Date published: 2025-9-7
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CDKL5 Antibody (D-12) - Western Blotting - Image 366900
CDKL5 抗体 (D-12): sc-376314. A549 (A) 和 Neuro-2A (B) 全细胞裂解液中 CDKL5 表达的 Western 印迹分析.
CDKL5 抗体 (D-12): sc-376314. A549 (A) 和 Neuro-2A (B) 全细胞裂解液中 CDKL5 表达的 Western 印迹分析.

CDKL5 抗体 (D-12): sc-376314

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说明书
  • CDKL5 抗体 D-12 是小鼠单克隆 IgG2b κ，CDKL5抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列222-520位于human物种的CDKL5的内部区域内
  • CDKL5 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CDKL5
  • 抗CDKL5抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CDKL5 (D-12): sc-376314的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是CDKL5 Antibody (D-12) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与CDKL5 Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CDKL5抗体（D-12）是一种IgG2b κ小鼠单克隆CDKL5抗体（也称为CDKL5抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的CDKL5蛋白。CDKL5抗体（D-12）既有非偶联的抗CDKL5抗体形式，也有多种偶联形式的抗CDKL5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞周期的进展部分由一组细胞周期蛋白和细胞周期蛋白依赖性激酶（Cdks）控制。Cdk蛋白与细胞周期蛋白协同作用，磷酸化细胞周期进展每个阶段的关键底物。另一类蛋白，即Cdk抑制剂，也通过与细胞周期蛋白-Cdk复合物结合并调节其活性，在细胞周期调控中发挥作用。CDKL5（细胞周期蛋白依赖性激酶样5）是一种含有1030个氨基酸的蛋白质，属于CMGC Ser/Thr蛋白激酶家族。CDKL5在脑、肺、肾、前列腺、卵巢、胎盘、胰腺和睾丸中表达，被认为在细胞周期调控中发挥作用。CDKL5的缺陷是多种疾病的原因，如X连锁婴儿痉挛综合征和雷特综合征。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CDKL5 抗体 (D-12) 参考文献:

  1. X 连锁细胞周期蛋白依赖性激酶样 5（CDKL5/STK9）基因突变与严重的神经发育迟缓有缔合作用。  |  Tao, J., et al. 2004. Am J Hum Genet. 75: 1149-54. PMID: 15499549
  2. CDKL5 基因突变导致的肌阵挛性脑病。  |  Buoni, S., et al. 2006. Clin Neurophysiol. 117: 223-7. PMID: 16326141
  3. CDKL5 基因突变会导致女性患者出现婴儿痉挛, 早发癫痫和严重智力障碍。  |  Archer, HL., et al. 2006. J Med Genet. 43: 729-34. PMID: 16611748
  4. CDKL5新型C端截断突变的母源导致严重的非典型Rett综合征。  |  Nectoux, J., et al. 2006. Clin Genet. 70: 29-33. PMID: 16813600
  5. CDKL5基因突变的功能性后果，CDKL5是一种与婴儿痉挛和智力迟钝有关的X连锁基因。  |  Bertani, I., et al. 2006. J Biol Chem. 281: 32048-56. PMID: 16935860
  6. CDKL5 突变中的癫痫发作和脑电图发现：病例报告和综述。  |  Grosso, S., et al. 2007. Brain Dev. 29: 239-42. PMID: 17049193
  7. 一名患有 CDKL5 和 NHS 基因 Xp22 间质缺失的男孩出现脑病和双侧白内障。  |  Van Esch, H., et al. 2007. Am J Med Genet A. 143: 364-9. PMID: 17256798
  8. CDKL5 基因突变患者癫痫的三个阶段。  |  Bahi-Buisson, N., et al. 2008. Epilepsia. 49: 1027-37. PMID: 18266744
  9. CDKL5缺乏症相关癫痫：当前和新兴治疗综述。  |  Hong, W., et al. 2022. CNS Drugs. 36: 591-604. PMID: 35633486
  10. CDKL5缺乏症：分子见解和致病机制，有助于加快治疗研发进程。  |  Van Bergen, NJ., et al. 2022. Biochem Soc Trans. 50: 1207-1224. PMID: 35997111
  11. 成人谷氨酸能神经元中的CDKL5缺乏会改变突触活性，并通过TrkB信号传导导致自发癫痫发作。  |  Zhu, ZA., et al. 2023. Cell Rep. 42: 113202. PMID: 37777961
  12. CDKL5调节p62介导的选择性自噬，并具有抗神经病毒作用。  |  Thinwa, JW., et al. 2024. J Clin Invest. 134: PMID: 37917202

订购信息

产品名称产品编号规格价格数量收藏夹

CDKL5 抗体 (D-12)

sc-376314
200 µg/ml
$316.00

CDKL5 (D-12): m-IgG2b BP-HRP 套装

sc-548755
200 µg Ab; 10 µg BP
$354.00

CDKL5 抗体 (D-12) AC

sc-376314 AC
500 µg/ml, 25% agarose
$416.00

CDKL5 抗体 (D-12) HRP

sc-376314 HRP
200 µg/ml
$316.00

CDKL5 抗体 (D-12) FITC

sc-376314 FITC
200 µg/ml
$330.00

CDKL5 抗体 (D-12) PE

sc-376314 PE
200 µg/ml
$343.00

CDKL5 抗体 (D-12) Alexa Fluor® 488

sc-376314 AF488
200 µg/ml
$357.00

CDKL5 抗体 (D-12) Alexa Fluor® 546

sc-376314 AF546
200 µg/ml
$357.00

CDKL5 抗体 (D-12) Alexa Fluor® 594

sc-376314 AF594
200 µg/ml
$357.00

CDKL5 抗体 (D-12) Alexa Fluor® 647

sc-376314 AF647
200 µg/ml
$357.00

CDKL5 抗体 (D-12) Alexa Fluor® 680

sc-376314 AF680
200 µg/ml
$357.00

CDKL5 抗体 (D-12) Alexa Fluor® 790

sc-376314 AF790
200 µg/ml
$357.00

I am using this antibody for WB for WT and KO mice however it is showing bands in both lanes (for WT and KO) at the expected MW of the protein. Has this problem occured before? Behavioural tests on these mice do show that they are KO.

Asked by: Erva Ghani
Thank you for your question. Please contact our technical service team for troubleshooting advice.
Answered by: Tech Support Europe
Date published: 2023-07-24

HELLO, I am using this antibody for WB, but i am getting lots of unspecific binding, the dilution I am doing is 1:2500, and for the blocking I am using milk. any suggestions or recommendations to get better results?

Asked by: Marie256
Thank you for your question. For western blotting experiments with this antibody we recommend a starting dilution of 1:100, and a dilution range between 1:100-1:1000. If the issue persists with dilutions within the recommended range please contact our technical service team for in-depth troubleshooting of your experiment.
Answered by: Tech Support Europe
Date published: 2022-01-11

Has this antibody been tested in IF on WT and Knock Out cells or in cells where CDKL5 has been knocked down? Because in my hands, it seems to give good nuclear signal even in cells where CDKL5 has been knocked out using CRISPR or knocked down using siRNA.

Asked by: affu
Contact Technical Service for more information.
Answered by: Tech Sales
Date published: 2021-07-09

What is the recommended starting dilution for Western blot for CDKL5 (D-12): sc-376314 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-376314.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 14
Date published: 2017-01-20
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Rated 5 out of 5 by from SuperbNice western blot data; strong, clear signal; also great IF staining
Date published: 2017-02-04
Rated 5 out of 5 by from strong signalThis antibody is a sensitive and strong antibody for CDKL5 detection. Very good for WB!
Date published: 2016-12-21
Rated 5 out of 5 by from Superb immunofluorescence nuclear stainingSuperb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-23
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CDKL5 抗体 (D-12) is rated 5.0 out of 5 by 4.
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