CD16 Antibody (2Q1240): sc-70548

CD16 Antibody (2Q1240) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect human CD16, also known as Fc gamma receptor III (FcγRIII), across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD16 plays a crucial role in the immune system by binding the Fc region of IgG antibodies, thereby mediating essential functions such as antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. This receptor exists in two main isoforms: CD16A (FcγRIIIA), a transmembrane protein expressed on natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein found on neutrophils. Post-translational modifications are vital for CD16′s functionality and localization; for instance, the glycosylation of CD16A enhances its stability and affinity for IgG, while the GPI anchor of CD16B facilitates its attachment to the neutrophil membrane, enabling effective signal transduction and immune responses. These modifications ensure that each isoform can interact appropriately with intracellular signaling molecules, such as the γ subunit of FcεRI or the ζ subunit of the TCR-CD3 complex for CD16A, which are essential for initiating robust immune responses. CD16 (2Q1240) monoclonal antibody is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for diverse experimental setups. CD16 (2Q1240) antibody is indispensable for research into immune cell function, autoimmune diseases, and conditions such as paroxysmal nocturnal hemoglobinuria (PNH), where CD16 expression is significantly altered. With alternate names including FcγRIII, Fc gamma receptor III, CD16A, and CD16B, CD16 (2Q1240) monoclonal antibody offers comprehensive detection capabilities, enabling detailed studies of CD16′s post-translational modifications and their impact on immune regulation and response.

The preferred CD16 antibody is CD16 Antibody (DJ130c): sc-20052.