Cdc7 Antibody (SPM171): sc-56275

Cdc7 Antibody (SPM171) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Cdc7 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). anti-Cdc7 antibody (SPM171) is available as a non-conjugated format. Cdc7 protein plays a crucial role in the regulation of DNA replication, specifically by activating replication origins during the S phase of the cell cycle. Cdc7 forms a vital complex with the regulatory subunit Dbf4, which is essential for kinase activity. This complex is responsible for the phosphorylation of several key proteins necessary for initiating DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. Precise regulation of Cdc7 and Dbf4 is critical, as Dbf4 levels fluctuate throughout the cell cycle, being absent during the G1 phase and accumulating during S and G2 phases. Timely degradation of Dbf4 by the anaphase-promoting complex at the onset of chromosome segregation ensures that prereplicative complexes do not refire immediately, thereby maintaining genomic stability. Consistent expression of Cdc7 throughout the cell cycle underscores its importance in DNA replication and cell division, making Cdc7 (SPM171) antibody an invaluable tool for researchers studying cell cycle regulation and related processes.

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Cdc7 Antibody (SPM171) References:

1. A role for the Cdc7 kinase regulatory subunit Dbf4p in the formation of initiation-competent origins of replication.  |  Pasero, P., et al. 1999. Genes Dev. 13: 2159-76. PMID: 10465792
2. Cdc7p-Dbf4p kinase binds to chromatin during S phase and is regulated by both the APC and the RAD53 checkpoint pathway.  |  Weinreich, M. and Stillman, B. 1999. EMBO J. 18: 5334-46. PMID: 10508166
3. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes.  |  Lepke, M., et al. 1999. Mol Gen Genet. 262: 220-9. PMID: 10517317
4. Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication.  |  Jiang, W., et al. 1999. EMBO J. 18: 5703-13. PMID: 10523313
5. Dbf4p, an essential S phase-promoting factor, is targeted for degradation by the anaphase-promoting complex.  |  Ferreira, MF., et al. 2000. Mol Cell Biol. 20: 242-8. PMID: 10594027
6. Cdc7 kinase complex: a key regulator in the initiation of DNA replication.  |  Masai, H. and Arai, K. 2002. J Cell Physiol. 190: 287-96. PMID: 11857444
7. Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin.  |  Masai, H., et al. 2006. J Biol Chem. 281: 39249-61. PMID: 17046832
8. Cdc7 is an active kinase in human cancer cells undergoing replication stress.  |  Tenca, P., et al. 2007. J Biol Chem. 282: 208-15. PMID: 17062569
9. CDC7-independent G1/S transition revealed by targeted protein degradation.  |  Suski, JM., et al. 2022. Nature. 605: 357-365. PMID: 35508654
10. CDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockade.  |  Morita, TY., et al. 2023. Nat Commun. 14: 7490. PMID: 37980406
11. CDC7 inhibition impairs neuroendocrine transformation in lung and prostate tumors through MYC degradation.  |  Quintanal-Villalonga, A., et al. 2024. Signal Transduct Target Ther. 9: 189. PMID: 39054323
12. The Cdc7 protein kinase is required for origin firing during S phase.  |  Bousset, K. and Diffley, JF. 1998. Genes Dev. 12: 480-90. PMID: 9472017

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc7 Antibody (SPM171) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc7 Antibody (SPM171) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc7 Antibody (SPM171) Specifications

Alternate Product Name: Cdc7

Product Clone Name: SPM171

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cdc7

Epitope Species: human

Product Reactivity/Detection: Cdc7

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Cdc7 Antibody (SPM171): N/A