CD16 Antibody (YFC 120.5): sc-58962

CD16 Antibody (YFC 120.5) is a rat monoclonal IgG2b antibody designed to detect CD16, also known as Fc gamma receptor III (FcγRIII), across mouse, rat, and human species using applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD16 exists in two primary isoforms: CD16A (FcγRIIIA), a transmembrane protein localized on the surface of natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein found on neutrophils. The specific cellular localization of CD16 is essential for its function; CD16A′s presence on NK cells and macrophages facilitates antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis, while CD16B′s anchoring on neutrophils plays a pivotal role in pathogen clearance and inflammatory responses. These distinct locations enable CD16 to interact with different signaling molecules and pathways, thereby orchestrating effective immune responses. CD16 monoclonal antibody (YFC 120.5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for a wide range of experimental setups. Anti-CD16 antibody (YFC 120.5) is indispensable for research into immune cell function, autoimmune diseases, and conditions such as paroxysmal nocturnal hemoglobinuria (PNH), where CD16 expression is significantly altered. With alternate names including FcγRIII, Fc gamma receptor III, CD16A, and CD16B, CD16 monoclonal antibody (YFC 120.5) offers comprehensive detection capabilities, enabling detailed studies of CD16′s role and regulation within the immune system.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD16 Antibody (YFC 120.5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CD16 Antibody (YFC 120.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD16 Antibody (YFC 120.5) Specifications

Alternate Product Name: CD16

Product Clone Name: YFC 120.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: CD16

Epitope Species: human

Product Reactivity/Detection: CD16-A and CD16-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CD16 Antibody (YFC 120.5): AF594: Alexa Fluor^® 594, FITC: Fluoroscein, PE: Phycoerythrin

The preferred CD16 antibody is CD16 Antibody (DJ130c): sc-20052.