Date published: 2026-10-22

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Cdc20 抗体 (H-7): sc-5296

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说明书
  • Cdc20抗体(H-7)是小鼠单克隆IgG2b κ, 在43篇文献中引用,规格为200 µg/ml
  • 免疫human物种的p55 CDC的氨基酸1-175
  • 抗-Cdc20 抗体 (H-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Cdc20
  • 抗Cdc20抗体(H-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Cdc20 Antibody (H-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Cdc20 Antibody (H-7) 打包提供(请参阅下面的订购信息)。
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関連項目

Cdc20抗体(H-7)是一种鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类的Cdc20。Cdc20 (H-7) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdc20在细胞周期调节中发挥着至关重要的作用,特别是在从G2期到有丝分裂的过渡中,它通过激活对细胞周期蛋白和其他调节蛋白的降解至关重要的后端促进复合物/环状体(APC/C)来发挥作用。这种降解对于细胞周期的正常进行至关重要,可确保细胞不会过早进入有丝分裂,从而避免导致基因组不稳定和癌症。Cdc20在活跃分裂的细胞中高度表达,当细胞生长停止时,其表达水平会显著降低,这凸显了其在细胞增殖中的重要性。Cdc20与多种蛋白相互作用,包括细胞周期蛋白B和p34Cdc2激酶,形成对激活M期促进因子(MPF)至关重要的复合物,进一步强调了其在细胞周期控制中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc20 抗体 (H-7) 参考文献:

  1. CDC20通过Bcl-2/Bax通路调节P53突变型肝癌细胞的细胞增殖和放射敏感性。  |  Zhao, S., et al. 2021. Int J Biol Sci. 17: 3608-3621. PMID: 34512169
  2. CDC20在细胞有丝分裂中的内外作用:血液系统恶性肿瘤中的预后因素和治疗靶点。  |  Bruno, S., et al. 2022. J Exp Clin Cancer Res. 41: 159. PMID: 35490245
  3. Cdc20 磷酸化在有丝分裂过程中调节 APC/C 作用的作用。  |  Shevah-Sitry, D., et al. 2022. Proc Natl Acad Sci U S A. 119: e2210367119. PMID: 36001690
  4. PRMT6-CDC20 通过降解 CDKN1B 促进胶质母细胞瘤的进展。  |  Wang, J., et al. 2023. Oncogene. 42: 1088-1100. PMID: 36792756
  5. 抑制 CDC20 可通过促进 GSDME 介导的前列腺癌热蛋白沉积,增强抗肿瘤免疫力。  |  Wu, F., et al. 2023. Exp Hematol Oncol. 12: 67. PMID: 37528490
  6. CDC20通过激活Twist1表达促进前列腺癌的放射抗性。  |  Yang, C., et al. 2023. Apoptosis. 28: 1584-1595. PMID: 37535214
  7. CDC20在肿瘤发生、癌症进展和治疗中的潜在作用:叙述性综述。  |  Xian, F., et al. 2023. Medicine (Baltimore). 102: e35038. PMID: 37682144
  8. 通过靶向适配子增强载有CDC20的ZIF-8纳米粒子包裹在红细胞膜中的骨再生。  |  Du, Y., et al. 2024. Adv Healthc Mater. 13: e2302725. PMID: 38030141
  9. 泛素E3连接酶APC/CCdc20介导OGT的有丝分裂降解。  |  Meng, L., et al. 2024. J Biol Chem. 300: 107448. PMID: 38844135
  10. WWP2缺失通过靶向CDC20/自噬轴加重急性肾损伤。  |  You, R., et al. 2024. J Adv Res.. PMID: 38909885

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc20 抗体 (H-7)

sc-5296
200 µg/ml
$322.00

Cdc20 (H-7): m-IgG Fc BP-HRP 套装

sc-528151
200 µg Ab; 10 µg BP
$361.00

Cdc20 (H-7): m-IgGκ BP-HRP 套装

sc-520459
200 µg Ab, 40 µg BP
$361.00

Cdc20 (H-7): m-IgG2b BP-HRP 套装

sc-550000
200 µg Ab; 10 µg BP
$361.00

Cdc20 抗体 (H-7) AC

sc-5296 AC
500 µg/ml, 25% agarose
$424.00

Cdc20 抗体 (H-7) HRP

sc-5296 HRP
200 µg/ml
$322.00

Cdc20 抗体 (H-7) FITC

sc-5296 FITC
200 µg/ml
$336.00

Cdc20 抗体 (H-7) PE

sc-5296 PE
200 µg/ml
$349.00

Cdc20 抗体 (H-7) Alexa Fluor® 488

sc-5296 AF488
200 µg/ml
$364.00

Cdc20 抗体 (H-7) Alexa Fluor® 546

sc-5296 AF546
200 µg/ml
$364.00

Cdc20 抗体 (H-7) Alexa Fluor® 594

sc-5296 AF594
200 µg/ml
$364.00

Cdc20 抗体 (H-7) Alexa Fluor® 647

sc-5296 AF647
200 µg/ml
$364.00

Cdc20 抗体 (H-7) Alexa Fluor® 680

sc-5296 AF680
200 µg/ml
$364.00

Cdc20 抗体 (H-7) Alexa Fluor® 790

sc-5296 AF790
200 µg/ml
$364.00

What is the total volume provided of p55 CDC (H-7): sc-5296 monoclonal antibody?

Asked by: jenni
Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by Professor Griffin from Great monoclonal antibodyGood results with p55 CDC Antibody (H-7) by Western blot and IF
Date published: 2017-01-11
Rated 5 out of 5 by LL from Excellent results using the antibody in immunofluorescenceExcellent results using the antibody in immunofluorescence (either methanol or PFA fixation).
Date published: 2015-06-08
Rated 5 out of 5 by LL from Satisfied with results by immunoprecipitationSatisfied with results by immunoprecipitation.
Date published: 2015-06-08
Rated 5 out of 5 by LL from Obtained good results in Western BlotObtained good results in Western Blot.
Date published: 2015-06-08
Rated 5 out of 5 by LR from Publishable data in coimmunoprecipitationPublishable data in coimmunoprecipitation and Western blotting with this antibody human HeLA cells. -SCBT Publication Review
Date published: 2015-05-26
Rated 5 out of 5 by JM from Good IF data with mouse testis cell samplesGood IF data with mouse testis cell samples. -SCBT Publication Review
Date published: 2015-05-14
Rated 4 out of 5 by JT from This antibody works well and can be usedThis antibody works well and can be used for IP with human Saos-2, HeLa and MRC5. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by JM from WB positive with human HCT116WB positive with human HCT116, HeLa, and Cos-7 cells. -SCBT Publication Review
Date published: 2015-04-06
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Cdc20 抗体 (H-7) is rated 4.8 out of 5 by 12.
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