Date published: 2025-9-7
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CD9 Antibody (C-4) - Immunohistochemistry - Image 156738
CD9 抗体 (C-4): sc-13118. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色, 显示肾小管细胞膜和细胞质染色.
CD9 抗体 (C-4): sc-13118. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色, 显示肾小管细胞膜和细胞质染色.

CD9 抗体 (C-4): sc-13118

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说明书
  • CD9 抗体 C-4 是小鼠单克隆 IgG1 κ，CD9抗体, 在323篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD9的氨基酸101-210
  • CD9 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 CD9
  • 抗CD9抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取CD9 (C-4): sc-13118的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD9 抗体 (C-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD9 抗体 (C-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 CD9 抗体包括 CD9P-1

CD9抗体（C-4）是一种小鼠单克隆IgG1 kappa轻链抗体，针对人类CD9的氨基酸101-210，可与小鼠、大鼠和人类发生反应，并经过验证可用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）在内的多种应用。CD9，也称为Tetraspanin-29或p24，是一种IV型跨膜糖蛋白，具有四个跨膜结构域，广泛表达于多种细胞表面，包括早期B细胞、嗜酸性粒细胞、嗜碱性粒细胞、活化T细胞和血小板。CD9是细胞间粘附的组成部分，在免疫反应和细胞通讯中尤其重要。此外，CD9在信号传导中发挥着重要作用，通过与低分子量GTP结合蛋白相互作用来影响细胞内信号传导通路。CD9还参与细胞迁移、粘附和融合的调节，这些过程对于免疫功能、组织修复和发育至关重要。CD9与一系列病理状况有关，在非T急性淋巴细胞白血病细胞和一些急性髓细胞白血病和慢性淋巴细胞白血病中表达，这凸显了CD9作为某些血液系统恶性肿瘤标记物的重要性。作为血小板表面的重要组成部分，CD9在血小板聚集和凝块形成中至关重要。抗CD9抗体（C-4）是研究这种关键糖蛋白在健康和疾病中的功能和调节机制的可靠工具。CD9 (C-4)抗体有非结合型和结合型两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和Alexa Fluor®选项，为各种实验应用提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD9 抗体 (C-4) 参考文献:

  1. 急性非淋巴性白血病细胞上的 CD9 抗原：前髓细胞（M3）亚型的优先表达。  |  Ferrero, D., et al. 1991. Leuk Res. 15: 457-61. PMID: 1861531
  2. 有证据表明，信号启动膜蛋白 CD9 与小 GTP 结合蛋白有关。  |  Seehafer, JG. and Shaw, AR. 1991. Biochem Biophys Res Commun. 179: 401-6. PMID: 1909120
  3. 血小板 p24/CD9 的 cDNA 克隆和表达。多跨膜蛋白新家族的证据。  |  Lanza, F., et al. 1991. J Biol Chem. 266: 10638-45. PMID: 2037603
  4. CD9 调节的粘附。抗 CD9 单克隆抗体通过重新识别纤维粘连蛋白诱导前 B 细胞粘附到骨髓成纤维细胞。  |  Masellis-Smith, A. and Shaw, AR. 1994. J Immunol. 152: 2768-77. PMID: 7511626
  5. 跨膜 4 超家族的来龙去脉。  |  Wright, MD. and Tomlinson, MG. 1994. Immunol Today. 15: 588-94. PMID: 7531445
  6. CD9, CD32 和 p67 信号分析：使用脱颗粒血小板表明 CD9 和 p67 直接参与整合素激活。  |  Slupsky, JR., et al. 1997. Br J Haematol. 96: 275-86. PMID: 9029013
  7. TPA 处理 K562 细胞时 CD9 表达上调。  |  Le Naour, F., et al. 1997. Leukemia. 11: 1290-7. PMID: 9264383
  8. 反刍动物白细胞分化抗原的命名和特征。  |  Naessens, J., et al. 1997. Immunol Today. 18: 365-8. PMID: 9267076

订购信息

产品名称产品编号规格价格数量收藏夹

CD9 抗体 (C-4)

sc-13118
200 µg/ml
$316.00

CD9 (C-4): m-IgG Fc BP-HRP 套装

sc-526538
200 µg Ab; 10 µg BP
$354.00

CD9 (C-4): m-IgG1 BP-HRP 套装

sc-531911
200 µg Ab; 20 µg BP
$354.00

CD9 抗体 (C-4) AC

sc-13118 AC
500 µg/ml, 25% agarose
$416.00

CD9 抗体 (C-4) HRP

sc-13118 HRP
200 µg/ml
$316.00

CD9 抗体 (C-4) FITC

sc-13118 FITC
200 µg/ml
$330.00

CD9 抗体 (C-4) PE

sc-13118 PE
200 µg/ml
$343.00

CD9 抗体 (C-4) Alexa Fluor® 488

sc-13118 AF488
200 µg/ml
$357.00

CD9 抗体 (C-4) Alexa Fluor® 546

sc-13118 AF546
200 µg/ml
$357.00

CD9 抗体 (C-4) Alexa Fluor® 594

sc-13118 AF594
200 µg/ml
$357.00

CD9 抗体 (C-4) Alexa Fluor® 647

sc-13118 AF647
200 µg/ml
$357.00

CD9 抗体 (C-4) Alexa Fluor® 680

sc-13118 AF680
200 µg/ml
$357.00

CD9 抗体 (C-4) Alexa Fluor® 790

sc-13118 AF790
200 µg/ml
$357.00

CD9 抗体 (C-4) Alexa Fluor® 405

sc-13118 AF405
200 µg/ml
$357.00

Hello. Could you please confirm if the CD9 (C-4) antibody is compatible with the m-IgGκ BP-HRP secondary antibody?

Asked by: nme75
Thank you for your question. CD9 Antibody (C-4) is a mouse monoclonal IgG1 κ CD9 antibody so m-IgGκ BP-HRP: sc-516102 can indeed be used to detect it.
Answered by: Tech Support Europe
Date published: 2025-08-16

Good morning Excuse me, I'm interested in buying the p27 antibody, but I would like to know if there is a way to obtain a sample to determine the affinity for the technique and then buy it

Asked by: Alan Hipólito Juárez Sola
Thank you for your question. Please contact technical service to receive a sample . Please email scbt@scbt.com or call (800)-457-3801
Answered by: BlakeJ
Date published: 2023-02-23

Does this AB run under reducing or non-reducing condition?

Asked by: NPMerck
Thank you for your question. This antibody detects CD9 in both reducing and non-reducing conditions.
Answered by: Tech Support Europe
Date published: 2020-11-19

Dear santa cruz, I am asking for a quotation to the American University in Cairo for exosomal CD9 antibody for MCF-7 and MCF-ADR cells. Best regards, Mohammed Hesham

Asked by: Anonymous
Thank you for your question. For accurate availability, shipping, and pricing information regarding our antibodies, or to receive a quote, please contact your local distributor.
Answered by: Technical Service 4
Date published: 2020-11-12

can we use this antibody in detetection exosomes using flowcytometry?

Asked by: ganesha
Thank you for your question. Yes, this mouse monoclonal CD9 antibody is recommended for Flow Cytometry.
Answered by: Tech Service
Date published: 2020-01-03

I would like to ask which clone this antibody is?

Asked by: SarahD123
Thank you for your question. This is a mouse monoclonal IgG1 against CD9 and the clone name is (C-4). This clone is exclusively developed and offered by Santa Cruz Biotechnology.
Answered by: Technical Support
Date published: 2022-03-21

Can it be used for detecting exosome from mouse serum?

Asked by: Karen Z
Thank you for your question. This mouse monoclonal antibody, CD9 (C-4): sc-13118, is recommended for detection of CD9 of mouse, rat and human origin. We do not have any information about expression of CD9 in exosomes.
Answered by: Technical Support
Date published: 2023-01-18

Can I use this antibody as a capture antibody for ELISA. Also, can it bind to protein A coated plate.

Asked by: Pooja Dua
Thank you for your question. This antibody has not been tested for sandwich ELISA, but we would expect it to be fine for that use. It should be able to be coated onto a protein A coated plate without a problem. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-08-31
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Rated 3 out of 5 by from IHC DiscretaLo staining su tessuto paraffinato umano è abbastanza soddisfacente. Ho usato un retrival antigenico con EDTA pH 8.0 per 25' e una diluizione di 1:400 su linfonodo. Rimane dello staining di background aspecifico
Date published: 2023-01-23
Rated 5 out of 5 by from WBできれいなバンドが検出できました。ウエスタンブロットでバックグラウンドが低くきれいなバンドが検出できました。5%スキムミルクと市販のブロッキング剤で比較しても大きく差はありませんでした。
Date published: 2021-09-10
Rated 1 out of 5 by from Not workingI can not see any band on my WB using 1:500 dilution
Date published: 2020-06-29
Rated 2 out of 5 by from Works in WB analysis (weak signal)Dilution 1:200 in 5% Milk/TBST, incubation OVN at 4°C, specific band, very weak signal
Date published: 2019-11-10
Rated 2 out of 5 by from Weak bands in WBVery weak bands in HCT116-derived EVs(Dilution 1:100 in 5% Milk/TBST)
Date published: 2019-09-04
Rated 1 out of 5 by from Pas de résultatsJ'ai testé cet anticorps sur des extrait cellulaire et n'ai obtenu aucun résultat. Je tente de faire des ajustements dans le protocole mais pour l'instant pas très concluant
Date published: 2019-03-05
Rated 4 out of 5 by from Works for Western blotting of CD9 in MCF7I tried a sample of this antibody for a western blot on MCF7 total cell lysates and exosomal purifications, at a:1000 dilution in TBST-5%milk. Both gave a clear and unique signal with ECL (but best with Femto reagent - Pierce, as seen on picture)
Date published: 2019-01-31
Rated 5 out of 5 by from Great product, highly recommended.It was the first time that I tried this antibody, and it works great for my ICC staining. Please see the attached confocal image. I used 1:50 dilution this time, the signal is not very strong but staining pattern is exactly I expected, which shows that the majority of CD9 is located on the cell membrane. I will try 1:25 dilution next time to see whether it can make it even better. I feel the overall quality of SCBT antibodies has been improved quite a lot, and the price is very competitive compared to many other vendors because the supplied volume is efficient for multiple experiments. Only one concern is that SCBT discontinued almost all polyclonal antibodies, which means I only can get those polyclonal antibodies from other suppliers if I need to do co-staining with 2-3 antibodies.
Date published: 2018-11-08
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CD9 抗体 (C-4) is rated 4.1 out of 5 by 39.
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