Date published: 2025-9-6

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CD26 抗体 (5E8): sc-52642

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说明书
  • CD26 抗体 5E8 是小鼠单克隆 IgG1 κ,CD26抗体, 在18篇文献中引用,规格为200 µg/ml
  • 对rat来源的肝质膜提取物
  • CD26 抗体 (5E8) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse 和rat 来源的 CD26
  • 抗CD26抗体(5E8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD26 (5E8): sc-52642免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CD26 抗体 (5E8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD26 抗体 (5E8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD26 抗体 (5E8) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和免疫组织化学方法检测小鼠和大鼠来源的 CD26 蛋白。抗 CD26 抗体 (5E8) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。CD26 又称二肽基肽酶 4、腺苷脱氨酶复合蛋白 2 和 DPPIV,是一种膜糖蛋白和丝氨酸外肽酶,通过裂解多肽 N 端的 X-脯氨酸二肽在免疫调节中发挥重要作用。CD26 主要位于 T 细胞表面,作为一种共刺激分子,它能增强 T 细胞的活化并调节趋化因子的活性。这种定位对免疫反应至关重要,因为 CD26 与包括 CXCR4 和 gp120 在内的多种蛋白质相互作用,而这些蛋白质与艾滋病病毒感染的病理生理学有关。CD26 与树突状细胞上的腺苷脱氨酶相互作用,促进了免疫突触中的成本刺激信号,促进了促炎细胞因子的产生,如γ干扰素、肿瘤坏死因子α和白细胞介素-6。CD26 参与了各种人类癌症的发病机制,影响着实体瘤和血液恶性肿瘤的行为。CD26 与洞穴素-1 之间的相互作用有助于上调单核细胞上的 CD86,而 CD86 是有效激活抗原特异性 T 细胞的关键。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD26 抗体 (5E8) 参考文献:

  1. 干扰素和维甲酸对肿瘤 B 细胞中 CD26/DPPIV 基因表达的调控  |  Bauvois, B., et al. 2000. Oncogene. 19: 265-72. PMID: 10645005
  2. 6- 磷酸甘露糖/类胰岛素生长因子 II 受体对 CD26 的内化有助于 T 细胞活化。  |  Ikushima, H., et al. 2000. Proc Natl Acad Sci U S A. 97: 8439-44. PMID: 10900005
  3. 人类淋巴细胞中 CXCR4 和 CD26 的共同调节。  |  Herrera, C., et al. 2001. J Biol Chem. 276: 19532-9. PMID: 11278278
  4. T 细胞激活抗原 CD26 的克隆和功能表达。  |  Tanaka, T., et al. 1992. J Immunol. 149: 481-6. PMID: 1352530
  5. 类风湿滑膜通过 CD26 和洞穴素-1 激活 T 细胞  |  Ohnuma, K., et al. 2006. Mod Rheumatol. 16: 3-13. PMID: 16622717
  6. 1F7 (CD26):胸腺成熟的标志物,参与人类胸腺细胞活化的 CD3 和 CD2 途径的不同调节。  |  Dang, NH., et al. 1991. J Immunol. 147: 2825-32. PMID: 1717577
  7. 鉴定大鼠胸腺中的 Ia 糖蛋白并从大鼠脾脏中纯化。  |  McMaster, WR. and Williams, AF. 1979. Eur J Immunol. 9: 426-33. PMID: 315315
  8. 二肽基肽酶 4 (CD26) 与膝骨关节炎软骨细胞衰老和放射学进展的关系  |  Chen, YH., et al. 2023. Arthritis Rheumatol. 75: 1120-1131. PMID: 36704903
  9. CD26:一种参与 T 细胞活化的表面蛋白酶。  |  Fleischer, B. 1994. Immunol Today. 15: 180-4. PMID: 7911022
  10. 人类 CD26(二肽基肽酶 IV)基因的基因组组织, 准确定域和组织表达。  |  Abbott, CA., et al. 1994. Immunogenetics. 40: 331-8. PMID: 7927537
  11. CD26 对 CD4 记忆 T 细胞功能和活化的作用  |  Dong, RP. and Morimoto, C. 1996. Hum Cell. 9: 153-62. PMID: 9183643
  12. 淋巴细胞生长调节中的 CD26/二肽基肽酶 IV。  |  Ansorge, S., et al. 1997. Adv Exp Med Biol. 421: 127-40. PMID: 9330689

订购信息

产品名称产品编号规格价格数量收藏夹

CD26 抗体 (5E8)

sc-52642
200 µg/ml
$316.00

CD26 (5E8): m-IgG Fc BP-HRP 套装

sc-528544
200 µg Ab; 10 µg BP
$354.00

CD26 (5E8): m-IgGκ BP-HRP 套装

sc-520942
200 µg Ab, 40 µg BP
$354.00

CD26 (5E8): m-IgG1 BP-HRP 套装

sc-542984
200 µg Ab; 20 µg BP
$354.00

CD26 抗体 (5E8) AC

sc-52642 AC
500 µg/ml, 25% agarose
$416.00

CD26 抗体 (5E8) HRP

sc-52642 HRP
200 µg/ml
$316.00

CD26 抗体 (5E8) FITC

sc-52642 FITC
200 µg/ml
$330.00

CD26 抗体 (5E8) PE

sc-52642 PE
200 µg/ml
$343.00

CD26 抗体 (5E8) Alexa Fluor® 488

sc-52642 AF488
200 µg/ml
$357.00

CD26 抗体 (5E8) Alexa Fluor® 546

sc-52642 AF546
200 µg/ml
$357.00

CD26 抗体 (5E8) Alexa Fluor® 594

sc-52642 AF594
200 µg/ml
$357.00

CD26 抗体 (5E8) Alexa Fluor® 647

sc-52642 AF647
200 µg/ml
$357.00

CD26 抗体 (5E8) Alexa Fluor® 680

sc-52642 AF680
200 µg/ml
$357.00

CD26 抗体 (5E8) Alexa Fluor® 790

sc-52642 AF790
200 µg/ml
$357.00

I have use CD26 Antibody (5E8): sc-52642 on mouse kidney sections for IHC staining. Unfortunately, I didnot get any staining in proximal tubules of mice kidney. I checked all steps of protocol but still no staining

Asked by: b1978
Thank you for your question. CD26 Antibody (5E8): sc-52642 is a mouse monoclonal antibody that is suitable for detection of CD26 on mouse kidney formalin-fixed paraffin-embedded tissue sections. We have data on our website in rat kidney tissue showing apical membrane staining of cells in tubules. A link to our recommended protocol is provided below. Please contact our Technical Service Department if you would like help with troubleshooting the antibody. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-05-24

Can CD26 (5E8): sc-52642 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD26 (5E8): sc-52642 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

这支抗体除了识别大鼠组织,在WB试验中,可以用于Human来源的细胞蛋白么? Does this antibody recognize protein from human cells?

Asked by: muyuzihegui
感谢您的提问。您可以联系我们的技术支持部门,我们会帮您进行序列比对分析,以确定该抗体能否用于您的样本。亚洲地区技术支持电话(+86) 021-60936351,邮箱asia@scbio.cn。 Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Technical Services (Aisa): (+86) 021-60936351 or asia@scbio.cn.
Answered by: Technical Support 12
Date published: 2017-01-06
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Rated 5 out of 5 by from Ottimo funzionamentoHo acquistato questo prodotto e sono molto soddisfatta
Date published: 2022-04-01
Rated 2 out of 5 by from Faint band...Tested in hepatic Huh 7.5 cells. Numerous faint bands, band at expected size extremely faint..
Date published: 2017-08-31
Rated 5 out of 5 by from 条带清晰I have used this product one month ago.It is very clear in western bloting,very good!
Date published: 2017-01-17
Rated 5 out of 5 by from Positive result in WB in rat kidney tissuePositive result in WB in rat kidney tissue extract. -SCBT QC
Date published: 2015-05-30
Rated 5 out of 5 by from Band of interest observed in WB in rat PBLBand of interest observed in WB in rat PBL whole cell lysate. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Publishable data with rat liver samplePublishable data with rat liver sample - SCBT Publication Review
Date published: 2014-03-19
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CD26 抗体 (5E8) is rated 4.5 out of 5 by 6.
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