact our Technical Service Department. If we are unable to help you troubleshoot the antibody, we can provide you with a complimentary replacement antibody, credit or refund. Our warranty is for one year from the date of purchase.
Answered by: Tech Support Europe
Date published: 2019-06-12

Hi there! I've been looking at the wonderful IHC images obtained with the CDP M-222 antibody; however, I'm concerned with the bad reviews of its replacement. I want to use CDP B-10 for PFA fixed free floating mouse brain sections.

Asked by: JuanSA
Thank you for your question. We have not tested this antibody in free floating mouse brain tissue sections. However, we have had excellent results using the antibody in formalin-fixed paraffin-embedded human thyroid and upper stomach tissues. Unlike in some of the customer product reviews, we used the primary antibody at a dilution of 1:50. We do warranty the antibody to work for mouse tissues. If you purchase the antibody and it does not perform as expected in the recommended species and application, please contact our Technical Service Department. If we are unable to help you troubleshoot the antibody, we can provide you with a complimentary replacement antibody, credit or refund. Our warranty is for one year from the date of purchase.
Answered by: Technical Support
Date published: 2018-11-21

What application is the blocking peptide sc-514008 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 5 out of 5 by from Good antibodyThe antibody arrived quickly. It works well for immunostaining
Date published: 2025-07-07
Rated 1 out of 5 by from No signal at allWe tested this antibody on mouse embryonic brain tissue to stain neocortical upper layers. No signal at all with or without antigen retrieval. It's a shame that M222 antibody was replaced by this one.
Date published: 2020-03-04
Rated 1 out of 5 by from Didn't WorkOur lab has previously used and gotten great results from the SCBT's discontinued polyclonal CDP ab. We purchased the monoclonal since the poly was no longer available, but it did not work. Despite multiple attempts with and without antigen retrieval from two different lot numbers of the antibody, we got no results. SCBT has, however, been extremely helpful in terms of providing a second aliquot of no charge. They responded quickly and we are very happy with their customer service.
Date published: 2019-04-16
Rated 1 out of 5 by from Did not work for mouse brain IHCWe tested this antibody in free floating sections of perfused mouse brain. No specific signal was observed at a 1:100 dilution. Antigen retrieval with citrate buffer pH 6.0 at 85°C for 25 min also failed to produce any specific staining.
Date published: 2018-11-15
Rated 1 out of 5 by from No bands were observed in WBUsed 1:100 dilution for mouse tissue (Liver, brain) as well as AML12 hepatocyte cell line nuclear and cytoplasmic proteins for 1 overnight incubation in 5% milk TBS-T blocking buffer. No bands whatsoever was observed. Actin and Nucleolin band as loading control was very good, so it was not the protein problem. As well, same secondary Ab was used for the Actin that was used with this Ab, so it was not the secondary antibody issue either.
Date published: 2018-03-20
Rated 1 out of 5 by from That's too bad..I got the sample of this and tried. No matter how I tried to stain with this antibody, there's only very blurred signal on the sections
Date published: 2018-02-26
Rated 1 out of 5 by from Did not work for IFTried it for IF on mouse brain slices (1:500) and it did not work
Date published: 2018-01-30
Rated 5 out of 5 by from niceGreat antibody！1：1000 used in the u251 cells by Western Blotting, I got satisfactory results.
Date published: 2017-11-03
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CUTL1/CUX1/CDP 抗体 (B-10) is rated 2.7 out of 5 by 19.
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