Date published: 2026-10-22

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Cdc20 抗体 (AR12): sc-53398

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说明书
  • Cdc20抗体(AR12)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 针对尿素变性His6 Cdc20重组蛋白的抗体,源自human
  • 推荐用于 mouse, rat 和 human 来源的Cdc20 WB, IP, IF 和 IHC(P)检测
  • 看 Cdc20 (E-7): sc-13162,可查看有标记的 Cdc20 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Cdc20 Antibody (AR12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Cdc20 Antibody (AR12) 打包提供(请参阅下面的订购信息)。
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细胞周期蛋白是与激酶结合的调节亚单位,控制着细胞周期进程中的许多重要步骤。Cdc2蛋白激酶(p34Cdc2)在体外表现出蛋白激酶活性,并与细胞周期蛋白B和与p13suc1同源的蛋白质存在于复合物中。Cdc2激酶是M期促进因子(MPF)的活性亚单位和M期特异性组蛋白H1激酶。p34Cdc2/细胞周期蛋白B复合物是G2到M期过渡所必需的。另一种称为Cdc20的细胞周期依赖性蛋白激酶与酿酒酵母蛋白Cdc20和Cdc4具有高度同源性。Cdc20转录本在生长阶段的各种培养细胞系中易于检测,但在细胞生长被化学抑制时消失。Cdc20对α-酪蛋白和髓鞘碱性蛋白表现出激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc20 抗体 (AR12) 参考文献:

  1. 纺锤体检查点对APC-Cdc20的调节。  |  Yu, H. 2002. Curr Opin Cell Biol. 14: 706-14. PMID: 12473343
  2. Cyclin A and Nek2A: APC/C-Cdc20 substrates invisible to the mitotic spindle checkpoint.  |  van Zon, W. and Wolthuis, RM. 2010. Biochem Soc Trans. 38: 72-7. PMID: 20074038
  3. CDC20维持肿瘤起始细胞。  |  Xie, Q., et al. 2015. Oncotarget. 6: 13241-54. PMID: 25938542
  4. BubR1 对 Cdc20 抑制和激活的耦合作用。  |  Hein, JB., et al. 2021. J Cell Biol. 220: PMID: 33819340
  5. p53 直接下调 CDC20 的表达,从而在套细胞淋巴瘤中发挥抗肿瘤活性。  |  Chen, Y., et al. 2023. Exp Hematol Oncol. 12: 28. PMID: 36882855
  6. 人类黑色素瘤中反复出现的CDC20启动子变体的功能分析。  |  Godoy, PM., et al. 2023. Commun Biol. 6: 1216. PMID: 38030698
  7. Cdc20的功能分析揭示了CRY盒在细胞有丝分裂检查点信号传导中的关键作用。  |  Zhang, Y., et al. 2024. Commun Biol. 7: 164. PMID: 38337031
  8. 生物信息学分析和实验验证揭示了CDC20的过度表达会促进膀胱癌的发展和潜在的基本机制。  |  Liu, Y., et al. 2024. Genes Genomics. 46: 437-449. PMID: 38438666
  9. 在DNA损伤的不同阶段,CDC20在RPA1中具有新的调节机制,可诱导放射化疗耐药性。  |  Gao, Y., et al. 2024. Int J Mol Sci. 25: PMID: 39125953
  10. 细胞有丝分裂检查点复合物控制着细胞有丝分裂中Cdc20调节蛋白与泛素连接酶APC/C的结合。  |  Sitry-Shevah, D., et al. 2024. Proc Natl Acad Sci U S A. 121: e2413089121. PMID: 39231204

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc20 抗体 (AR12)

sc-53398
200 µg/ml
$322.00

Cdc20 (AR12): m-IgG Fc BP-HRP 套装

sc-536954
200 µg Ab; 10 µg BP
$361.00

Cdc20 (AR12): m-IgGκ BP-HRP 套装

sc-534131
200 µg Ab; 40 µg BP
$361.00

Cdc20 (AR12): m-IgG1 BP-HRP 套装

sc-544920
200 µg Ab; 20 µg BP
$361.00

Can p55 CDC (AR12): sc-53398 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, p55 CDC (AR12): sc-53398 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by SS from Decent WB signal with some background achievedDecent WB signal with some background achieved using HeLa, Nocodazole treated HeLa and Ramos whole cell lysates. -SCBT QC
Date published: 2015-06-25
Rated 4 out of 5 by SM from Clear immunoperoxidase cytoplasmic stainingClear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by JT from Performs well in WesternPerforms well in Western, good signal with U87MG cells and U373MG. -SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by HF from Very favorable WB achieved using 293T wholeVery favorable WB achieved using 293T whole cell lysate. -SCBT QC
Date published: 2015-01-19
Rated 4 out of 5 by JL from WB publishable data with human U87MG cellsWB publishable data with human U87MG cells and U373MG - SCBT Publication Review
Date published: 2015-01-04
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Cdc20抗体 (AR12) is rated 4.2 out of 5 by 5.
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