Date published: 2026-10-22
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Cdc25A Antibody (DCS-120) - Western Blotting - Image 352423
Cdc25A 抗体 (DCS-120): sc-56264. K-562 (A), SK-N-MC (B), BJAB (C) 和 MCF7 (D) 全细胞裂解液中 Cdc25A 表达的 Western 印迹分析.
Cdc25A 抗体 (DCS-120): sc-56264. K-562 (A), SK-N-MC (B), BJAB (C) 和 MCF7 (D) 全细胞裂解液中 Cdc25A 表达的 Western 印迹分析.

Cdc25A 抗体 (DCS-120): sc-56264

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寫評論提問
说明书
  • Cdc25A抗体(DCS-120)是小鼠单克隆IgG2a κ, 在9篇文献中引用，规格为200 µg/ml
  • 针对纯化的重组Cdc25A
  • 推荐用于 mouse, rat 和 human 来源的Cdc25A WB, IP, IF 和 IHC(P)检测
  • 看 Cdc25A (F-6): sc-7389，可查看有标记的 Cdc25A 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Cdc25A 抗体 (DCS-120)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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研究信息

関連項目

附加Cdc抗体，包括Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26, Cdc27, Cdc28, Cdc34, Cdc37, Cdc4, Cdc40和Cdc42

参与真核细胞有丝分裂调控的Cdc2/cyclin B酶受到多个层次的调控。其中，通过酪氨酸磷酸化对催化亚基的调控是最为了解的。酪氨酸磷酸化抑制Cdc2/cyclin B复合物，而酪氨酸去磷酸化在有丝分裂开始时发生，直接激活前MPH复合物。Cdc25基因作为限速的有丝分裂激活剂，显然是由于其作为Cdc2酪氨酸磷酸酶的作用。在缺乏Cdc25的情况下，Cdc2会累积在酪氨酸磷酸化的状态下。此外，来自多种物种的Cdc25蛋白已被证明与其他酪氨酸磷酸酶具有较低程度的序列相似性。Cdc25基因家族至少包含三个成员，在其最保守的羧基端序列中共享约40%的同源性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc25A 抗体 (DCS-120) 参考文献:

  1. Cdc25A蛋白磷酸酶：肝癌治疗的一个治疗靶点。  |  Wang, Z., et al. 2008. Anticancer Agents Med Chem. 8: 863-71. PMID: 19075569
  2. CyclinD-CDK4/6 复合物使 CDC25A 磷酸化，并调节其稳定性。  |  Dozier, C., et al. 2017. Oncogene. 36: 3781-3788. PMID: 28192398
  3. 磷酸酶 Cdc25A 通过抑制 TBK1 的活性消极调节抗病毒免疫反应  |  Qi, D., et al. 2018. J Virol. 92: PMID: 30021902
  4. HAUSP 可稳定 Cdc25A，保护宫颈癌细胞免受 DNA 损伤反应的影响。  |  Das, S., et al. 2020. Biochim Biophys Acta Mol Cell Res. 1867: 118835. PMID: 32860838
  5. CDC25A 磷酸酶的激活受到 CDK2/cyclin A 介导的反馈抑制的限制。  |  Ditano, JP., et al. 2021. Cell Cycle. 20: 1308-1319. PMID: 34156324
  6. Hsa_circ_0014879通过miR-519-3p/CDC25A轴调节食管鳞状细胞癌的放射敏感性。  |  Liu, Z., et al. 2022. Anticancer Drugs. 33: e349-e361. PMID: 34407051
  7. miR-100-5p和CDC25A在乳腺癌细胞中的作用。  |  Li, X., et al. 2022. PeerJ. 9: e12263. PMID: 35036112
  8. 小分子Cdc25A抑制剂可保护神经细胞免受NGF剥夺和6-羟基多巴胺引起的死亡。  |  Pramanik, SK., et al. 2023. ACS Chem Neurosci. 14: 1226-1237. PMID: 36942687
  9. 一种基于HDACi的新型硒类似物通过CDC25A诱导细胞凋亡和细胞周期停滞，从而改善前列腺癌的治疗。  |  Shi, Z., et al. 2024. Theranostics. 14: 3565-3582. PMID: 38948069
  10. m6A修饰增强CDC25A的稳定性，通过细胞周期促进食管胃连接部腺癌的肿瘤发生。  |  Pan, Y., et al. 2024. Int J Biol Sci. 20: 4209-4221. PMID: 39247830

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc25A 抗体 (DCS-120)

sc-56264
200 µg/ml
$322.00

On your images, I see that sometimes this antibody shows more than one band? Is it a phosphorylated or other isoforms or the other genes with ~40% similarity

Asked by: Hima
Thank you for your question. The antibody does undergo a post-transnational modification which is phosphorylation. https://www.uniprot.org/uniprotkb/P30304/entry#ptm_processing
Answered by: BlakeJ
Date published: 2024-04-04

I am using Cdc25A (DCS-120): sc-56264 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Boya_Li from We really enjoyedGreat IHC staining, it is better than the other, play a large role in the experiment.
Date published: 2017-04-10
Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of Cdc25A expression in HeLa and K-562 whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by MC from Great cytoplasmic and membrane immunoperoxidaseGreat cytoplasmic and membrane immunoperoxidase staining observed on glandular cells of formalin fixed, paraffin embedded (FFPE) human upper stomach tissue. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by CJ from Specificity shown for Cdc25A by testing bySpecificity shown for Cdc25A by testing by Western Blot comparing 293T cells that have been transfected with the human Cdc25A protein to those that were not. No band observed for non-transfected cells, correct band observed for cells that were over-expressing the protein. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by JM from Very strong signal by Western Blot usingVery strong signal by Western Blot using HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2013-05-17
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Cdc25A 抗体 (DCS-120) is rated 5.0 out of 5 by 5.
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