CD16 Antibody (B-E16): sc-65294
- CD16 Antibody (B-E16) is a mouse monoclonal IgG2a provided at 100 µg/ml
- raised against natrual killer cells
- recommended for detection of CD16-A and CD16-B of human origin by WB, IP and FCM
- available conjugated to phycoerythrin for IF, IHC(P) and FCM
- See CD16 (DJ130c): sc-20052 for CD16 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD16 Antibody (B-E16). This work is in progress.
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CD16 Antibody (B-E16) is a mouse monoclonal IgG2a antibody designed to detect human CD16, also known as Fc gamma receptor III (FcγRIII), through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). CD16 is a critical immune receptor that exists in two primary isoforms: CD16A (FcγRIIIA), a transmembrane protein found on natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein present on neutrophils. The structural differences between these isoforms are essential for their distinct functions; CD16A′s transmembrane domain allows engagement in robust signaling pathways by associating with the γ subunit of FcεRI or the ζ subunit of the TCR-CD3 complex, which are crucial for initiating immune responses such as antibody-dependent cellular cytotoxicity (ADCC). In contrast, CD16B′s GPI anchor facilitates localization on the cell surface, enabling effective interaction with IgG antibodies to mediate phagocytosis and pathogen clearance. These structural features determine the receptor′s ability to participate in various immune processes, highlighting CD16′s importance in maintaining immune homeostasis and defense mechanisms. CD16 monoclonal antibody (B-E16) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for diverse experimental needs. Anti-CD16 antibody (B-E16) is indispensable for research into immune cell function, autoimmune diseases, and conditions such as paroxysmal nocturnal hemoglobinuria (PNH), where CD16 expression is significantly altered. With alternate names including FcγRIII, Fc gamma receptor III, CD16A, and CD16B, CD16 (B-E16) antibody offers comprehensive detection capabilities, enabling detailed studies of CD16′s structural roles and functional mechanisms within the immune system.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD16 Antibody (B-E16) | sc-65294 | 100 µg/ml | $322.00 | |||
CD16 Antibody (B-E16) PE | sc-65294 PE | 100 tests in 2 ml | $349.00 |